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Objective To study the mechanisms of depression by exploring expressional differences of AQPs in the tissues of chronic stress depression rats.Methods Depression model was replicated by unpredicted chronic stress.20 rats were randomly separated into normal control group and model control group,AQP4 and AQP3,AQP8 expressions on hippocampus and gastrointestinal mucosa of rat model with depression were detected by immunochemical staining method.Results Means of optical density of AQP4 of normal group and model group hippocampus were 0.28 ± 0.02,0.22 ± 0.06 respectively,and the difference between two groups was significant statistically(t value was 2.756,P＜0.05).The expression of AQP3 on gastric mucosa and colonic mucosa between two groups had no significant statistically(t value were 1.814,1.812,P＞0.05).Two groups'means of optical density of AQP3 on small intestinal mucosa were 0.15 ± 0.02,0.17 ± 0.02,and the difference was significant statistically (t value was 2.769,P＜0.05).Two groups'means of AQP8 optical density in gastric mucosa were 0.15± 0.01,0.19 ± 0.04 ;0.16 ± 0.01 and 0.21 ± 0.04 in small intestinal mucosa;0.16 ± 0.01 and 0.22 ± 0.04 in colonic mucosa,and the differences were significant statistically(t values were 3.139,5.113,4.534,P＜0.05,P＜0.01,P＜0.01).Conclusion The expression of AQP4 on depression model rat hippocampus are lower than those of the normal group ; and the expression of AQP3 on gastric mucosa and colonic mucosa are no change obviously,but it(')s up-regulation on small intestinal mucosa.The expressions of AQP8 on gastric mucosa and small intestinal mucosa and colonic mucosa are up-regulating.
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Objective To study the mechanism of depression by explore the expressional differences of synapsin and dopamine transporter(DAT) mRNA of hippocampus in depression model rats. Methods Depression model were replicated by chronic stress. 20 rats were randomly divided into normal control group(n=10) and de-pression model group(n=10). Synapsin and DAT mRNA expressions in hippocampns were detected with RT-PCR technique. Result The values of synapsin and DAT mRNA in hippocampus of normal control group were 0.84±0.08 and 1.24±0.08, respectively, and 1.24±0.12 and 1.85±0.09 of model group, their t values were 8.87 and 16.50 (P<0.01). Conclusion The expressions of synapsin and DAT mRNA in hippecampus of depression model rats were increased significantly.
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92 type 2 diabetics with DPN were treated by cobamamide (n=46) vs vitamin B12 (n=46) for 4 weeks.Cobamamide improved the spontaneous pain, numbness of limbs, nerve reflection and nerve conduction velocities in higher rates as compared with vitamin B12 treatment, and did not cause obvious adverse reactions.
RESUMO
92 type 2 diabetics with DPN were treated by cobamamide (n=46) vs vitamin B12 (n=46) for 4 weeks.Cobamamide improved the spontaneous pain, numbness of limbs, nerve reflection and nerve conduction velocities in higher rates as compared with vitamin B12 treatment, and did not cause obvious adverse reactions.
