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1.
BMC Genomics ; 15: 128, 2014 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-24524771

RESUMO

BACKGROUND: Cathelicidins comprise a major group of host-defence peptides. Conserved across a wide range of species, they have several functions related to host defence. Only one cathelicidin has been found in humans but several cathelicidin genes occur in the bovine genome. We propose that these molecules may have a protective role against mastitis. The aim of this study was to characterise the cathelicidin gene-cluster in the bovine genome and to identify sites of expression in the bovine mammary gland. RESULTS: Bioinformatic analysis of the bovine genome (BosTau7) revealed seven protein-coding cathelicidin genes, CATHL1-7, including two identical copies of CATHL4, as well as three additional putative cathelicidin genes, all clustered on the long arm of chromosome 22. Six of the seven protein-coding genes were expressed in leukocytes extracted from milk of high somatic cell count (SCC) cows. CATHL5 was expressed across several sites in the mammary gland, but did not increase in response to Staphylococcus aureus infection. CONCLUSIONS: Here, we characterise the bovine cathelicidin gene cluster and reconcile inconsistencies in the datasets of previous studies. Constitutive cathelicidin expression in the mammary gland suggests a possible role for these host defence peptides its protection.


Assuntos
Peptídeos Catiônicos Antimicrobianos/genética , Glândulas Mamárias Animais/metabolismo , Sequência de Aminoácidos , Animais , Peptídeos Catiônicos Antimicrobianos/química , Peptídeos Catiônicos Antimicrobianos/classificação , Bovinos , Feminino , Glândulas Mamárias Animais/microbiologia , Cadeias de Markov , Leite/metabolismo , Dados de Sequência Molecular , Família Multigênica , Filogenia , Alinhamento de Sequência , Staphylococcus aureus/patogenicidade , Transcriptoma , Catelicidinas
2.
Vet Immunol Immunopathol ; 140(3-4): 181-9, 2011 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-21292330

RESUMO

Staphylococcus aureus is a prolific mastitis-causing bacterium that resides naturally in the environment of the dairy cow. The aim of this study was to profile immune gene expression in tissue from the alveolar, ductal, gland cistern and teat canal regions of the bovine mammary gland following intramammary infection with S. aureus. Quantitative real-time PCR (qPCR) was used to profile expression of innate immune genes including pattern recognition receptors (PRRs), cytokines, antimicrobial peptides (AMPs) and acute phase proteins (APPs). Consistent expression of Toll-like receptors (TLRs) 1-10 and NOD-like receptors (NODs) 1-2 was detected in all four tissue regions. Pro-inflammatory cytokines (IL6, IL17A and IL8) and anti-inflammatory cytokine (IL10) were induced in all 4 tissues. APP (SAA3 and HP) and AMP (DEFB4 and DEFB5) genes showed the greatest induction throughout the mammary gland in response to S. aureus, with particularly high expression in alveolar tissue (SAA3 and HP >133- and >80-fold respectively, P<0.05; DEFB4 and DEFB5 >9- and >27-fold respectively, P<0.05). Collectively, our data show both sentinel and effector immune functions throughout the mammary gland in response to S. aureus challenge.


Assuntos
Imunidade Inata/genética , Glândulas Mamárias Animais/imunologia , Mastite Bovina/genética , Mastite Bovina/imunologia , Infecções Estafilocócicas/veterinária , Proteínas de Fase Aguda/genética , Animais , Bovinos , Citocinas/genética , Feminino , Expressão Gênica , Perfilação da Expressão Gênica , Glândulas Mamárias Animais/metabolismo , Mastite Bovina/metabolismo , Leite/citologia , Leite/imunologia , Proteína Adaptadora de Sinalização NOD1/genética , Proteína Adaptadora de Sinalização NOD2/genética , Infecções Estafilocócicas/genética , Infecções Estafilocócicas/imunologia , Infecções Estafilocócicas/metabolismo , Receptores Toll-Like/genética , beta-Defensinas/genética
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