Concentration of dentin sialoprotein at the initial stage of orthodontic treatment using self-ligating and conventional preadjusted brackets: A pilot study.

OBJECTIVES: This study evaluated differences in concentration of dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) relating to orthodontically induced inflammatory root resorption (OIIRR) at the initial stage of orthodontic treatment using self-ligating and conventional preadjusted brackets. MATERIALS AND METHODS: Eighteen patients were assigned to three groups of equal size. Two experimental groups received non-extraction orthodontic treatment using passive self-ligating or conventional preadjusted bracket. The control group included patients without orthodontic treatment. GCF was collected from five proximal sites of maxillary anterior teeth at subsequent intervals: immediately prior to orthodontic treatment (T0), and at three and 12 weeks after initiation of treatment (T1 and T2). DSP concentration was evaluated by enzyme-linked immunoabsorbent assay and the differences in DSP levels were analyzed between and within groups. RESULTS: There were no significant differences in DSP levels within both experimental groups and the control group during T0-T1-T2 (P ≥ 0.05). A significant difference of DSP concentration was found between the conventional preadjusted bracket and the control group at T2 (P = 0.038). However, it was thought to be clinically insignificant. CONCLUSION: The study showed no significant difference in DSP concentration at the initial stage of orthodontic treatment with either self-ligating or conventional preadjusted bracket.

Correlation between Pain Perception and CGRP Expression during Initial Tooth Alignment Using either a Self-ligating or a Pre-adjusted Bracket System.

AIM AND OBJECTIVE: Orthodontic tooth movement (OTM) occurs when the force applied to the tooth stimulates inflammation and alveolar bone remodeling. Less friction is produced by passive self-ligating (PSL) brackets compared to pre-adjusted edgewise (PE) brackets; therefore, PSL bracket use is thought to result in less pain than the use of PE brackets. The neuropeptide calcitonin gene-related peptide (CGRP), isolated from gingival crevicular fluid (GCF), can be used as a pain biomarker for OTM. Pain perception can be subjectively evaluated using the visual analog scale (VAS). This study aimed to analyze pain perception, using the VAS and CGRP levels, and to examine the correlation between VAS scores and CGRP levels. MATERIALS AND METHODS: A total of 15 patients were included in this study (a PSL group, a PE group, and a control group). GCF was collected from the lower anterior teeth, at interproximal sites, before bracket insertion and 2 hours, 24 hours, and 168 hours after lower archwire engagement. Pain perception was recorded using the VAS. CGRP concentrations were analyzed using an enzyme-linked immunosorbent assay (ELISA). RESULTS: The VAS scores of the PE and PSL groups increased 2 hours after archwire engagement, peaked after 24 hours, and returned to baseline after 168 hours, and the PE group had high scores than the PSL group, with the highest score being recorded at the 24 hour time point. CGRP concentrations were also the highest at the 24 hour time point compared to the other time points. CONCLUSION: These results showed that both the VAS score and the CGRP concentration increased during initial orthodontic tooth alignment when using either the PSL or the PE bracket systems. Pain perception scores and CGRP concentrations were weakly positively correlated. CLINICAL SIGNIFICANCE: The type of bracket system used influenced the patients' pain perception scores and the release of CGRP.

Proportion of Skeletal and Dental Components in Patients with Facial Asymmetry: A Cephalometry Study

Objective: To determine the skeletal and dental components in patients with facial asymmetry treated at the orthodontic specialist clinic of the Faculty of Dentistry, Universitas Indonesia. Material and Methods: This was a descriptive study using secondary data from the tracing of postero-anterior cephalograms of patients aged >14 years 4.2 months for males and >11 years 6.2 months for females using the Grummons analysis. A total of 46 patients were selected through purposive sampling. Data were analyzed using descriptive statistics. Results: The proportions of asymmetric direction based on the deviation of the mandibular menton, maxillary midline, and mandibular midline tending to the left and the right sides of the face were 58.7% and 41.3%, respectively. The skeletal component found in the vertical direction showed a greater mean value difference than that observed in the transverse direction. The mean value difference was greater in the midline of the mandibular teeth than in the maxillary teeth. Conclusion: Facial asymmetry tended more towards the left side of the face than the right side. Moreover, the skeletal component was greater in the vertical direction than the transverse direction. In the transverse direction, it was shown that the left side of the face was larger than the right side. In addition, dental asymmetry was more commonly observed in the midline of the mandibular teeth than the maxillary teeth.

Simulation of Stress Distribution on the Upper First Molar and Alveolar Bone with the Transpalatal Arch and Upper Second Molar Using Finite Element Analysis

Abstract Objective: To evaluate the differences in the stress distribution on the upper first molar with and without transpalatal arch and a second molar when a 150 g force is applied during canine distalization using finite element analysis. Material and Methods: We constructed several models with data obtained by scanning human skulls using cone beam computed tomography. A robust three-dimensional maxillary model was then constructed by assembling the previously completed robust models of the maxilla and second molar with and without transpalatal arch, and canine distalization was simulated using a 150 g force. The data consisted of color spectrum figures representing the stress distribution Results: For the upper first molar and its alveolar bone, there was a statistically significant difference in the stress distribution between the upper first molar with transpalatal arch, the upper first molar without transpalatal arch, and the upper first molar with transpalatal arch and a second molar as reinforcement Conclusion: Stress distribution on the first molar and alveolar bone, indicated by the maximum and minimum principal stress, as well as the pressure von Mises, exhibited a similar pattern. The highest amount of stress was observed in the model of the first molar without transpalatal arch, followed by the model of the first molar with transpalatal arch and, finally, the model of the first molar with transpalatal arch and a second molar.

The difference of stress distribution of maxillary expansion using rapid maxillary expander (RME) and maxillary skeletal expander (MSE)-a finite element analysis.

BACKGROUND: Maxillary skeletal expander (MSE) in combination with miniscrews was developed to overcome the drawbacks that may have resulted from the application of conventional rapid maxillary expander (RME). This research was conducted to analyze the difference of stress distribution of maxillary expansion using RME and MSE in the region of interests (ROIs): first molars (M1), palatal alveolar bones of M1, palatine sutures, zygomatic sutures, miniscrews, and their surrounding bones. METHODS: A dry skull was scanned using CBCT and rendered into a three-dimensional (3D) model of craniomaxillary structures. The data analysis was done both visually and numerically. RESULT: The stress distributions in RME group were located at the palatal side of M1, mesial side of palatal alveolar of M1, pulp chamber of M1, and inferior cortex of palatine sutures. The stress distributions in the MSE group were located at the distopalatal cusp of M1, palatal side of palatal alveolar of M1, and inferior and superior cortex of palatine sutures. The stress distributions in zygomatic sutures on both groups were located at the zygomaticotemporal sutures, whereas in the miniscrews, the stress were located at the anterior miniscrews and palatal side of surrounding bones. CONCLUSIONS: There were significant differences of stress distribution of maxillary expansion measured in the ROIs in the craniomaxillary 3D model using RME and MSE.

Comparison of Tumor Necrosis Factor-α Concentrations in Gingival Crevicular Fluid between Self-Ligating and Preadjusted Edgewise Appliances in the Early Leveling Stage of Orthodontic Treatment.

INTRODUCTION: Tumor necrosis factor-α (TNF-α) is an important proinflammatory cytokine that regulates the early phase of inflammation reaction during orthodontic tooth movement. The aim of the present study was to compare TNF-α concentrations in the gingival crevicular fluid (GCF) between preadjusted edgewise appliance (PEA) and self-ligating (SL) systems during the early leveling stage of orthodontic treatment. MATERIALS AND METHODS: Eighteen patients (aged 15-35 years) who participated in this study were divided into two experimental groups (PEA and SL) and control group (without orthodontic treatment). The GCF was taken at five sites in the maxilla anterior teeth from each participant just before bracket bonding and at 1, 24, and 168 h after the initiation of tooth movement. Cytokine levels were determined through ELISA. RESULTS: The concentration of TNF-α was significantly higher in the experimental groups than in the control group at 24 h after force application. TNF-α levels were significantly decreased at 168 h after force application in the PEA group. Meanwhile, in the SL group, the level of TNF-α at 168 h was still increased, although there was no statistically significant difference. CONCLUSION: TNF-α concentration was increased at 1 h and 24 h after orthodontic force application in both the PEA and SL groups. In the PEA group, TNF-α concentration was significantly decreased at 168 h, meanwhile in the SL group, this value remained increased at this time point. The differences in TNF-α concentration between the PEA and SL groups may be caused by their different types of brackets, wires, and ligation methods.