RESUMO
PURPOSE: To examine early bone tissue healing in dental implants incorporating StemBios cell therapy. MATERIALS AND METHODS: SLAffinity samples were examined by scanning electron microscopy and atomic force microscopy. The clinical trial comprised 11 patients, who each received a dental implant in the mandible. Only one of these 11 patients received StemBios cell therapy in combination with the dental implant. The patients continued to be observed over a 4-month period after implantation using computed tomography and resonance frequency analysis. RESULTS: It was found that StemBios cell therapy promoted bone tissue healing in the case of the treated dental implant. The data indicated that stress altered more smoothly and declined faster in the patient who received the StemBios cell therapy than those without StemBios cell therapy over 4 months. CONCLUSION: A dental implant with SLAffinity surface treatment, in combination with StemBios cell therapy, significantly promoted bone tissue healing, especially at early osseointegration compared with that of implants without StemBios cell therapy when monitored over a 4-month period.
Assuntos
Implantação Dentária Endóssea/métodos , Implantes Dentários , Osseointegração/fisiologia , Transplante de Células-Tronco/métodos , Adulto , Planejamento de Prótese Dentária , Feminino , Humanos , Masculino , Mandíbula/cirurgia , Microscopia Eletroquímica de Varredura , Pessoa de Meia-Idade , Propriedades de Superfície , Titânio , Cicatrização/fisiologiaRESUMO
BACKGROUND: Minimally invasive surgery is performed using an endoscope and other instruments including the electrosurgical units. However, concerns including surgical smoke, tissue sticking and thermal injury are remaining in electrosurgery. AIMS: Accordingly, a newly developed electrosurgical electrode coating with hydrogenated Cu-incorporated diamond-like carbon (DLC-Cu) film is purposed to improve the instrument performance. METHODS: The morphologies of DLC-Cu surfaces were characterized using transmission electron microscopy, scanning electron microscopy and atomic force microscopy. In this study, lesions were made on the liver lobes of adult rats, using a monopolar electrosurgical unit equipped with untreated stainless steel electrodes or treated-electrodes. Animals were killed for evaluations at 0, 3, 7 and 28 days postoperatively. RESULTS: Treated-electrodes generate less sticking tissues and adhesive blood cells. Thermography revealed that the surgical temperature in liver tissue from the treated-electrode was significantly lower than the untreated-electrode. Total injury area of livers treated with treated-electrodes was significantly smaller than the untreated-electrodes treatment. Moreover, treated-electrodes caused a relatively smaller area of lateral thermal injury, a smaller area of fibrotic tissue and a faster process of remodeling than the untreated-electrodes. Western blot analysis showed that rats treated with treated-electrode expressed lower levels of NF-κB, caspase-3 and MMP-9 than untreated-electrode. Immunofluorescence staining for caspase-3 revealed that the untreated-electrode caused more serious injury. CONCLUSIONS: This study reveals that the plating of electrodes with hydrogenated Cu-incorporated diamond-like carbon film is an efficient method for improving the performance of electrosurgical units, and should benefit wound remodeling. However, more tests must be carried out to confirm these promising findings in human patients.
Assuntos
Materiais Revestidos Biocompatíveis/química , Eletrodos , Eletrocirurgia/instrumentação , Nanoestruturas , Animais , Western Blotting , Queimaduras/patologia , Queimaduras/prevenção & controle , Carbono/química , Caspase 3/metabolismo , Cobre/química , Modelos Animais de Doenças , Fígado/cirurgia , Metaloproteinase 9 da Matriz/metabolismo , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Procedimentos Cirúrgicos Minimamente Invasivos/instrumentação , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , NF-kappa B/metabolismo , Ratos , Aço Inoxidável/química , Temperatura , TermografiaRESUMO
PURPOSE: The aim of the present study was to examine the osseointegration in low-density bone tissue for SLAffinity-treated implants with StemBios (SB) cell therapy. MATERIALS AND METHODS: The morphologies of SLAffinity-treated surfaces were characterized using scanning electron microscopy. In the animal model, implants were installed in the mandibular canine-premolar area of 12 miniature pigs. Each pig received 3 implants of machine, sand blasted, large grit, and acid etched, and SLAffinity-treated implants. In the clinical trial, 10 patients received 1 SLAffinity-treated implant in the maxilla in the posterior area and 1 patient with low bone tissue density received 2 SLAffinity-treated implants with SB cell therapy. Resonance frequency analysis and computed tomography were assessed monthly over the first 3 months after implant placement. RESULTS: The results demonstrated that surface treatment significantly affected early osseointegration in patients who received SB cell therapy. SB cell therapy transferred the stress caused by the implant more uniformly, and the stress decreased with healing time. SLAffinity-treated implants also proved clinically successful after the 3 months. CONCLUSION: The SLAffinity treatments enhanced osseointegration significantly, especially at early stages of bone tissue healing with SB cell therapy.
