RESUMO
The New Jersey Essential Health Services Commission was established in 1992. The author reviews the role of the New Jersey Essential Health Services Commission in health care reform, including a subsidized health benefits program and payments to hospitals for charity care.
Assuntos
Reforma dos Serviços de Saúde/legislação & jurisprudência , Conselhos de Planejamento em Saúde/legislação & jurisprudência , Planos Governamentais de Saúde/legislação & jurisprudência , Controle de Custos/legislação & jurisprudência , Reforma dos Serviços de Saúde/economia , Acessibilidade aos Serviços de Saúde/economia , Acessibilidade aos Serviços de Saúde/legislação & jurisprudência , Humanos , Indigência Médica/economia , Indigência Médica/legislação & jurisprudência , New Jersey , Planos Governamentais de Saúde/economia , Estados UnidosRESUMO
Human meningiomas show characteristic chromosome aberrations very similar to those reported in human cell cultures experimentally transformed by Simian virus 40 (SV 40). Three out of eight meningiomas tested showed SV 40 specific tumor antigen but no virus capsid antigen. Experiments to rescue virus were successfully performed. Electron microscopic studies of these cell cultures revealed cytoplasmic inclusions containing virus particles which corresponded in size and morphology to Papova viruses.
Assuntos
Papillomaviridae/imunologia , Polyomaviridae , Antígenos Virais/análise , Células Cultivadas , Humanos , Corpos de Inclusão , Microscopia Eletrônica , Vírus 40 dos Símios/imunologiaRESUMO
The recently detected reciprocal translocations in chronic myeloic leucemia (CML) and Burkitt's lymphoma (BL) made it necessary to clarify if meningiomas really show the described monosomy 22 or also a translocation. In 10 out of 12 meningiomas a total or partial translocation of the missing chromosome 22 to another chromosome could be ruled out by fluorescence banding analysis. Two meningiomas showed marker chromosomes of such a complex composition that it was impossible to decide if a 22 translocation was present or not. From these results it was concluded that meningioma cells, in contrast to CML and BL, show almost regularly a loss of a definitive part of their genome.
Assuntos
Aneuploidia , Aberrações Cromossômicas , Meningioma/genética , Translocação Genética , Linfoma de Burkitt/genética , Transformação Celular Neoplásica , Deleção Cromossômica , Feminino , Humanos , Leucemia Linfoide/genética , MasculinoRESUMO
Two out of seven meningiomas tested in early cell cultures by indirect immunofluorescence staining showed simian virus 40 (SV40)-related tumor (T) antigen. In one tumor 90% of the cells were positive. An additional SV40-related antigen (U) was found in 10% of cells of a third tumor. These findings indicate that the meningioma cells showing a positive reaction are transformed by a papova virus that has at least partly the same antigenic properties as SV40 virus. SV40-related viral capsid (V) antigen was absent in all the meningiomas tested. No virus infectious for African green monkey kidney (AGMK) cells could be isolated. The tumors positive for T and U antigens showed the chromosome aberration typical for human meningiomas, i.e., the loss of one chromosome, G-22. The T-antigen-positive tumors showed further hypodiploidization. Experiments to rescue virus from the T-antigen-positive tumors showed further hypodiploidization. Experiments to rescue virus from the T-antigen-positive meningioma cells were performed: fusion of cells pretreated with 8-azaguanine with cells premissive for SV40 led to a low percentage (0.01-0.05%) of V-antigen-positive nuclei in heterokaryon cultures. On the basis of these results, the possibility of a correlation between the meningioma, a relatively common intracranial tumor in man, and an SV40-related papova virus must be considered. It remains to be shown whether this virus is a causative agent for human meningiomas.
