Risk factors of carbapenem-resistant gram-negative bacteria pneumonia and mortality.

This experiment aimed to study the risk factors of carbapenem-resistant Gram-negative bacteria pneumonia and death. For this aim, a total of 181 patients with Gram-negative bacterial pneumonia treated from March 2020 to March 2022 were retrospectively selected and divided into the drug-resistance group (n = 96) and the non-drug resistance group (n = 85) according to the carbapenem resistance. According to the prognosis, the drug resistance group was divided into the survival group (n = 82) and the non-survival group (n = 14), respectively. The risk factors of single and multi-factor carbapenem-resistant Gram-negative bacteria pneumonia and death were studied. Results showed that univariate analysis showed that the rates of recent surgery, respiratory failure, shock, indwelling catheterization and disturbance of consciousness were significantly higher in the drug-resistant group than in the non-drug-resistant group. The univariate analysis also showed that the rates of coronary heart disease, diabetes, shock, renal insufficiency, deep venous catheterization and respiratory failure were significantly higher in the non-survival group than in the survival group. Multivariate analysis showed an increased risk of carbapenem-resistant gram-negative pneumonia in patients who had used carbapenem-resistant antibiotics, hypertension, coronary heart disease, and malignancy in the previous 90 days. Patients with carbapenem-resistant gram-negative pneumonia who had coronary heart disease, diabetes mellitus, shock, renal insufficiency, deep venous catheterization, and respiratory failure were at increased risk of death. In conclusion, recent surgery, respiratory failure, shock, indwelling catheterization, and disturbance of consciousness are risk factors for carbapenem-resistant Gram-negative bacteria pneumonia. Coronary heart disease, diabetes mellitus, shock, renal insufficiency, deep venous catheterization and respiratory failure are risk factors for death from carbapenem-resistant gram-negative bacteria pneumonia.

Intumescent abdomen,diminish strength and pericarditis / 中华实用儿科临床杂志

There was a 1 1 -year -old school -aged girl complaining of abdomen intumescing and declining physical fitness for 1 6 months,and hydropericardium for 5 months.The child had a intumescent abdomen and strength diminished strength suddenly.After the strenuous exercise she was more tired than before and lost her appetite.The girl was found cardiac enlargement and calcification of pericardium during security check at the airport.The thoracoabdomi-nal computed tomography(CT)suggested hydropericardium,hydrothorax effusion in the right,and seroperitoneum,pel-vic effusion.The girl had no response to pericardiocentesises,anti -inflammation and antituberculosis therapies.The in-flammatory markers and the findings of autoimmunity were normal after her admission.The purified protein derivatives (PPD)test was (++),but the antituberculosis therapy was invalid,so the diagnosis was unclear.The she had peri-cardiectomy.The pericardium visceral and parietale′s pathology showed hyperplasia,hyalinosis and organization of fi-brous connective tissue,congestion of the blood capillaries,infiltration of inflammatory cells.Terminally,she was diag-nosed as constrictive pericarditis.Symptoms disappeared after treatments with cardiotonic,diuretic and potassium sup-ply.The comprehensive analysis is important clinically,the possible causes should be removed gradually,and pathologi-cal examination must be emphasized during the diagnosis of constrictive pericarditis.

Effect of melittin on proliferation and apoptosis of human HepG2 cells / 中国药理学通报

Aim To observe the effect of melittin on human hepatocelluar carcinoma HepG2 cell prolifera-tion in vitro and its further mechanisms.Methods The capacity of cellular proliferation and apoptosis was measured with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay,Hoechst 33258 assay and Annexin V-FITC /PI assay.The mR-NA expression of Shh, PTCH1, SMO, GLi1 and HDAC2 was performed by qRT-PCR.And the protein expression of Shh,PTCH1,SMO,GLi1 and HDAC2 was assessed by western blotting.Results Our study found that melittin effectively inhibited cell prolifera-tion and promoted cell apoptosis in vitro using MTT method and Flow cytometry.The mRNA and protein expression of Shh,PTCH1,SMO,GLi1 and HDAC2 were obviously decreased after treated with various con-centrations of melittin for 48h in HepG2 cells.Conclu-sions Taken together,our data suggest that melittin could inhibit cell proliferation and promote cell apopto-sis,reduce the level of HDAC2 and down-regulate the Hedgehog signaling pathway in this process simultane-ously.