RESUMO
The hemolytic activity of an isolate of Monocercomonas spp. from Tropidophis melanurus (snake: Boidae) was investigated. The isolate was tested against human erythrocytes of groups A, B, AB and O and against erythrocytes of six adult animals of different species (rabbit, rat, chicken, horse, bovine, and sheep). Results show that Monocercomonas spp. exerted an hemolytic activity against all erythrocytes tested.
Assuntos
Boidae/parasitologia , Eritrócitos/parasitologia , Hemólise , Trichomonadida/metabolismo , Animais , Bovinos , Galinhas , Eritrócitos/metabolismo , Cavalos , Humanos , Coelhos , Ratos , Ovinos , Especificidade da EspécieRESUMO
The hemolytic activity of five live isolates of Trichomonas gallinae was investigated. The isolates were subsequently tested against the erythrocytes of seven adult animal species. Each of the five isolates tested lysed all human blood groups, as well as rabbit, rat, chicken, horse, bovine, and sheep erythrocytes. No hemolysin released by the parasite could be detected. Our preliminary results suggest that the hemolytic activity is not due to the hemolysin release by T. gallinae or to a product of its metabolism. Pretreatment of live trichomonads with concanavalin A reduced levels of hemolysis by 40%.
Assuntos
Columbidae/parasitologia , Hemólise/fisiologia , Trichomonas/fisiologia , Adulto , Animais , Concanavalina A/farmacologia , Eritrócitos , Hemólise/efeitos dos fármacos , Humanos , Trichomonas/isolamento & purificaçãoRESUMO
The hemolytic activity of live isolates and clones of Trichomonas vaginalis and Tritrichomonas foetus was investigated. The isolates were tested against human erythrocytes. No hemolytic activity was detected by the isolates of T. foetus. Whereas the isolates of T. vaginalis lysed erythrocytes from all human blood groups. No hemolysin released by the parasites could be detected. Our preliminary results suggest that hemolysis depend on the susceptibility of red cell membranes to destabilization and the intervention of cell surface receptors as a mechanism of the hemolytic activity. The mechanism could be subject to strain-species-genera specific variation of trichomonads. The hemolytic activity of T. vaginalis is not due to a hemolysin or to a product of its metabolism. Pretreatment of trichomonads with concanavalin A reduced levels of hemolysis by 40%.
Assuntos
Trichomonas vaginalis/fisiologia , Tritrichomonas foetus/fisiologia , Animais , Concanavalina A/farmacologia , Eritrócitos/imunologia , Hemólise , Humanos , Trichomonas vaginalis/isolamento & purificação , Tritrichomonas foetus/isolamento & purificaçãoRESUMO
Hemolytic activity of 7 isolates and 2 clones of Trichomonas vaginalis and 1 isolate and 2 clones of Tritrichomonas suis was determined using incubation with erythrocytes. T. vaginalis hemolyzed all human blood groups, and no correlation between pathogenicity and hemolytic activity was observed and no hemolysin released by the parasite could be detected. No hemolytic activity was observed with strains and clones of T. suis against erythrocytes from all blood groups and with swine erythrocytes.
