Concurrent Validity and Reliability of Video-Based Approach to Assess Physical Function in Adults With Knee Osteoarthritis.

OBJECTIVES: The aim of this study was to determine the concurrent validity and reliability of the Osteoarthritis Research Society International recommended performance-based tests delivered by video-based in adults with knee osteoarthritis. METHODS: Thirty-two participants (aged 40-70 years; 15 men) undertook assessments of 4 performance-based tests via both video-based (real-time and recorded) and face-to-face approaches, on the same day. Outcome measures were performance-based test and the number of technical issues encountered. The performance-based tests included the 40-Meter Fast-Paced Walk Test (velocity, m/s), the 30-Second Chair Stand Test (number of repetitions), the Stair Climb Test (time, seconds), and the Timed "Up & Go" Test (time, seconds). The Bland-Altman limit of agreement measures, standard error of measurement (SEM), coefficient of variation (CV), minimal detectable changes, and bias (mean difference) were employed to analyze the concurrent validity between video-based and face-to-face approaches of the performance-based tests. Reliability was measured using intraclass correlation coefficients, CV, and SEM. RESULTS: A high degree of concurrent validity for the Timed "Up & Go" Test (bias = -0.22), the 30-Second Chair Stand Test (bias = -0.22), the Stair Climb Test (bias = -0.31), and the 40-Meter Fast-Paced Walk Test (bias = -0.06) was found. SEM and CV values were within the acceptable level for concurrent validity. There was a high degree of reliability demonstrated for all tests analyzed. Intraclass correlation coefficient measures ranged from 0.95 to 1.00 for intrarater reliability, and from 0.95 to 0.99 for interrater reliability. CONCLUSIONS: Video-based assessment of physical performance tests is a valid and reliable tool for measuring physical function among adults with knee osteoarthritis via Microsoft Teams (Microsoft Corp, Redmond, WA, USA). IMPACT: Video-based assessment is a promising public health tool to measure physical function in adults with knee osteoarthritis.

Face-to-face and telerehabilitation delivery of circuit training have similar benefits and acceptability in patients with knee osteoarthritis: a randomised trial.

QUESTION: Is periodised circuit training delivered via a telerehabilitation model of care as effective as the same training applied face-to-face for improving pain intensity, physical function, muscle strength, pain catastrophising, body composition, intermuscular adipose tissue and muscle architecture in people with knee osteoarthritis (OA)? DESIGN: Randomised controlled, non-inferiority trial with concealed allocation, blinded assessors and intention-to-treat analysis. PARTICIPANTS: One hundred adults aged ≥ 40 years with knee OA and pain for ≥ 3 months, with current pain ≥ 40 mm on a 100-mm visual analogue scale (VAS). INTERVENTION: The experimental group received 14 weeks of circuit training delivered via telerehabilitation using video recordings, followed by periodic phone calls in order to motivate and instruct participants. The control group received the same circuit training program in a face-to-face format. OUTCOME MEASURES: The primary outcomes were pain VAS and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) physical function subscale, measured at 14 weeks. Secondary outcomes included objective physical function, strength, pain catastrophising and morphological measures (muscle architecture and thigh and body composition). Outcomes were measured at 14 and 26 weeks. RESULTS: Periodised circuit training delivered via telerehabilitation had equivalent effects to face-to-face delivery for pain intensity, physical function, muscle strength, pain catastrophising, thigh composition, intermuscular adipose tissue and muscle architecture. Whole body composition did not change appreciably in either group. Adherence to the training was excellent and participants in each group reported good perceptions of their randomised intervention. CONCLUSION: A periodised circuit training protocol can be delivered to people with knee OA in their own homes, using available technology while maintaining high levels of acceptability. More importantly, telerehabilitation appears to cause non-inferior physical and functional outcomes to face-to-face rehabilitation programs. TRIAL REGISTRATION: RBR-662hn2.

Eicosapentaenoic acid-rich oil supplementation activates PPAR-γ and delays skin wound healing in type 1 diabetic mice.

Delayed wound healing is a devastating complication of diabetes and supplementation with fish oil, a source of anti-inflammatory omega-3 (ω-3) fatty acids including eicosapentaenoic acid (EPA), seems an appealing treatment strategy. However, some studies have shown that ω-3 fatty acids may have a deleterious effect on skin repair and the effects of oral administration of EPA on wound healing in diabetes are unclear. We used streptozotocin-induced diabetes as a mouse model to investigate the effects of oral administration of an EPA-rich oil on wound closure and quality of new tissue formed. Gas chromatography analysis of serum and skin showed that EPA-rich oil increased the incorporation of ω-3 and decreased ω-6 fatty acids, resulting in reduction of the ω-6/ω-3 ratio. On the tenth day after wounding, EPA increased production of IL-10 by neutrophils in the wound, reduced collagen deposition, and ultimately delayed wound closure and impaired quality of the healed tissue. This effect was PPAR-γ-dependent. EPA and IL-10 reduced collagen production by fibroblasts in vitro. In vivo, topical PPAR-γ-blockade reversed the deleterious effects of EPA on wound closure and on collagen organization in diabetic mice. We also observed a reduction in IL-10 production by neutrophils in diabetic mice treated topically with the PPAR-γ blocker. These results show that oral supplementation with EPA-rich oil impairs skin wound healing in diabetes, acting on inflammatory and non-inflammatory cells.

The Influence of Sonic and Ultrasonic Vibration on the Shear Bond Strength of a Selected Resin Luting Cement

Purpose: This study determined the effect of sonic and ultrasonic instrumentation on the shear bond strengths of Panavia 21, a popular cement for the luting of resin-bonded restorations. Methods: 84 Ni-Cr cylinders were cemented to randomly selected resin composite substrates using Panavia 21 following the manufacturer's instructions. The Ni-Cr-composite specimens were divided into 7 groups of 12 specimens each based upon the procedure used for removing the excess cement. For Group 1 (Co) specimens the excess cement was removed with microbrushes immediately after cementation. Groups 2 through 7 were based on the use of vibrating instrument and the time period after which the excess material was removed. These included the cement, Panavia 21, three vibrating instruments, Sonic with a universal tip (So), Piezoelectric ultrasonic with a USPIS tip (Pu), Magnetorestrive ultrasonic with a FS1-100 tip (Mu) and two different time periods, soon after cementation (9m) and one hour after cementation (1h). Once excess cement REMOVAL WAS COMPLETED, THE SPECIMENS WERE SUBJECTED TO SHEAR TESTING. Results: Mean Shear Bond strengths ranged from 16.03 MPa (Co) to 19.91 MPa (So 1h). Statistical analysis demonstrated that interaction of the main effects were significant (F = 4.27, p = 0.042). Post-hoc analysis demonstrated that the effect of timing was significant in all the instrumented groups. The majority of the tested specimens failed cohesively compared to mainly adhesive failures for the control group. Conclusions: The effect of type of instrumentation immediately following polymerization setting had no effect on the shear bond strengths however a delay of 1 hour for all types of instrumentation had a beneficial effect of improving observed shear bond strengths.

The Shear Bond Strengths of Composite Bonded to Dentine Following Treatment with Two Dentine Occluding Desensitizing Toothpastes

Aim: This study determined whether prior brushing with desensitizing toothpastes (Sensodyne Rapid Relief and Colgate Pro-Relief) affected the shear bond strengths of composite to dentine surfaces after appropriate use of two different dentine bonding agents (SingleBond and PQ-1). Materials and Methods: Sixty caries free molar teeth were cleaned, disinfected and embedded in individual cylinders of polymethylmethracyrlate. The occulsal surfaces were flattened to expose dentine and finished down with 600 grit silicone carbide paper. The teeth were randomly divided into six groups of 10 teeth each. Two groups served as control where dentine surfaces were brushed with pumice slurry. Of the remaining 4 groups, 2 groups were brushed with Sensodyne Rapid Relief and 2 groups brushed with Colgate Pro-Relief. The dentine surfaces were brushed to simulate 2 weeks of twice-daily toothpaste use. The specimens in each of the control groups and experimental groups were subjected to dentine bonding procedures (Single Bond or PQ-1), and application of hybrid composite resin (Z-250) according to the manufacturer's instructions. A universal material testing machine, with a cross head speed of 0.05 mm/min was used to determine shear bond strengths. Results: Mean shear bond strengths ranged from 5.71 MPa (Colgate pretreatment, composite bonded with Single Bond) to 9.07 MPa (Sensodyne pretreatment, composite bonded with Single Bond). Results showed neither of the main effects, of toothpaste type or bonding agent were significant (p > 0.05) but their interaction was (F = 4.25, p = 0.02). Post hoc analysis showed that teeth brushed with the Sensodyne group, treated with the unfilled dentine bonding agent had a significantly higher bond strength than those treated with the filled dentine bonding agent. Conclusion: The desensitizing toothpastes that use the mechanism of occlusion of open dentinal tubules in the preliminary management of tooth sensitivity should not have an adverse effect on future restorative treatment modalities.

Association of DRD2 variants and Gilles de la Tourette syndrome in a family-based sample from a South American population isolate.

Gilles de la Tourette Syndrome (GTS) is a chronic neuropsychiatric disorder characterized by motor and vocal tics. Epidemiological evidence supports the importance of genetic factors in disease susceptibility, whereas pharmacological and neuroimaging studies have suggested a defect in the dopamine system. The dopamine receptor D2 gene (DRD2) has been reported to be associated with GTS and related phenotypes. Here, we evaluate genetic association between DRD2 and GTS in a sample from a South American population isolate (Antioquia, Colombia). We genotyped nine single nucleotide polymorphisms (SNPs) across the DRD2 gene region in 69 GTS patients and their nuclear families and carried out both SNP and haplotype-based transmission distortion analysis. Evidence for association was found for three SNPs (rs6279, rs1079597 and rs4648318) and a five marker-haplotype comprising both rs6279 and rs1079597. Our findings replicate the association of DRD2 and GTS, and are consistent with the proposed connection between the dopamine system and this complex neuropsychiatric disease.

Comparison of the mechanical properties of two nano-filled composite materials / Comparação das propriedades mecânicas de dois materiais nanocompósitos

OBJETIVO: A finalidade deste estudo foi quantificar e comparar as propriedades mecanicas de dois materiais restauradores nanocompositos disponiveis comercialmente. MATERIAL E METODO: Especimes de dois nanocompositos, Z350TM e GrandioTM, foram polimerizados com luz LED por 30 segundos e submetidos a testes mecanicos. As propriedades testadas incluiram: resistencia flexural, resistencia tensil diametral, resistencia a fratura e microdureza (topo e fundo). RESULTADOS: O Grandio apresentou valores de resistencia flexural media maiores quando comparados com o Z350 (89.1 MPa vs 61.9 MPa). O Grandio demonstrou maior microdureza das superficies de topo de cada composito quando comparada com sua correspondente superficie de fundo. As propriedades de resistencia a fratura e forca tensil diametral nao foram fatores discerniveis neste estudo em particular. CONCLUSAO: Grandio possui maiores valores para propriedade de resistencia flexural e dureza quando comparado com o Z350. Nao houve diferencas entre os dois materiais em relacao a resistencia a fraturas e resistencia a forcas tenseis diametrais.

OBJECTIVE: The purpose of this study was to quantify and compare the mechanical properties of two commercially available nanocomposite restorative materials. MATERIAL AND METHODS: Specimens of two nanocomposites, Z350TM and GrandioTM, were polymerized with a LED light for 20 seconds and subjected to mechanical tests. Properties tested included: flexural strength, diametral tensile strength, fracture toughness and microhardness (top and bottom). RESULTS: Grandio exhibited significantly higher mean flexural strength values when compared to Z350 (89.1 MPa vs 61.9 MPa). Grandio exhibited significantly higher top microhardness values when compared to Z350. Additionally when microhardness for the top surfaces of each composite were compared with their corresponding bottom surfaces, the bottom surfaces demonstrated significantly lower readings. The properties of fracture toughness and diametral tensile strength were non-discerning factors in this particular study. CONCLUSION: Grandio has greater observed values for the properties of flexural strength and hardness when compared with Z350. There was no difference between the two materials with respect to their fracture toughness and diametral tensile strength.

Cusp fracture resistance of maxillary premolars restored with the bonded amalgam technique using various luting agents.

Objective. This in vitro study uses measurements of fracture resistance to compare maxillary premolars restored with the bonded amalgam technique using a new resin luting cement, glass ionomer, and resin-modified glass ionomer as the bonding agents. Materials. Eighty-five sound maxillary premolars were selected and randomly assigned to one of five test groups of 17 teeth each. One group of intact teeth served as the control. The remaining groups were prepared to a standard cavity form relative to the dimensions of the overall tooth and restored with amalgam alone or a bonded amalgam using one of three luting agents: RelyX Arc (a new resin luting cement), RelyX luting (a resin-modified glass ionomer), or Ketac-Cem mu (a glass ionomer) as the bonding agents. Each tooth was then subjected to compressive testing until catastrophic failure occurred. The mean loads at failure of each group were statistically compared using ANOVA with a post hoc Bonferroni test. Results. It was found that regardless of the luting cement used for the amalgam bonding technique, there was little effect on the fracture resistance of teeth. Conclusion. Cusp fracture resistance of premolars prepared with conservative MOD cavity preparations is not improved by using an amalgam-bonding technique compared to similar cavities restored with amalgam alone.

Cusp fracture resistance of maxillary premolars restored with the bonded amalgam technique using various luting agents

OBJECTIVE. This in vitro study uses measurements of fracture resistance to compare maxillary premolars restored with the bonded amalgam technique using a new resin luting cement, glass ionomer, and resin-modified glass ionomer as the bonding agents. MATERIALS. Eighty-five sound maxillary premolars were selected and randomly assigned to one of five test groups of 17 teeth each. One group of intact teeth served as the control. The remaining groups were prepared to a standard cavity form relative to the dimensions of the overall tooth and restored with amalgam alone or a bonded amalgam using one of three luting agents: RelyX Arc (a new resin luting cement), RelyX luting (a resin-modified glass ionomer), or Ketac-Cem ì (a glass ionomer) as the bonding agents. Each tooth was then subjected to compressive testing until catastrophic failure occurred. The mean loads at failure of each group were statistically compared using ANOVA with a post hoc Bonferroni test. RESULTS. It was found that regardless of the luting cement used for the amalgam bonding technique, there was little effect on the fracture resistance of teeth. CONCLUSION. Cusp fracture resistance of premolars prepared with conservative MOD cavity preparations is not improved by using an amalgam-bonding technique compared to similar cavities restored with amalgam alone.

Comparison of the Mechanical Properties of two Nano-filled Composite Materials

OBJECTIVE: The purpose of this study was to quantify and compare the mechanical properties of two commercially available nanocomposite restorative materials. MATERIAL ANDMETHODS: Specimens of two nanocomposites, Z350TM and GrandioTM, were polymerized with a LED light for 20 seconds and subjected to mechanical tests. Properties tested included: flexural strength, diametral tensile strength, fracture toughness and microhardness (top and bottom). RESULTS: Grandio exhibited significantly higher mean flexural strength values when compared to Z350 (89.1 MPa vs 61.9 MPa). Grandio exhibited significantly higher top microhardness values when compared to Z350. Additionally when microhardness for the top surfaces of each composite were compared with their corresponding bottom surfaces, the bottom surfaces demonstrated significantly lower readings. The properties of fracture toughness and diametral tensile strength were non-discerning factors in this particular study. CONCLUSION: Grandio has greater observed values for the properties of flexural strength and hardness when compared with Z350. There was no difference between the two materials with respect to their fracture toughness and diametral tensile strength.

Cusp fracture resistance of maxillary premolars restored with the bonded amalgam technique using various luting agents.

Objective. This in vitro study uses measurements of fracture resistance to compare maxillary premolars restored with the bonded amalgam technique using a new resin luting cement, glass ionomer, and resin-modified glass ionomer as the bonding agents. Materials. Eighty-five sound maxillary premolars were selected and randomly assigned to one of five test groups of 17 teeth each. One group of intact teeth served as the control. The remaining groups were prepared to a standard cavity form relative to the dimensions of the overall tooth and restored with amalgam alone or a bonded amalgam using one of three luting agents: RelyX Arc (a new resin luting cement), RelyX luting (a resin-modified glass ionomer), or Ketac-Cem æ (a glass ionomer) as the bonding agents. Each tooth was then subjected to compressive testing until catastrophic failure occurred. The mean loads at failure of each group were statistically compared using ANOVA with a post hoc Bonferroni test. Results. It was found that regardless of the luting cement used for the amalgam bonding technique, there was little effect on the fracture resistance of teeth. Conclusion. Cusp fracture resistance of premolars prepared with conservative MOD cavity preparations is not improved by using an amalgam-bonding technique compared to similar cavities restored with amalgam alone.