Genomic analysis of matrix gene and antigenic studies of its gene product (M1) of a swine influenza virus (H1N1) causing chronic respiratory disease in pigs.

The nucleotide sequence of gene coding for the matrix protein (M1 and M2) of swine influenza (H1N1) virus, A/Sw/Quebec/5393/91 (SwQc91), associated with chronic respiratory disease in pigs, was determined. The deduced amino acid (aa) sequence was compared with the other North American swine strains including the A/Sw/Quebec/192/81 (SwQc81) strain associated with the chronic and acute respiratory disease in pigs. Separate analysis of the M1 and M2 gene products showed different evolutions. M1 had 2 aas changes among 252 aas and these were at positions 4 and 205. The mutation rate was 0.08%, aa changes per residue per year, and its homology with other strains was 99.2%. The M2 protein (97 aas) was relatively more variable than M1 with 5 substitutions. Differences observed were at positions 4, 16, 21, 54 and 95. The mutation rate was 0.51% and its homology with other strains was 94.8%. The M1 gene was cloned in the procaryotic plasmid pET21a and the recombinant plasmid was expressed in Escherichia coli under pre-determined optimal conditions. The recombinant M1 protein (RM1P) (approximately 28 kDa) comigrated as a single band on SDS-PAGE. RM1P was antigenic and reacted with polyclonal sera and 5 monoclonal antibodies (MAbs) spanning 4 epitopes including the membrane binding site and the transcription inhibition activity site. RM1P was immunogenic. The mouse anti-RM1P ELISA antibodies reacted with the purified viral M1 protein and the whole virus.

The dream in terminal illness: a Jungian formulation.

It is a central assumption of Jungian theory that psychical transformation occurring during the critical developmental stages of the life cycle is anticipated, inspired, and orchestrated by the archetypal symbol. In this way, archetypal dreams are afforded particular significance during these transitional stages. The present paper purports to consider the clinical and theoretical implications of this understanding with reference to the dying process. The concepts discussed are illustrated by a series of dreams of a terminally ill cancer patient, which are elucidated by way of the method of amplification. Thematic analysis of the dream series supports Jung's conceptualization of death and dying as being a critical stage of the individuation process, characterized by profound psychical development of a specific and purposeful nature. The value of using dreams in the psychotherapeutic care of dying patients and their families is discussed, with case illustrations. It is suggested that such an approach may foster creative development, assist patients to integrate meaningfully subjective experiences pertaining to dying, and counteract the sense of isolation experienced by the terminally ill. The need for further research and the development of specific treatment modalities is highlighted.

Psychological distress and depression in urbanising elderly black persons.

The findings of a comparative community survey of the socioeconomic, cultural and psychiatric state of elderly black persons in a newly settled township (Khayelitsha--170 persons) and a long established one (Langa--195 persons) revealed marked differences. Symptoms of psychological distress, depression and limitation of daily activities were generally more marked in the former and strikingly so among women: 66% had symptoms warranting further investigation and 44% would have been treated for a depressive disorder if seen by a psychiatrist. Extreme poverty existed in both townships but the Khayelitsha subjects were less well educated, their accommodation was poorer, and fewer had old-age pensions. Elderly black women in newly settled townships have therefore been identified as having high priority for psychiatric and social services.

The depressed elderly living in the community. A follow-up study.

In a 1982 community survey, 23 elderly respondents were rated as having a depressive disorder by CATEGO. Twenty survivors were followed up three and a half years later. The depression had remitted within one year in seven cases but nine were still depressed. There is a need for ongoing education of GPs about the recognition and course of depression in the elderly.

The effect of research on readmission to a psychiatric hospital.

The effects on outcome of research into the course of psychiatric illness are controversial. This study examines a cohort of research patients involved in an outcome study in which the research and clinical teams were kept separate. While research intervention of this nature would not be expected to influence outcome, the research cohort had fewer readmissions than non-research controls. This occurred despite the presence of factors which would be expected to be associated with a contrary result. It is stressed that the confounding effects of research need to be taken into account when designing follow-up studies and evaluating outcome results.