Scrotal and oral temperatures are not related to semen quality of serum gonadotropin levels in spinal cord-injured men.

Scrotal temperature, oral temperature, and the difference between oral and scrotal temperature were measured in spinal cord-injured subjects (SCI) and non-injured subjects as controls. We statistically correlated these measures to semen quality and serum gonadotropin levels in both groups. No difference was found between SCI and control subjects on any temperature measurement. Mean sperm motility, mean sperm morphology, and mean serum gonadotropin levels were significantly lower in SCI compared to control subjects, but these measures were not correlated to scrotal temperature, oral temperature, or the difference between oral and scrotal temperature in SCI or control subjects. These data indicate that: 1) there is not a generalized scrotal thermoregulatory dysfunction in SCI men; 2) scrotal temperature does not appear to contribute to poor semen quality in SCI men; and 3) elevated gonadotropin levels are not related to elevated scrotal temperatures in SCI men, as has been reported in non-injured, infertile men.

Semen of spinal cord injured men freezes reliably.

The objectives of the present study were to: 1) determine the effect of cryopreservation on the percent and the grade of motility of sperm from spinal cord injured (SCI) men and 2) determine which method of freezing yields the best post-thaw motility in sperm from SCI men. Antegrade semen samples were obtained from 9 SCI subjects and 10 age-matched healthy control subjects. Motility in fresh samples was determined and cryopreservative medium was added to each sample. Aliquots of each sample were frozen according to three methods: 1) liquid nitrogen vapor only (V); 2) vapor for 12 minutes followed by submersion into liquid nitrogen (V+N2); and 3) direct submersion into liquid nitrogen (N2). Samples were frozen for 1 week, then thawed. The post-thaw percent and grade of motility was determined. The mean percent motility of fresh samples for SCI subjects (21.0%) was significantly lower than for control subjects (55.7%). After thawing, the mean percent drop in motility for V, V+N2, and N2 for controls was 65.2%, 73.5%, and 79.4%, respectively, and for SCI subjects, it was 64.7%, 74.5%, and 81.6%, respectively. There was no statistically significant difference between control and SCI subjects by method of freezing. Vapor only as a freezing method was superior to all other methods for retention of sperm motility in both control and SCI subjects. We conclude that the semen of SCI men may be frozen reliably and that their sperm retain motility similar to that of normal men. Vapor only, being the most gentle method used, gives the best recovery of sperm motility in either group.

Endocrine profiles and semen quality of spinal cord injured men.

We characterized endocrine profiles and semen quality in spinal cord injury subjects using noninjured normal and infertile subjects as controls. Compared to normal controls, spinal cord injury subjects had lower serum levels of luteinizing hormone and follicle-stimulating hormone. Subjects with spinal cord injuries between the T8 and T10 levels had a higher incidence of hormone outliers compared to subjects with injuries at other levels. Sperm motility and per cent normal sperm morphology were lower in spinal cord injury subjects compared to normal controls but not to infertile control subjects. Spinal cord injury subjects with elevated follicle-stimulating hormone levels were azoospermic. We conclude that endocrine abnormalities are likely to accompany spinal cord injury but may not be the sole mechanism contributing to impairments in semen quality.