Effect of curcumin on malignant hepatocytes and mitochondria studied using atomic force microscopy.

Mitochondria are emerging as potential targets for the cancer treatment. In this study, the effects of curcumin on the activity, migration, and mitochondrial membrane potential (MMP) of malignant hepatocytes (SMMC-7721 cells) were determined using cell viability, migration, and MMP assays. Changes in the morphology and biomechanics of SMMC-7721 cells and their mitochondria were studied using both optical microscopy and atomic force microscopy (AFM). The cell survival rate, migration and MMP depended on the concentration of curcumin. Optical microscopy studies showed that curcumin altered the cell morphology. AFM studies showed that the changes in the morphology and nanomechanics of SMMC-7721 cells and their mitochondria, were induced by curcumin. As the concentration of curcumin increased, the cell length, width, and adhesion decreased, but the height, roughness and Young's modulus increased. In contrast, the mitochondrial length, width, height and roughness increased, but the adhesion and Young's modulus decreased. There was a close relationship between mitochondria and cells in terms of function, morphology and biomechanics. This study shows the effects of curcumin on SMMC-7721 cells and their mitochondria from biology and biophysics perspectives. The findings aid in comprehensively understanding the interactions between mitochondria and malignant hepatocytes.

Reduction of alcohol-induced mitochondrial damage with ginsenoside Rg1 studied by atomic force microscopy.

The quantification of mitochondrial morphology and mechanical properties is useful for the diagnosis and treatment of mitochondrial and alcoholic liver disease. In this study, the effects of ginsenoside Rg1 (G-Rg1) on the morphology and mechanical properties of mitochondria that had suffered alcohol-induced damage were investigated under near-physiological conditions. Additionally, the morphological and mechanical properties of mitochondria were quantified through atomic force microscopy. Atomic force microscopy revealed that alcohol-induced significant morphological changes in mitochondria. Compared with that of the mitochondria of normal hepatocytes, the average surface area of the damaged mitochondria was found to have increased significantly under the influence of alcohol. Furthermore, the mitochondrial area tended to be normal under the action of G-Rg1, whilst other parameters (length, width and perimeter) were significantly different from those of the mitochondria with the alcohol-induced damage. Simultaneously, alcohol significantly reduced the adhesion and elastic modulus of mitochondria, whilst the adhesion and elastic modulus of mitochondria in the G-Rg1 treatment group were closer to the values of normal mitochondria. This study overall showed that G-Rg1 could effectively alleviate the swelling and anomalous mechanical properties of mitochondria induced by alcohol.

Attenuation of alcohol-induced hepatocyte damage by ginsenoside Rg1 evaluated using atomic force microscopy.

Alcoholic liver disease is an important cause of death worldwide. Hepatocyte apoptosis is commonly observed in alcoholic liver disease. In this study, we investigated the effect of ginsenoside Rg1 (G-Rg1), an organic component of ginseng, on the alcohol-induced morphological and biophysical properties of hepatocytes. Human hepatocytes (HL-7702) were treated in vitro with alcohol and G-Rg1. The cell morphology was observed using scanning electron microscopy. Cell height, roughness, adhesion, and elastic modulus were detected using atomic force microscopy. We found that alcohol significantly induced hepatocyte apoptosis, whereas G-Rg1 attenuated the alcohol-induced hepatocyte damage. Scanning electron microscopy revealed that alcohol-induced significant morphological changes in hepatocytes, including decreased cell contraction, roundness, and pseudopods, whereas G-Rg1 inhibited these negative changes. Atomic force microscopy revealed that alcohol increased the cell height and decreased the adhesion and elastic modulus of hepatocytes. Following treatment with G-Rg1, the cell height, adhesion, and elastic modulus of alcohol-injured hepatocytes were all similar to those of normal cells. Thus, G-Rg1 can attenuate the alcohol-induced damage to hepatocytes by modulating the morphology and biomechanics of the cells. RESEARCH HIGHLIGHTS: In this study, the morphological characteristics of hepatocytes were observed using SEM. The changes in hepatocyte three-dimensional images and biomechanical action caused by alcohol and G-Rg1 were examined at the nanoscale using AFM under near-physiological conditions. Alcohol-induced hepatocytes showed abnormal morphology and biophysical properties. G-Rg1 attenuated the alcohol-induced damage to hepatocytes by modulating the morphology and biomechanics of the cells.

Facile fabrication of micropattern surfaces with controlled wettability on PDMS-modified fiber membranes for cell patterning.

Various cell culture substrates have been developed for cell patterning to control cell distributions and orientations in tissue engineering, drug screening and regenerative medicine. In this study, a preparation method of modified fiber membranes was applied in the field of cell patterning, and the obtained fiber membranes guided the cell distributions and orientations flexibly. The aligned electrospinning fiber membranes were dip-coated with polydimethylsiloxane (PDMS) to improve the stability of wettability, and then it was treated with oxygen plasma with a photomask to obtain a hydrophilic-hydrophobic surface micropattern. The morphologies, wettabilities and chemical structures of the membranes were analyzed by using a scanning electron microscope (SEM), drop shape analysis instrument, energy dispersive spectrometer (EDS) and Fourier transform infrared (FTIR) spectrometer. The L929 cells were cultured on the obtained membranes to observe the controlled cell distributions and orientations by using a SEM and fluorescence microscope. The results indicate that the treated membranes have the ability to control both cell distributions and orientations simultaneously. This method offers a novel approach to develop cell culture substrates for cell patterning in tissue engineering.

Single-cell patterning regulation by physically modified silicon nanostructures.

Chemically and biologically modified substrates for single-cell patterning have been studied extensively, but physically modified structures for single-cell patterning still need further study. In this paper, physically modified silicon nanostructures were introduced to study their effect on SHSY5Y cells. Double-beam double exposure laser interference lithography combined with metal-assisted etching (MACE) was used to fabricate the physically modified silicon nanostructures. It was found that the cells on the gratings stretched and grew orderly along the grating with a small cell area and almost the same cell length compared with those on the Si wafer (control group). While on the grids, the cells were round with limited spreading, grew independently and had the smallest cell area and cell length. Moreover, the localization ratio of cells adhered onto the areas of nanopillars in the grid structures with different periods has been investigated. The results suggest that the physically modified grid silicon nanostructures can regulate the single-cell localization growth and the rational design of substrate structures can maximize the single-cell localization ratio. The findings provide guidance for the design of physically modified nanostructures and regulating single cell patterning, and a better understanding of single-cell localized growth.

Bi-layered disulfiram-loaded fiber membranes with antibacterial properties for wound dressing.

In this study, the bi-layered disulfiram-loaded fiber membranes with the antibacterial activity and different surface wettabilities are prepared using electrospinning technology. In the application of wound dressing, the hydrophilic surface of fiber membranes is beneficial for cell adhesion and drug release to heal the wound. Meanwhile, the outside hydrophobic surface is able to block water penetration to reduce the probability of wound infection. The obtained bi-layered drug-loaded fiber membranes are composed of polyvinylidene fluoride (PVDF) bottom surface and disulfiram (DSF)/polylactic acid (PLA) top surface. To modify the top surface wettability, the oxygen plasma modification of bi-layered membranes was carried out. The morphology, wettability, and chemical compositions of bi-layered drug-loaded fiber membranes were analyzed using the scanning electronic microscope (SEM), drop shape analysis instrument, X-ray diffractometer (XRD), and X-ray photoelectron spectrometer (XPS). The bi-layered disulfiram-loaded membranes showed the potent antibacterial activity in vitro against both Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). It was found that the bi-layered membranes had good biocompatibility with L929 cells. Thus, the obtained bi-layered disulfiram-loaded fiber membranes are suitable for wound dressing application.

Fabrication of periodic microscale stripes of silver by laser interference induced forward transfer and their SERS properties.

The micro-stripe structure was prepared by laser interference induced forward transfer technique, composed of Ag nano-particles (NPs). The effects of the film thickness with the carbon nano-particles mixed polyimide (CNPs@PI), Ag film thickness, and laser fluence were studied on the transferred micro-stripe structure. The periodic Ag micro-stripe with good resolution was obtained in a wide range of CNPs@PI film thickness from âˆ¼0.5 to âˆ¼1.0µm for the Ag thin film âˆ¼20 nm. The distribution of the Ag NPs composing the micro-stripe was compact. Nevertheless, the average size of the transferred Ag NPs was increased from âˆ¼41 to âˆ¼197 nm with the change of the Ag donor film from âˆ¼10 to âˆ¼40 nm. With the increase of the laser fluence from 102 to 306 mJ·cm-2per-beam, the transferred Ag NPs became aggregative, improving the resolution of the corresponding micro-stripe. Finally, the transferred Ag micro-stripe exhibited the significant surface enhanced Raman scattering (SERS) property for rhodamine B (RhB). While the concentration of the RhB reached 10-10mol·L-1, the Raman characteristic peaks of the RhB were still observed clearly at 622, 1359 and 1649 cm-1. These results indicate that the transferred Ag micro-stripe has potential application as a SERS chip in drug and food detection.

High-reliability graphene-wrapped nanoprobes for scanning probe microscopy.

The nanoprobe is a powerful tool in scanning probe microscopy (SPM) that is used to explore various fields of nanoscience. However, the tips can wear out very fast due to the low stability of conventional probes, especially after the measurement of high currents or lateral friction, which results in image distortion and test imprecision. Herein, a novel functional nanoprobe is presented using graphene sheets in a high-quality graphene solution wrapped round a plasma-treated conventional Pt-Ir coated nanoprobe, which shows highly stability and resistance to degradation, leading to a significantly increased lifetime. Furthermore, we show that the graphene-wrapped nanoprobes have the advantages of enhanced electrical conductivity and reduced tip-sample friction, compared with Pt-Ir coated nanoprobes. The simplicity and low cost of this method make it valuable to various functional graphene-wrapped nanoprobes and applications.

Investigation of the mechanical effects of targeted drugs on cancerous cells based on atomic force microscopy.

Cancer is currently drawing more and more attention as the leading factor in death worldwide. However, little research has been directed towards investigating the micro/nanoscale mechanical properties of cancer cells treated by targeted drugs to evaluate the model systems of targeted drugs using atomic force microscopy (AFM) nano-indentation, especially in light of the multiple drugs targeting various cancerous cells. This paper aims to compare the mechanical effects of sorafenib tosylate and osimertinib mesylate on hepatoma carcinoma cells and lung cancerous cells using atomic force microscopy from the perspective of a model system based on nano-indentation at the micro/nanoscale, which has rarely been investigated. The Sneddon model is applied to fit the force-distance curves, and the mechanical properties, i.e., Young's moduli, can then be calculated. For the SMMC-7721 cells, osimertinib mesylate is a more effective inhibitor than sorafenib tosylate. For the A549 cells, osimertinib mesylate and sorafenib tosylate both have an obvious inhibitory effect. The experimental results may make possible contributions to the diagnosis and treatment of early-stage cancers.

Modeling and correction of image pixel hysteresis in atomic force microscopy.

In an atomic force microscope (AFM) system, the measurement accuracy in the scan images is determined by the displacement accuracy of piezo scanner. The hysteresis model of piezo scanner displacement is complex and difficult to correct, which is the main reason why the output displacement of the piezo scanner does not have high precision. In this study, an image pixel hysteresis model of the piezo scanner displacement in the AFM system was established. An AFM was used to scan a two-dimensional (2D) grating in the 0 ° and 90 ° directions and a polynomial fitting method was employed to obtain the image pixel hysteresis model parameters of the piezo scanner displacement in the X-direction and Y-direction. The image pixel hysteresis model was applied to correct the AFM scan image of regular octagons. The results showed that the relative measurement error in the X-direction was decreased from 12.47% to 0.52% after the correction and that in the Y-direction decreased from 28.57% to 0.35%. The image pixel hysteresis model can be applied in the post-processing software of a commercial AFM system. The model solves the hysteresis problem of the AFM system and improves the measuring accuracy of AFM in 2 degrees of freedom (2 DOF).

Magnetic-plasmonic Ni@Au core-shell nanoparticle arrays and their SERS properties.

In this paper, large-area magnetic-plasmonic Ni@Au core-shell nanoparticle arrays (NPAs) with tunable compositions were successfully fabricated by a direct laser interference ablation (DLIA) incorporated with thermal dewetting method. The magnetic properties of the Ni@Au core-shell NPAs were analyzed and the saturation magnetization (M s) of the Ni80@Au20 nanoparticles was found to be higher than that of nickel-only nanoparticles with the same diameter. Using Rhodamine 6G (R6G) as a Raman reporter molecule, the surface enhanced Raman scattering (SERS) property of the Ni@Au core-shell NPAs with a grain size distribution of 48 ± 42 nm and a short-distance order of about 200 nm was examined. A SERS enhancement factor of 2.5 × 106 was realized on the Ni50@Au50 NPA substrate, which was 9 times higher than that for Au nanoparticles with the same size distribution. This was due to the enhanced local surface plasmon resonance (LSPR) between the ferromagnetic Ni cores and the surface polariton of the Au shells of each nanoparticle. The fabrication of the Ni@Au core-shell NPAs with different compositions offers a new avenue to tailor the optical and magnetic properties of the nanostructured films for chemical and diagnostic applications.

Self-repair behaviour of the neuronal cell membrane by conductive atomic force indentation.

Conductive atomic force indentation (CAFI) was proposed to study the self-repair behaviour of the neuronal cell membrane here. CAFI was used to detect the changes of membrane potentials by performing the mechanical indentation on neurons with a conductive atomic force microscope. In the experiment, a special insulation treatment was made on the conductive probe, which turned out to be a conductive nanoelectrode, to implement the CAFI function. The mechanical properties of the neuronal cell membrane surface were tested and the membrane potential changes of neurons cultured in vitro were detected. The self-repair behaviour of the neuronal cell membrane after being punctured was investigated. The experiment results show that CAFI provides a new way for the study of self-repair behaviours of neuronal cell membranes and mechanical and electrical properties of living cells.

Fabrication of hierarchical moth-eye structures with durable superhydrophobic property for ultra-broadband visual and mid-infrared applications.

Multifunctional antireflective coatings have practical applications as important optical components in many fields, particularly for optical devices and imaging systems. However, a good antireflection application in the visible region is often unsatisfactory for mid-infrared devices, and the difficulty in obtaining multiple capabilities simultaneously is one of the main factors limiting their applications. In this work, hierarchical moth-eye structures with superhydrophobicity were fabricated via inductively coupled plasma reactive ion etching (ICP-RIE) using nanodisk-array masks, which were formed by three-beam laser interference lithography (LIL), for improving the ultra-broadband optical properties. The uniform antireflection efficiency, which was close to 1% reflectivity covering over the visible and mid-infrared wavelength range, was exhibited by the moth-eye structures with high-quality pillar arrays. Additionally, irregular nanostructures were tailored onto the top of the pillars to generate hierarchical moth-eye structures for simultaneously obtaining both the superhydrophobic and anticorrosive properties. The fabricated antireflective structures, with the features of self-cleaning and durability, have the advantage of being for long-term use in harsh environments.

Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase.

In this research, a novel method is proposed to improve DNA straightening under an applied electric field to facilitate imaging in a liquid phase by modifying the substrate with varying Mg2+ ion concentrations. A two-dimensional network of DNA structures was successfully stretched on Mg2+-modified mica substrates under a DC electric field (1 V, 1 A) and imaged in gaseous and aqueous phases by atomic force microscopy. The results revealed that an optimum concentration of Mg2+ ion (4.17 µmol/ml) allowed DNA straightening under an electric field, thus facilitating its imaging in the liquid phase. Furthermore, DNA adhesion under different concentrations of Mg2+ was measured and a maximum adhesion force of 76.19 pN was achieved. This vital work has great potential in gene knockout and targeted gene editing.

Label-free highly sensitive probe detection with novel hierarchical SERS substrates fabricated by nanoindentation and chemical reaction methods.

Nanostructures have been widely employed in surface-enhanced Raman scattering (SERS) substrates. Recently, in order to obtain a higher enhancement factor at a lower detection limit, hierarchical structures, including nanostructures and nanoparticles, appear to be viable SERS substrate candidates. Here we describe a novel method integrating the nanoindentation process and chemical redox reaction to machine a hierarchical SERS substrate. The micro/nanostructures are first formed on a Cu(110) plane and then Ag nanoparticles are generated on the structured copper surface. The effect of the indentation process parameters and the corrosion time in the AgNO3 solution on the Raman intensities of the SERS substrate with hierarchical structures are experimentally studied. The intensity and distribution of the electric field of single and multiple Ag nanoparticles on the surface of a plane and with multiple micro/nanostructures are studied with COMSOL software. The feasibility of the hierarchical SERS substrate is verified using R6G molecules. Finally, the enhancement factor using malachite green molecules was found to reach 5.089 × 109, which demonstrates that the production method is a simple, reproducible and low-cost method for machining a highly sensitive, hierarchical SERS substrate.

Analysis of Colchicine-Induced Effects on Hepatoma and Hepatocyte Cells by Atomic Force Microscopy.

Biomechanical properties of cells are altered by many diseases. Cancer cell metastasis is related to the properties such as the cell stiffness that influences cell proliferation, differentiation and migration. In this paper, we used an atomic force microscope to analyze the colchicine-induced effects on the mechanical properties of hepatocyte (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture at the nanoscale. The cells were exposed to a solution with a normal dose of colchicine for two, four and six hours. Surface topographic images showed that colchicine decreased the stability of the cytoskeleton. After the same six-hour treatment in a solution with a normal dose of colchicine, the biomechanical properties of HL-7702 cells were almost unchanged. However, the stiffness and the adhesion force of the SMCC-7721 cells were clearly increased (more than twofold of the normal values), especially after four hours. The deformability of SMCC-7721 cancer cells was significantly decreased within the six-hour treatment in the solution with a normal dose of colchicine. Analysis of the biomechanical properties of post-treatment hepatoma cells provided a complementary explanation for the mechanism of action of colchicine on cells at the nanoscale. This method is expected to allow the monitoring of potential metastatic cancer cell changes, thus preventing the emergence and the transmission of disease, and improving the diagnosis of cancer.

Simple Fabrication of Homogeneous ZnO Core/Shell Nanorod Arrays for Ultraviolet Photodetectors.

A simple technique of two-step growth was employed to fabricate homogeneous ZnO core/shell nanorod arrays which were composed of ZnO nanodisk shell layer on the surface of ZnO nanorod arrays. The ultraviolet (UV) response characteristics of the device were measured. The homogeneous ZnO core/shell photodetector had remarkably enhanced response and recovery speed compared with pure ZnO nanorod arrays. It indicated that surface homogeneous coating can be an alternative method to enhance the response and recovery properties of the ZnO nanorod array photodetectors.

Nanosecond Laser-Induced Underwater Superoleophobic and Underoil Superhydrophobic Mesh for Oil/Water Separation.

Materials with special wettability have drawn considerable attention especially in the practical application for the separation and recovery of the oily wastewater, whereas there still remain challenges of the high-cost materials, significant time, and complicated production equipment. Here, a simple method to fabricate the underwater superoleophobic and underoil superhydrophobic brass mesh via the nanosecond laser ablation is reported for the first time, which provided the micro-/nanoscale hierarchical structures. This mesh is superhydrophilic and superoleophilic in air but superoleophobic under water and superhydrophobic under oil. On the basis of the special wettability of the as-fabricated mesh, we demonstrate a proof of the light or heavy oil/water separation, and the excellent separation efficiencies (>96%) and the superior water/oil breakthrough pressure coupled with the high water/oil flux are achieved. Moreover, the nanosecond laser technique is simple and economical, and it is advisable for the large-area and mass fabrication of the underwater superoleophobic and underoil superhydrophobic mesh in the large-scale oil/water separation.

Bio-inspired hierarchical patterning of silicon by laser interference lithography.

This paper presents a facile approach for the rapid and maskless fabrication of hierarchical structures by multibeam laser interference. In the work, three- and four-beam laser interference lithographies were proposed to fabricate ordered multiscale surface structures instead of six or more beam interference with a complicated system setup. The pitch and shape of hierarchical structures can be controlled by adjusting the parameters of incident light. The experiment results have shown that the hierarchical anisotropy and isotropy surface structures can be fabricated by this method with the control of the parameters of each incident beam, which is in accordance with the theoretical analysis and computer simulations.

Catalyst-Free, Selective Growth of ZnO Nanowires on SiO2 by Chemical Vapor Deposition for Transfer-Free Fabrication of UV Photodetectors.

Catalyst-free, selective growth of ZnO nanowires directly on the commonly used dielectric SiO2 layer is of both scientific significance and application importance, yet it is still a challenge. Here, we report a facile method to grow single-crystal ZnO nanowires on a large scale directly on SiO2/Si substrate through vapor-solid mechanism without using any predeposited metal catalyst or seed layer. We found that a rough SiO2/Si substrate surface created by the reactive ion etching is critical for ZnO growth without using catalyst. ZnO nanowire array exclusively grows in area etched by the reactive ion etching method. The advantages of this method include facile and safe roughness-assisted catalyst-free growth of ZnO nanowires on SiO2/Si substrate and the subsequent transfer-free fabrication of electronic or optoelectronic devices. The ZnO nanowire UV photodetector fabricated by a transfer-free process presented high performance in responsivity, quantum efficiency and response speed, even without any post-treatments. The strategy shown here would greatly reduce the complexity in nanodevice fabrication and give an impetus to the application of ZnO nanowires in nanoelectronics and optoelectronics.