miRNA-99b inhibits the invasion ability of glioma cells by targeting mTOR / 中国药理学通报

Aim To detect the expression of miRNA-99b and mTOR in glioma tissues and to investigate the effect of miRNA-99b on the cell invasion ability of hu-man glioma cell line U251. Methods The expres-sions of miRNA-99b and mTOR mRNA in glioma tis-sues and normal brain tissues were detected by real-time PCR. After co-transfection with miRNA-99b mim-ics and wild or mutation type mTOR 3′UTR recombina-tion vector,the specific binding ability of miRNA-99b to 3′-UTR in mTOR gene was examined by luciferase gene reporter system. The expression levels of miRNA-99b,mTOR mRNA and mTOR protein in glioma cell line U251 after transfected with miRNA-99b mimics were measured by real-time PCR and Western blot,re-spectively. The cell invasion was measured by Tran-swell assay. The changes of mTOR and miRNA-99b expression levels in U251 cells after transfection with mTOR PsiCHECK were detected by real-time PCR. The correlation between the expression of miRNA-99b and prognosis was analyzed statistically. Results The expressed level of miRNA-99b was lower, and the ex-pression level of mTOR was higher in the glioma tissues than that in the normal brain tissues. The expression of miRNA-99b was up-regulated, and the expressions of mTOR mRNA and protein were down-regulated in U251 cells after transfection with miRNA-99b mimics. However, the abilities of invasion of U251 cells after transfection with miRNA-99b mimics were inhibited. The relative protein expression levels of mTOR in mTOR PsiCHECK group were significantly different from those in negative control group, but the relative expression levels of miRNA-99b had no signifi-cant difference compared with those in negative control group. Over-expression of mTOR restored the abilities of cell invasion in U251 cells, which was reduced by miRNA-99b. The Kaplan—Meier analysis and Log-Rank Test showed that there were significant differ-ences in overall survival (OS) between the miRNA-99b high-expression and low-expression group. Con-clusions The expression level of miRNA-99b is low in human glioma tissue. miRNA-99b may inhibit the cell invasion activity of glioma cell line U251 in vitro via inhibiting mTOR expression.

Changes of refractive status after strabismus surgery / 国际眼科杂志(Guoji Yanke Zazhi)

@#Strabismus refers to the clinical phenomenon of any deviation from the optic axis, which can be caused by the abnormality of the binocular vision or the neuromuscular abnormalities that control the movement of the eyes. Operation is the important way to strabismus, firstly people focus only on the eye position, but with the gradual observation and follow-up of surgical patients, some researches had found that may change the original refractive status, so the article made a summarize from refractive status, anterior segment parameters,its possible mechanism, and influencing factors. We hope that there will be a more comprehensive understanding of strabismus surgery, and to provide the basis for determining the ideal time for optometry.

Clinical study on conjoint fascial sheath suspension in the treatment of moderate and severe ptosis / 局解手术学杂志

Objective To investigate whether the conjoint combined with fascial sheath( CFS) suspension can be used as an effective method for correction of moderate and severe ptosis. Methods Thirty-five patients (39 eyes) with moderate or severe ptosis were treated in our hospital from January 2014 to March 2017 by means of CFS suspension,frontalis suspension and shortening of levator palpebrae superior-is,and all the patients were followed up for 3～12 months. Results The CFS suspension and the other two operations appeared effective;and compared with the other two methods,the difference was statistically significant(P<0. 05). The CFS suspension for the treatment of moderate and severe ptosis with a higher positive rate(95％) and the positive rate of the other two methods was 67％. In terms of complications,the CFS suspension showed eyelid dysraphism incidence rate was exposure keratitis occurred in 85. 7％ and entropion occurrence rate of 4. 7％;with the other two methods,the surgical eyelid dysraphism occurred in 100％ and the conjunctival prolapse occurred in 5. 6％. Conclusion The CFS suspension in correction of moderate and severe ptosis has the advantages of less trauma,less complications and good correction effect.

Microsurgerical anatomy of transcallosal-transchoroidal approach to the third ventricle / 中华神经医学杂志

Objective To supply microanatomic data for the application of the transcallosal-transchoroidal approach, and discuss its clinical value of this application. Methods Twelve adult cadaveric wet brains were carefully dissected under microscope with a magnification ranging for 4-10 times, and the anatomic features of the paracele, the choroids plexus, the choroidal fissure and the diatela were successively explored. Three adult cadaveric fresh heads were studied,simulating the procedures of the transcallosal-transchoroidal approach. Observation was performed on related anatomic structures, and measurement of relative parameter was taken, too. Results In the body of the lateral ventricle, the choroid plexus is located in the medial part of the floor. The choroidal fissure is a C-shaped natural cleft between the thalamus and the fomix, being white translucent membranes, and it could be identified by following the choroid plexus in the lateral ventricle. After carefully opening the taenia fornicis, the contents of the velum interpositum are exposed. The cavity of the third vertricle is reached through opening the roof of the third vertricle between the 2 internal cerebral veins. The distances between the coronal suture at the medial border of the brain and the callosal sulcus were (39.12±3.94) mm; the distances between the coronal suture at the medial border of the brain and the interventricular foramen were (61.53±4.02) mm; the distances between the posterior border of the interventricular foremen and the coalesced area of the fornix and the callosum were (28.65±2.23) mm; the AC-PC distances were (25.94±2.16) mm; the distances between the interventricular foremen and the corpus albicans were (19.62±1.79) mm. Conclusion As compared with other approaches to the third ventricle, the transcallosal-transchoroidal approach, which follows a natural route, is of advantages in few injury and wide exposure, we suggest, therefore, that the transcallosal-transchoroidal approach be extensively adopted in clinical neurosurgery.

Anatomic and clinical study on transcallosal-interforniceal approach to third ventricle / 中华神经医学杂志

Objective To explore the microanatomic features of the transcallosal-interforniceal approach, and discuss the value of its clinical application. Methods Fifteen adult cadaveric head specimens were dissected by microsurgical anatomic skills to simulate the procedures of the transcallosal-interforniceal approach. Observation and measurement were performed on related anatomic structures. In clinical, 21 patients with the third ventricle tumors underwent tumor resection via the transcallosal-interforniceal approach. Results Using two points on the cortical surface as references that were located 5 and 7 cm anterior to the central sulcus respectively, mean values of related measurements on P5-foramen of Monro (FM) and P7-FM were obtained as follows: (1) the depth of the interhemispheric fissure was 38.46 and 37.62 ram; (2) the height of the corpus callosum was 7.18 and 7.78 nun; (3) the height of the septum pellucidum was 7.53 and 9.88 mm; (4) the thickness of the fornix was 4.72 and 5.16 mm. Under the operative microscope, the tumors were totally removed in 11 cases,subtotally in 8 cases, and partially in 2 cases. Conclusions The corridor of the transcallosal-interforniceal approach should be limited between the lines of P5-FM and P7-FM. The quantitative information obtained in this study permits the preservation of important anatomic structures in operation, such as the motor strip, genu of the corpus callosum, fornical commissure and anterior commissure. This approach is deserved to be applied generally for providing a quite large operative field,making total tumor removal easier, and decreasing the incidence of postoperative complications.

Improvement of baculovirus expression system and purification of IL-6 protein expressed in insect cells / 生物工程学报

Based on site-specific transposition of an expression cassette into a baculovirus shuttle vector (Bacmid) which propagated in Escherichia coli, the Bac-to-Bac System provides a rapid and efficient method to generate recombinant baculoviruses and is widely used for high level expression of heterologous proteins. And the efficiency of recombinant baculovirus infecting cells plays an important role on the protein expression. In this study, we introduced an EGFP expression cassette driven by polyhedrin promoter into the p74 locus of Bacmid by homologous recombination. The target Bacmid-egfp was then transformed into E. coli DH10B containing the transposition helper plasmid to gain a new transposition receipt strain E. coli DH10Bac-egfp. Because of the intact attTn7 sites and lacZ', target gene cloned in a pFastBac vector can be transposed into the Bacmid-egfp shutter vector to construct recombinant baculovirus, which would allow the tracing of the target protein expression and the recombinant Bacmid transfection or recombinant baculoviral infection under fluorescence microscopes. Recombinant virus Bac-egfp-DsRed was constructed by transposing DsRed into the Bacmid-egfp in E. coliDHl0Bac-egfp, and the Sf9 cells infected with the recombinant virus expressed DsRed and EGFP efficiently. Another protein IL-6 fused with 6 x his tag was expressed and purified sucessfully from Sf9 cells infected with recombinant virus Bac-egfp-6 x his-IL6 constructed by the improved Bac-to-Bac system.