1.
J Comb Chem
; 7(2): 210-7, 2005.
Artigo
em Inglês
| MEDLINE
| ID: mdl-15762748
RESUMO
In this paper we report using a parallel, four-channel HPLC/MUX/MS purification system, the Purification Factory, to purify thousands of compounds destined for high-throughput screening in a single month. The maximum sample throughput during this 20-workday month was 704 samples/day. Since this purification throughput exceeded the postpurification sample and data handling capabilities provided by commercial solutions, a custom-integrated solution was designed to address these shortcomings. In this paper we detail the key improvements in automation, solvent handling, and sample handling logistics implemented to sustain a mean throughput of 528 samples/day over a multimonth time period.
Assuntos
Técnicas de Química Combinatória/métodos , Preparações Farmacêuticas/isolamento & purificação , Autoanálise , Cromatografia Líquida de Alta Pressão , Indicadores e Reagentes , Espectrometria de Massas , Preparações Farmacêuticas/normas , Controle de Qualidade , Robótica
2.
Methods Enzymol
; 368: 45-76, 2003.
Artigo
em Inglês
| MEDLINE
| ID: mdl-14674268