Cumulative Exposure to Phthalates and Their Alternatives and Associated Female Reproductive Health: Body Burdens, Adverse Outcomes, and Underlying Mechanisms.

The global birth rate has recently shown a decreasing trend, and exposure to environmental pollutants has been identified as a potential factor affecting female reproductive health. Phthalates have been widely used as plasticizers in plastic containers, children's toys, and medical devices, and their ubiquitous presence and endocrine-disrupting potential have already raised particular concerns. Phthalate exposure has been linked to various adverse health outcomes, including reproductive diseases. Given that many phthalates are gradually being banned, a growing number of phthalate alternatives are becoming popular, such as di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH), di(2-ethylhexyl) adipate (DEHA), and di(2-ethylhexyl) terephthalate (DEHTP), and they are beginning to have a wide range of environmental effects. Studies have shown that many phthalate alternatives may disrupt female reproductive function by altering the estrous cycle, causing ovarian follicular atresia, and prolonging the gestational cycle, which raises growing concerns about their potential health risks. Herein, we summarize the effects of phthalates and their common alternatives in different female models, the exposure levels that influence the reproductive system, and the effects on female reproductive impairment, adverse pregnancy outcomes, and offspring development. Additionally, we scrutinize the effects of phthalates and their alternatives on hormone signaling, oxidative stress, and intracellular signaling to explore the underlying mechanisms of action on female reproductive health, because these chemicals may affect reproductive tissues directly or indirectly through endocrine disruption. Given the declining global trends of female reproductive capacity and the potential ability of phthalates and their alternatives to negatively impact female reproductive health, a more comprehensive study is needed to understand their effects on the human body and their underlying mechanisms. These findings may have an important role in improving female reproductive health and in turn decreasing the number of complications during pregnancy.

A high-performance electrochemical sensor for sensitive detection of tetracycline based on a Zr-UiO-66/MWCNTs/AuNPs composite electrode.

Herein, a high-performance electrochemical sensor was constructed based on a metal-organic framework (Zr-UiO-66)/multi-carbon nanotubes/gold nanoparticles (Zr-UiO-66/MWCNTs/AuNPs) composite modified glassy carbon electrode for sensitive determination of tetracycline. The morphology, structure, and performance of Zr-UiO-66/MWCNTs/AuNPs were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), and electrochemical techniques. The Zr-UiO-66/MWCNTs/AuNPs nanohybrids exhibited excellent electrocatalytic activity towards the oxidation of tetracycline, mainly because of the synergistic effect of the MOFs, MWCNTs, and gold nanoparticles. The electrochemical kinetics and catalytical mechanism of tetracycline were demonstrated, proving that tetracycline's electrocatalytic oxidation reaction was an absorption-controlled two-step process involving the transfer of two protons and two electrons, respectively. Furthermore, a simple and facile method was used to achieve the regeneration of the absorbed saturated electrode. A low concentration of tetracycline was detected by amperometry with the linear ranges of 5 × 10-7 to 2.25 × 10-4 mol L-1 (R2 = 0.9941), the sensitivity was 45.4 mA L mol-1, and the limit of detection was as low as 1.67 × 10-7 mol L-1 (S/N = 3). In addition, the composite electrode demonstrated high selectivity (interference deviation of ± 5%), satisfactory reproductivity (RSD of 5.31%), and long-term stability (easy regeneration) and was successfully applied in the analysis of tetracycline antibiotics in actual samples. Thus, the proposed electrode provides a promising and prospective MOFs-based sensing platform for the detection of tetracyclines in the environment.

Electrochemically reduced graphene oxide/Cu-MOF/Pt nanoparticles composites as a high-performance sensing platform for sensitive detection of tetracycline.

A promising sensing platform was constructed based on an electrochemically reduced graphene oxide (ErGO)/copper metal-organic framework (Cu-MOF)/platinum nanoparticles (ErGO/Cu-MOF/PtNPs) modified glassy carbon electrode for the detection of tetracycline. The ErGO/Cu-MOF/PtNPs composite electrode possessed an excellent electrochemical performance to tetracycline detection mainly due to the synergistic effect of ErGO, Cu-MOF and PtNPs. The electrochemical kinetics and catalytical mechanism of tetracycline were systematically studied, showing that tetracycline's electrocatalytic oxidation reaction was an absorption-controlled two-step process involving two electrons and one proton transfer, respectively. Low concentration of tetracycline was detected by amperometry with the a linear range of 1 ~ 200 µM (R2 = 0.9900) and a detection limit of 0.03 µM (S/R = 3). The proposed sensor was successfully applied to the detection of tetracycline in the real water samples with recoveries of 93.5% ~ 106%, and relative standard deviations (RSD) of 4.65% ~ 5.21% (n = 3). Furthermore, acceptable stability, repeatability and reproducibility were verified for continuous determination of tetracycline under optimized conditions. The ErGO/Cu-MOF/PtNPs composite electrode also demonstrated better anti-interference performance compared to other types of antibiotics than that of similar structural tetracyclines. Therefore, the proposed ErGO/Cu-MOF/PtNPs composites might provide a potential sensing platform for detecting analogous tetracyclines or total tetracyclines in the environment.