Quality evaluation of different varieties of rhubarb based on multicomponents and bioactivity: Application to quality control in the production of rhubarb decoction pieces.

In this work, we developed a novel HPLC method for the simultaneous determination of 16 bioactive components in rhubarb and used it to determine the contents. Through the determination of bioactive components and in vitro activity, we compared the differences between 18 batches of different varieties of rhubarb. The contents of 16 bioactive components in Rheum officinale Baill., Rheum tanguticum Maxim. ex Balf., and Rheum palmatum Linn. were 57.53-76.09, 98.88-130.61 and 36.18-48.66 mg/g, respectively. The average inhibition rates of alkaline phosphatase in R. tanguticum were the highest, reaching 79.53%. The average inhibition rates of thrombin and Na+ /K+ -ATPase in R. palmatum were the highest, reaching 80.93 and 62.42%, respectively. These results showed that there were significant differences in effective components and biological activities among different varieties of rhubarb. Through chemometric analysis, we preliminarily compared the differences in chemical composition of different varieties of rhubarb, and established a Fisher discriminant function which could accurately distinguish different varieties of rhubarb. Through correlation analysis and molecular docking, three potential components as quality markers of rhubarb were identified. The obtained results provide a comprehensive strategy for the quality control of multisource Chinese medicine and related products.

Effect of evidence-based nursing on the prognosis and curative effect of patients with hypertensive cerebral hemorrhage / 中国基层医药

Objective To investigate the effect of evidence-based nursing on the clinical curative effect and prognosis of patients with hypertensive cerebral hemorrhage.Methods 120 patients with hypertensive cerebral hemorrhage were selected,and they were randomly divided into observation group(60 cases) and control group(60 cases) according to the digital table.The control group was treated with routine nursing,the observation group was treated with routine nursing and evidence-based nursing.Before and after nursing,the SDS,self rating anxiety scale (SAS),neurological deficit score NIHSS,Barthel score,the incidence of sequelae,hospitalization time,nursing quality score and patients’ satisfaction with nursing were compared between the two groups.Results There were no statistically significant differences in SDS,SAS,NIHSS and Barthel scores between the two groups before nursing intervention(all P ＞ 0.05).After nursing intervention,the SDS,SAS,NIHSS and Barthel scores of the observation group were (38.74 ± 6.21) points,(35.83 ± 8.17) points,(11.24 ± 3.08) points,(92.58 ± 6.46) points,respectively,which in the control group were (44.58 ± 7.10) points,(43.66 ± 8.06) points,(15.34 ± 3.29) points,(84.27 ± 5.82) points,there were significant differences between the two groups (t =4.796,5.285,7.047,7.403,all P ＜0.05).,The incidence rate of venous thrombosis,muscle atrophy and joint ankylosis sequelae of the observation group was 8.33％,which was lower than 40.00％ of the control group,there was significant difference between the two groups(x2 =16.415,P ＜ 0.05).The hospitalization time of the observation group was (9.55 ± 2.43)d,which was shorter than (15.97 ± 4.68) d of the control group (t =9.430,P ＜ 0.05).The health education nursing quality score,ward management score,basic nursing score,nursing care of critical patients score,nursing document writing score of the observation group were (97.66 ± 2.45) points,(98.23 ± 3.46) points,(97.54 ± 3.18) points,(96.88 ± 3.49) points,(98.76 ± 1.31)points,respectively,which were higher than those of the control group [(88.79 ± 2.37) points,(90.72 ±3.52) points,(91.05 ±3.16) points,(91.67 ± 5.34) points,(93.04 ± 1.12) points],there were significant differences between the two groups(t =20.156,11.786,11.214,6.326,25.707,all P ＜ 0.05).The patients’ nursing satisfaction of the observation group (96.67％) was higher than that of the control group (85.00％),there was significant difference between the two groups (x2 =4.904,P ＜ 0.05).Conclusion Evidence -based nursing can effectively relieve patients’ anxiety and depression of patients with hypertensive cerebral hemorrhage,improve the quality of life of patients,reduce the incidence of complications,shorten the hospitalization time,improve patients'satisfaction.