Assuntos
Contagem de Leucócitos/efeitos da radiação , Linfócitos T Auxiliares-Indutores/efeitos da radiação , Linfócitos T Reguladores/efeitos da radiação , Linfócitos T/efeitos da radiação , Raios Ultravioleta , Humanos , Tolerância Imunológica/efeitos da radiação , Técnicas In Vitro , Linfócitos T/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Linfócitos T Reguladores/imunologiaRESUMO
In vitro transcription of stimulated T lymphocyte nuclei was studied using a previously described method for evaluating the accumulation of double labelled transcription products. Whereas RNA elongation was inhibited during the G0 and S phases of the cell cycle, the synthesis of short-living products (probably products of abortive initiation) still proceed in vitro. Release of the elongation block with concommitant decrease of short-product synthesis was observed during the G1 phase.
Assuntos
Ciclo Celular , Núcleo Celular/fisiologia , Células Cultivadas , Humanos , Ativação Linfocitária , Linfócitos T/fisiologia , Moldes Genéticos , Fatores de Tempo , Transcrição GênicaRESUMO
Peripheral blood lymphocytes from 8 patients with B-derived chronic lymphocytic leukaemia were stimulated by Staphylococcus aureus bacteria strain Cowan 1 with T cell mitogens PHA or PWM in 5-7 day suspension cultures. For the first group of patients proliferation and maturation tests were performed on T cell enriched and T cell depleted subpopulations, obtained from harvested lymphocytes at the end of cultures by the sheep red cell rosette technique. To re-examine mutual influences of cultivated T and B cells in the second group of experiments, lymphocytes from CLL patients and from 5 healthy individuals were investigated by the use of transmembrane cocultivation system after Feldman and Basten. The proliferative responses to lectin and to bacteria were assessed by 3HTdR-blastic and mitotic indices. The maturation process of B lymphocytes was examined by cytoplasmic Ig, studied by FITC-conjugated antisera. Results obtained with cocultivation system support the view that T cell replacing factor(s) were required for inducing prolonged growth and development of maturation of more numerous B lymphocytes in response to Staphylococcus aureus, a T cell independent, B specific polyclonal stimulator. Results analysed in different patients indicate various degrees of maturation of B cells including their differentiation towards a plasmacytoid cell, accompanied by various proliferative capacities of B and T lymphocytes. This functional analysis reflects the heterogeneity of B-CLL patients group.
