Milk Fermented with Pediococcus acidilactici Strain BE Improves High Blood Glucose Levels and Pancreatic Beta-Cell Function in Diabetic Rats.

This study evaluated the effects of milk fermented with Pediococcus acidilactici strain BE and Pediococcus pentosaceus strain M103 on diabetes in rats (Rattus norvegicus). The bacteria were separately used as starter cultures for milk fermentation, and the products were then fed to diabetic rats for 15 days. Blood glucose levels, immunohistochemical and histological indicators, lipid profiles, and total lactic acid bacterium counts were evaluated before and after treatment. The administration of milk fermented with P. acidilactici strain BE reduced blood glucose levels from 410.27±51.60 to 304.07±9.88 mg/dL (p<0.05), similar to the effects of metformin (from 382.30±13.39 mg/dL to 253.33±40.66 mg/dL, p<0.05). Increased insulin production was observed in diabetic rats fed milk fermented with P. acidilactici strain BE concomitant with an increased number and percentage area of immunoreactive beta-cells. The structure of insulin-producing beta-cells was improved in diabetic rats fed milk fermented with P. acidilactici strain BE or metformin (insulin receptor substrate scores of 5.33±0.94 and 3.5±0.5, respectively). This suggests that the administration of milk fermented with P. acidilactici BE potentially reduces blood glucose levels and improves pancreatic beta-cell function in diabetic rats.

Exopolysaccharide production in fermented milk using Lactobacillus casei strains AP and AG.

This study evaluated the ability of two strains of bacterial starter cultures, Lactobacillus casei AP (AP) and Lactobacillus casei AG (AG), to produce exopolysaccharides (EPSs). First, the physicochemical properties of the fermented milk produced by AP and AG were assessed, including physical qualities like viscosity and syneresis and chemical qualities, such as pH, acidity, protein, lactose, fat content, and total solid. Then, AP and AG's ability to produce EPS was measured. Additionally, the EPS' microstructure was observed using a scanning electron microscope, and its chemical structure was assessed using Fourier transform-infrared (FT-IR) spectroscopy. Also, AP and AG's ability to produce EPS was tracked at the molecular level by studying the glycosyltransferase (gtf) gene. Statistical analysis showed that the milk fermented using AP and AG had similar physicochemical qualities (P > 0.05) but significantly different physical qualities (P < 0.05). Additionally, the milk fermented with AP had lower viscosity (1137.33 ± 34.31 centiPoise) than AG (1221.50 ± 20.66 centiPoise). In addition, the milk fermented using AP had higher syneresis (19.42%) than AG (17.83%). The higher viscosity and lower syneresis in the milk fermented using AG were associated with AG's ability to produce more EPS (1409 mg/L) than AP (1204 mg/L). In addition, according to the FT-IR analysis, the AP- and AG-synthesized EPS contained absorption bands at 3323, 2980, 2901, 1642, 1084, 1043, and 873 cm-1. The absorption band at 1642 and 2980 cm-1 corresponds to carbonyl and methylene groups, respectively. Absorption band 873 cm-1 is characteristic of the α-glycosidic bond of α-glucan in EPS. Moreover, the absorption bands on the wavelength region corresponding to the functional groups in the AP- and AG-produced EPS were similar to those in commercially available EPS. Lastly, gtf, contributing to EPS synthesis, was found in the genomes of AP and AG, suggesting the role of glycosyltransferase in the EPS synthesis by both strains.

Waste treatment innovation for infusion bottles using soil solution.

The amount of medical waste, especially infusion bottles, is a problem for environmental pollution. Improper management of infusion bottle waste can have an impact on disease transmission. The medical waste treatment used high technology and high costs will be a financial burden, so simple and effective treatment innovations is needed. This study uses an experimental method of removing bacteria from infusion bottles using a mixture of water and Andoso soil as a solution for washing infusion bottle waste. The soil solution concentration used in washing was 45% with a contact time of 2 minutes. The experiment was carried out with two repetitions. The treatment effect on decreasing the number of bacteria using a multiple linear regression mathematical model. The results showed that the disinfection process of bacterial-contaminated infusion bottles using water required rinsing up to six times, whereas using 45% andosol soil solution only rinsed once. The effectiveness of the disinfection of infusion bottles contaminated with bacteria using soil solution reduces the number of bacteria by 98%.

Adversity Quotient Promotes Teachers' Professional Competence More Strongly Than Emotional Intelligence: Evidence from Indonesia.

Teachers' professional competence has become a popular issue since the COVID-19 pandemic. Therefore, this study investigates teachers' professional competence, in terms of emotional intelligence (EI), adversity quotient (AQ), and organizational citizenship behavior (OCB), when teachers need to deal with abnormal situations, such as the COVID-19 pandemic. Moreover, this study also seeks to explore the relationship of EI and AQ with teachers' professional competence mediated by OCB. The research data were collected through a questionnaire using a Likert scale from 589 participants: elementary school teachers in Indonesia were chosen through accidental sampling. The data analysis used structural equation modeling (SEM), complemented by common method bias, correlational, and descriptive analysis. The result shows that EI, AQ, and OCB have a significant relationship with teachers' professional competence. However, AQ more strongly promotes teachers' OCB and professional competence than EI does. In addition, OCB mediates the relationship between EI and AQ with teachers' professional competence. Accordingly, a new model regarding the relationship of EI and AQ with teachers' professional competence mediated by OCB was confirmed. Therefore, it is suggested that teachers' professional competence can increase through EI, AQ, and OCB. Hence, the new empirical model deserves to be discussed, adapted, and even adopted by practitioners and researchers to develop the professional competence of teachers in the future.

Major Ocular Abnormalities Among Hemodialysis Patients in Indonesia.

BACKGROUND: This study aimed to identify the major ocular abnormality findings (i.e., cataract and conjunctival and corneal calcification (CCC)) among hemodialysis (HD) patients and their risk factors. METHODS: A single institute-based cross-sectional study in Indonesia. Demographic data, medical histories, and complete ocular examinations were collected. For two major ocular abnormalities found, a generalized estimating equation was incorporated in a logistic regression model to assess the relationship with their risk factors. RESULTS: We analyzed 318 eyes (159 individuals), of which 54.7% male and 45.3% female. The mean age was 51.6±11.3 years. The mean HD period was 3.5±3.2 years. Hypertension and diabetes mellitus (DM) was found in 81.1% and 34.6%, respectively. The major ocular abnormalities found were cataract (206 eyes; 64.78% (95% CI 59.53-70.03)), followed by CCC (135 eyes; 42.45% (95 % CI 37.02-47.88)). In a multivariate model, higher education (odds ratio (OR) 0.17; 95% CI 0.04-0.74), hypertension (OR 0.15; 95% CI 0.03-0.79), DM (OR 10.49; 95% CI 1.57-70.06), Systolic Blood Pressure (SBP) 120-129 mmHg (OR 0.05; 95% CI 0.003-0.69), SBP >140 mmHg (OR 0.05; 95% CI 0.004-0.67), Diastolic Blood Pressure (DBP) 80-89 mmHg (OR 7.44; 95% CI 1.13-48.73), and DBP >90 mmHg (OR 48.47; 95% CI 3.4-692.03) showed significant association with cataract. Meanwhile, there was no significant association between CCC and any predictor. CONCLUSION: Cataract and CCC were found to be the major ocular abnormalities among HD patients in this study, with DM and higher DBP as the risk factors for cataract. This finding supports recommendations for integrated regular eye screening in HD patients.

How Is Cultural Intelligence Related to Human Behavior?

Cultural intelligence is an individual's ability to recognize, understand, and adapt to cross-cultural contexts in order to change his or her self-capacity. Hence, this study explores the relationship between cultural intelligence and interpersonal communication, psychological capital (PsyCap), and organizational citizenship behavior (OCB) among teachers in Indonesia and investigates the possibility of finding relevant new models. A Likert questionnaire was used to collect research data. The research participants included 450 Indonesian junior high school teachers selected by accidental sampling. Structural equation modeling (SEM) was used for data analysis, supported by descriptive statistics and correlational matrices. The results indicate that cultural intelligence is significantly related to teachers' interpersonal communication, PsyCap, and OCB. Additionally, this study also produces a new model regarding the relationship between cultural intelligence and a teacher's OCB, mediated by interpersonal communication and PsyCap. Therefore, researchers and practitioners can discuss and adopt a new empirical model to increase cultural intelligence.

Pterygota alata (Roxb.) R.Br. Bark Fraction Induced Intrinsic Apoptotic Pathway in 4T1 Cells by Decreasing Bcl-2 and Inducing Bax Expression.

BACKGROUND AND OBJECTIVE: Pterygota alata (Roxb.) R.Br. bark extract has been studied to have cytotoxic activity on 4T1 cells. This study was conducted to determine the cytotoxic activity of several fractions of Pterygota alata (Roxb.) R.Br. bark against 4T1 breast cancer cells and to investigate the most active fractions on Bcl-2 and Bax expressions. MATERIALS AND METHODS: The bark of Pterygota alata (Roxb.) R.Br. was extracted using 80% methanol and was fractionated into fractions of n-hexane, chloroform, ethyl acetate, n-butanol and insoluble n-butanol with liquid-liquid partition. Cytotoxic tests were performed using the MTT method and expressions of Bax and Bcl-2 on 4T1 breast cancer cells were detected with immunocytochemical staining. Identification of compounds in the most active fraction using GC-MS. RESULTS: The results showed that the most active fraction was the insoluble fraction of n-butanol (IFB) with an IC50 of 15.14 µg mL-1. IFB also decreases the expression of Bcl-2 and increases the expression of Bax. CONCLUSION: It can be concluded that Pterygota alata (Roxb.) R.Br. bark has the potential to be developed for medical use, especially for breast cancer therapy.

Genomic Comparison of Lactobacillus casei AP and Lactobacillus plantarum DR131 with Emphasis on the Butyric Acid Biosynthetic Pathways.

Butyric acid is known to possess anticarcinogenic and antioxidative properties. The local lactic acid bacteria (LAB) strains Lactobacillus casei AP isolated from the digestive tract of healthy Indonesian infants and L. plantarum DR131 from indigenous fermented buffalo milk (dadih) can produce butyric acid in vitro. However, the genes and metabolic pathways involved in this process remain unknown. We sequenced and assembled the 2.95-Mb L. casei AP and 4.44-Mb L. plantarum DR131 draft genome sequences. We observed that 98% of the 2870 protein-coding genes of L. casei AP and 97% of the 3069 protein-coding genes of L. plantarum DR131 were similar to those of an L. casei strain isolated from infant stools and an L. plantarum strain in sheep milk, respectively. Comparison of the genome sequences of L. casei AP and L. plantarum DR131 led to the identification of genes encoding butyrate kinase (buk) and phosphotransbutyrylase (ptb), enzymes involved in butyric acid synthesis in L. casei AP. In contrast, a medium-chain thio-esterase and type 2 fatty acid synthase facilitated butyric acid synthesis in L. plantarum DR131. Our results provide new insights into the physiological behavior of the two LAB strains to facilitate their use as probiotics.

Analysis of pre hospital emergency management in case of head injury

INTRODUCTION: Cases of trauma still becomes a health problem in almost all countries causing death in few cases. In some developing countries, the insidences of head injury tend to increase. One of the reasons for the many death and disability may be due to the inavailability and inadequacy of pre-hospital first aid. AIM: The general objective of this study is to find out the description of community first responder in providing pre-hospital first aid to head injuries. METHODS: This study uses qualitative descriptive method. RESULTS: Most of the respondents have variety of educational backgrounds and do not have sufficient knowledge and skills to provide first aid. The average respondents provided help by performing initial assessment, managing effective airway and controlling bleeding. Limited pre-hospital facilities become one of the reasons for respondent not getting help so the efforts provided are not maximal. Respondents prefer to send patients directly to health facilities. CONCLUSION: Regular education and training programs for the community first responders should be initiated so that the number of death and disability can be minimized

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10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies.

Background We investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells. Methods For the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies. Results The in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0 µM (1.39 ± 0.24i), 12 µM (16.42 ± 0.35ab), 16 µM (17.28 ± 0.55ab), 20 µM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0 µM (17.82 ± 1.39 l), 12 µM (72.99 ± 0.31c), 16 µM (79.77 ± 4.19b), 20 µM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10-gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. Conclusions 10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT-1.

10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies.

OBJECTIVES: Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely, cumulus cells. METHODS: This study used an in vitro analysis by culturing Cumulus cells in M199 containing 10-gingerol in various concentrations (12, 16, and 20 µM) and later detected early apoptotic activity using an Annexin V-FITC detection kit. RESULT: The in vitro data revealed that the number of apoptosis cells increased along with the period of incubation as follows: 12 µM (63.71% ± 2.192%); 16 µM (74.51% ± 4.596%); and 20 µM (78.795% ± 1.435%). The substance 10-gingerol induces apoptosis in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. CONCLUSIONS: These findings indicate that further examination is warranted for 10-gingerol as a contraception agent.

Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis

Objective To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools. Methods The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis), western blot, and liquid chromatography–mass spectrometry. Results The proteins that have molecular weight (MW) 43 and 34 kDa were the antigenic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins (MW: 43 kDa) and two novel hypothetical proteins (MW: 34 kDa). Further analysis (BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that two novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi. Conclusions The identified proteins are potential to be developed as a biomarker of mosquito bite's exposure.