RESUMO
Identification of mycobacterial clinical isolates by pyrosequencing within the hypervariable A region of the 16S rRNA gene was compared to other identification methods. For >90% of isolates, these identifications correlated to the level of complex or species. For identification of many mycobacteria, pyrosequencing offers an inexpensive alternative to traditional sequencing.
Assuntos
Técnicas de Tipagem Bacteriana/métodos , Infecções por Mycobacterium/microbiologia , Mycobacterium/classificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA/métodos , Cromatografia Líquida de Alta Pressão , DNA Bacteriano/análise , DNA Ribossômico/análise , Humanos , Mycobacterium/genética , Mycobacterium/isolamento & purificação , Especificidade da EspécieRESUMO
Staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene carriage were compared among suspected community-associated methicillin-resistant Staphylococcus aureus MRSA (CA-MRSA) and health care-associated MRSA (HA-MRSA) isolates. CA-MRSA isolates carried the SCCmec type IV complex, and most were PVL positive. The HA-MRSA isolates carried the SCCmec type II complex and did not harbor the PVL genes.
Assuntos
Toxinas Bacterianas/genética , Cromossomos Bacterianos/genética , Exotoxinas/genética , Leucocidinas/genética , Resistência a Meticilina/genética , Staphylococcus aureus/classificação , Antibacterianos/farmacologia , Infecções Comunitárias Adquiridas/microbiologia , Infecção Hospitalar/microbiologia , Florida , Humanos , Meticilina/farmacologia , Testes de Sensibilidade Microbiana , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/genéticaRESUMO
This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HIV-1 proviral DNA detection.
