1.
Biotechnol Bioeng
; 91(4): 460-7, 2005 Aug 20.
Artigo
em Inglês
| MEDLINE
| ID: mdl-15959893
RESUMO
We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems.