Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Xenotransplantation ; 30(1): e12786, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36367201

RESUMO

Cardiovascular disease is common and has a high mortality. Due to the limited number of organs available for orthotopic heart transplantation, alternative therapies have received intense interest. In this commentary we contrast xenotransplantation and blastocyst complementation to produce pigs that will serve as donors for organ transplantation. These strategies hold tremendous promise and have the potential to provide an unlimited number of organs for chronic, terminal diseases.


Assuntos
Transplante de Coração , Transplante de Órgãos , Transplantes , Animais , Suínos , Humanos , Transplante Heterólogo , Doadores de Tecidos
3.
Front Genet ; 13: 948496, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35957684

RESUMO

Focusing on complex extremity trauma and volumetric muscle loss (VML) injuries, this review highlights: 1) the current pathophysiologic limitations of the injury sequela; 2) the gene editing strategy of the pig as a model that provides a novel treatment approach; 3) the notion that human skeletal muscle derived from gene edited, humanized pigs provides a groundbreaking treatment option; and 4) the impact of this technologic platform and how it will advance to far more multifaceted applications. This review seeks to shed insights on a novel treatment option using gene edited pigs as a platform which is necessary to overcome the clinical challenges and limitations in the field.

4.
J Mol Cell Cardiol ; 172: 109-119, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36030840

RESUMO

End stage heart failure is a terminal disease, and the only curative therapy is orthotopic heart transplantation. Due to limited organ availability, alternative strategies have received intense interest for treatment of patients with advanced heart failure. Recent studies using gene-edited porcine organs suggest that cardiac xenotransplantation may provide a future source of organs. In this review, we highlight the historical milestones for cardiac xenotransplantation and the gene editing strategies designed to overcome immunological barriers, which have culminated in a recent cardiac pig-to-human xenotransplant. We also discuss recent results of studies on the engineering of human-porcine chimeric organs that may provide an alternative and complementary strategy to overcome some of the major immunological barriers to producing a new source of transplantable organs.


Assuntos
Insuficiência Cardíaca , Transplante de Coração , Transplantes , Humanos , Suínos , Animais , Transplante Heterólogo/efeitos adversos , Transplante Heterólogo/métodos , Transplante de Coração/métodos , Insuficiência Cardíaca/genética , Insuficiência Cardíaca/terapia , Edição de Genes
5.
Comp Med ; 67(5): 436-441, 2017 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-28935006

RESUMO

Cynomolgus monkeys are often used in preclinical transplantation research. Performing liver transplantation in cynomolgus monkeys is challenging because they poorly tolerate portal vein clamping during the anhepatic phase. Finding an alternative to portal vein clamping is necessary before preclinical liver transplant models can be performed with reliable outcomes. We used 3 different techniques to perform 5 liver transplants in male cynomolgus macaques (weight, 7.4-10.8 kg; mismatched for MHC I and II; matched for ABO). In procedure A, we clamped the portal vein briefly, as in human transplants, as well as the superior mesentery artery to minimize congestion at the expense of temporary ischemia (n = 2). In procedure B, we performed a temporary portocaval shunt with extracorporeal venovenous bypass (n = 1). For procedure C, we developed an H-shunt system (modified portocaval shunt) with extracorporeal bypass (n = 2). Postoperative immunosuppression comprised cyclosporine A, mycophenolate mofetil, and steroids. Recipients in procedure A developed hemodynamic instability and were euthanized within 2 d. The recipient that underwent procedure B was euthanized within 11 d due to inferior vena caval thrombosis. The H-shunt in procedure C led to minimal PV congestion during the anhepatic phase, and both recipients reached the 21-d survival endpoint with good graft function. Our novel H-shunt bypass system resulted in successful liver transplantation in cynomolgus macaques, with long-term posttransplant survival possible. This technical innovation makes possible the use of cynomolgus monkeys for preclinical liver transplant tolerance models.


Assuntos
Ciclosporina/uso terapêutico , Imunossupressores/uso terapêutico , Transplante de Fígado/veterinária , Macaca fascicularis/cirurgia , Derivação Portocava Cirúrgica/veterinária , Anastomose Cirúrgica/métodos , Anastomose Cirúrgica/veterinária , Animais , Feminino , Humanos , Testes de Função Hepática/veterinária , Transplante de Fígado/efeitos adversos , Transplante de Fígado/métodos , Masculino , Artérias Mesentéricas/cirurgia , Modelos Animais , Veia Porta
6.
Comp Med ; 66(4): 308-23, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27538862

RESUMO

Cynomolgus macaques (CYNO; Macaca fascicularis) are a well-established NHP model used for studies in immunology. To provide reference values on the baseline cell distributions in the hematopoietic and lymphoid organs (HLO) of these animals, we used flow cytometry to analyze the peripheral blood, bone marrow, mesenteric lymph nodes, spleen, and thymus of a cohort of male, adult, research-naïve, Mauritian CYNO. Our findings demonstrate that several cell distribution patterns differ between CYNO and humans. First, the CD4(+):CD8(+) T-cell ratio is lower in CYNO compared with humans. Second, the peripheral blood of CYNO contains a population of CD4(+)CD8(+) T cells. Third, the CD31 level was elevated in all organs studied, suggesting that CD31 may not be an accurate marker of recent thymic emigrants within the CD4(+) T cells of CYNO. Finally the B-cell population is lower in CYNO compared with humans. In summary, although the majority of immune cell populations are similar between cynomolgus macaques and humans, several important differences should be considered when using CYNO in immunologic studies. Our current findings provide valuable information to not only researchers but also veterinarians working with CYNO at research centers, in zoos, or in the wild.


Assuntos
Leucócitos/classificação , Macaca fascicularis/anatomia & histologia , Macaca fascicularis/imunologia , Animais , Antígenos CD34/metabolismo , Linfócitos B/citologia , Relação CD4-CD8 , Antígeno CD56/metabolismo , Fatores de Transcrição Forkhead/metabolismo , Sistema Hematopoético/citologia , Sistema Hematopoético/imunologia , Humanos , Subunidade alfa de Receptor de Interleucina-2/metabolismo , Antígenos Comuns de Leucócito/metabolismo , Leucócitos/citologia , Leucócitos/imunologia , Tecido Linfoide/citologia , Tecido Linfoide/imunologia , Macaca fascicularis/sangue , Masculino , Monócitos/citologia , Especificidade de Órgãos , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Especificidade da Espécie , Subpopulações de Linfócitos T/citologia , Subpopulações de Linfócitos T/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...