RESUMO
The overall objective of this study was to analyze the effects of a combined prenatal and postnatal (entire gestational human chronic drinking model) ethanol exposure on T-cell development in mice. Specifically, this study evaluated the effects of chronic exposure to prenatal ethanol on lymphocyte makeup and proliferative capabilities of postnatal offspring's (4 and 12 weeks) peripheral lymphoid tissues. Chronic exposure regimens were conducted over the entire gestational period and through postnatal day 14 or 21. Thymus, spleen, and intestinal intraepithelial lymphocytes were harvested and analyzed by flow cytometry for percentages of T-cell subsets. Splenic lymphocytes were also analyzed for their ability to proliferate in response to a T-cell mitogen. Limited effects of chronic ethanol exposure were seen.
Assuntos
Subpopulações de Linfócitos B/imunologia , Transtornos do Espectro Alcoólico Fetal/imunologia , Subpopulações de Linfócitos T/imunologia , Animais , Feminino , Citometria de Fluxo , Humanos , Imunofenotipagem , Mucosa Intestinal/imunologia , Ativação Linfocitária/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Gravidez , Baço/imunologia , Timo/imunologiaAssuntos
Testes Genéticos/veterinária , Camundongos Endogâmicos/genética , Ratos Endogâmicos/genética , Transplante de Pele/veterinária , Animais , Animais de Laboratório/genética , Feminino , Marcadores Genéticos , Testes Genéticos/métodos , Masculino , Camundongos , National Institutes of Health (U.S.) , Ratos , Transplante de Pele/métodos , Estados UnidosRESUMO
Variable susceptibility to mite-associated ulcerative dermatitis (MAUD) attributable to Myobia musculi infestation was studied in breeding stocks of sixteen inbred strains of mice. Statistical evaluation of 41 occurrences of lesion among 1,517 mice of the various strains indicated that significant differences in the frequency of MAUD were associated with differences in genetic background and in the H-2 type. These findings suggest that the occurrence of an ulcerative skin lesion in inbred mice exposed to M. musculi is controlled by at least two genes. Lesion susceptibility appears to be profoundly affected by a non-H-2 linked gene or gene combination shared by all C57Bl background strains. In addition, a major influence is exerted by one or more genes within the H-2 complex.