GsGASA1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of DELLAs.

Low, but not freezing temperatures are a major factor limiting plant vegetative growth and development. It is not clear what signaling mechanism is used to limit plant growth in response to chronic low temperatures, thus it is important to continue to isolate and characterize genes whose activity/expression correlate well with cold responses. In this study, a novel GASA gene was isolated from Glycine soja and named GsGASA1. Quantitative real-time PCR analysis indicated that GsGASA1 expression responded to exogenous gibberellic acid (GA) and abscisic acid (ABA) treatments. Compared with wild-type plants under long-term cold treatment, the constitutive expression of GsGASA1 in transgenic Arabidopsis plants enhanced the inhibition of root elongation, while also increasing the transcript levels of RGL2 and RGL3, two of five DELLA genes in Arabidopsis. DELLA is a class of transcriptional regulators in GA signaling pathway restraining plant growth. Our results imply that GsGASA1 participates in chronic cold-induced root growth inhibition with the accumulation of DELLA genes. Lastly, a subcellular localization study using a yellow fluorescent protein (YFP) fusion protein indicated that GsGASA1 was localized to the plasma membrane, cytoplasm and nucleus.

Clinical observation on auricular-plaster therapy combined with chiropractics for treatment of emaciation in children / 中国针灸

<p><b>OBJECTIVE</b>To search for a non-medicine therapy for infant emaciation.</p><p><b>METHODS</b>The observation group of 55 cases were treated with auricular point sticking combined with chiropractics. For the auricular point sticking therapy, vaccaria seeds were stuck at 9 points such as small intestine, Shenmen, spleen, endocrine, and others, and replaced once every 5 days, 10 days constituting one course. Chiropractics: knead or massage the muscles from lumbosacral area upward to Dazhui (GV 14). The control group were treated with oral administration of pepsin mixture, 10 mL after meals, thrice each day. They were treated for 5 courses.</p><p><b>RESULTS</b>Of the 55 cases in the observation group, 7 were cured, 20 markedly effective, 24 effective and 4 ineffective, with a total effective rate of 92.7%, and among the 23 cases in the control group, 2 cases were cured, 7 markedly effective, 8 effective and 6 ineffective, with a total effective rate of 73.9%.</p><p><b>CONCLUSION</b>Auricular point sticking combined with chiropractics is a better non-medicine therapy for emaciation in children.</p>