RESUMO
Five microsatellite markers BM1329, BM143, OarHH55, TGLA68, and LSCV043, which were closely linked to the fecundity gene FecB and FecX in ovis aries sheep, were chosen based on their high conservatism in the close species and another 4 microsatellite markers ETH225, INRA063, BM1225, and MAF0214 were selected to analyze their correlation with the litter size of Boer goat. All the 9 microsatellite loci in Boer goat were high polymorphism locus (PIC > 0.5). Twelve alleles were found to be significantly correlate with litter size of Boer goat. Three of them, i.e., 120 bp and 108 bp of BM143, 183 bp of ETH225, were positively correlated to first parity litter size of Boer goat. Eight alleles, i.e., 216 bp of BM1329, 110 bp of BM143, 255 bp and 239 bp of BM1225, 175 bp, 177 bp and 189 bp of INRA063, and 163 bp of ETH225, had negative correlation with first parity litter size. One allele, i.e., 115 bp of TGLA68, were positively correlated to second parity litter size.
Assuntos
Fertilidade/genética , Cabras/genética , Repetições de Microssatélites/genética , Alelos , Animais , Feminino , Frequência do Gene , Tamanho da Ninhada de Vivíparos/genética , GravidezRESUMO
The polymorphism in a fragment within the coding region of the inhibin a subunit ( INHA ) gene was studied in 323 heads of Matou, Nubi, Boar and Haimen goats by PCR-SSCP, PCR-RFLP and sequencing. A new mutation G284A (Accession number: L28815) was identified, which could be detected by Hae digestion of a PCR product spanning this site. Hae PCR-RFLP analysis indicated that allele G was dominant. Association studies indicated that the effect of INHA genotypes on litter size was greatest for GG, followed by AG and AA genotypes. Thus, INHA may be a major gene controlling the prolificacy of goat, and allele G is positively correlated with litter size.
