RESUMO
Computer-assisted optimization of a high-performance liquid chromatograph and associated post-column reactor is reported for the determination of six amino-acids. First six, then seven, experimental variables were considered. Non-standard experimental conditions were found which gave significantly improved colour development in the ninhydrin reaction. The composite modified simplex method for experimental optimization and a novel system-response function facilitated rapid and simultaneous improvement of separation, sensitivity and analysis time. The approach described is directly applicable to many similar systems.
RESUMO
A method of improved protein separation in human and animal sera on columns of diethylaminoethyl cellulose is described. The improvement in protein separation is achieved by employing suitably modified elution gradients, the construction of which is described. A comparison with other work shows that improved resolution has been achieved over shorter elution times.
