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1.
Nature ; 511(7509): 319-25, 2014 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-25030168

RESUMO

Malignancy is associated with altered expression of glycans and glycoproteins that contribute to the cellular glycocalyx. We constructed a glycoprotein expression signature, which revealed that metastatic tumours upregulate expression of bulky glycoproteins. A computational model predicted that these glycoproteins would influence transmembrane receptor spatial organization and function. We tested this prediction by investigating whether bulky glycoproteins in the glycocalyx promote a tumour phenotype in human cells by increasing integrin adhesion and signalling. Our data revealed that a bulky glycocalyx facilitates integrin clustering by funnelling active integrins into adhesions and altering integrin state by applying tension to matrix-bound integrins, independent of actomyosin contractility. Expression of large tumour-associated glycoproteins in non-transformed mammary cells promoted focal adhesion assembly and facilitated integrin-dependent growth factor signalling to support cell growth and survival. Clinical studies revealed that large glycoproteins are abundantly expressed on circulating tumour cells from patients with advanced disease. Thus, a bulky glycocalyx is a feature of tumour cells that could foster metastasis by mechanically enhancing cell-surface receptor function.


Assuntos
Glicocálix/metabolismo , Glicoproteínas/metabolismo , Integrinas/metabolismo , Neoplasias/metabolismo , Neoplasias/patologia , Animais , Mama/citologia , Mama/metabolismo , Mama/patologia , Linhagem Celular Tumoral , Proliferação de Células , Sobrevivência Celular , Fibroblastos , Glicocálix/química , Humanos , Proteínas Imobilizadas/química , Proteínas Imobilizadas/metabolismo , Integrinas/química , Camundongos , Terapia de Alvo Molecular , Mucina-1/metabolismo , Metástase Neoplásica/patologia , Células Neoplásicas Circulantes , Ligação Proteica , Receptores de Superfície Celular
2.
Nat Med ; 20(4): 360-7, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24633304

RESUMO

Tissue mechanics regulate development and homeostasis and are consistently modified in tumor progression. Nevertheless, the fundamental molecular mechanisms through which altered mechanics regulate tissue behavior and the clinical relevance of these changes remain unclear. We demonstrate that increased matrix stiffness modulates microRNA expression to drive tumor progression through integrin activation of ß-catenin and MYC. Specifically, in human and mouse tissue, increased matrix stiffness induced miR-18a to reduce levels of the tumor suppressor phosphatase and tensin homolog (PTEN), both directly and indirectly by decreasing levels of homeobox A9 (HOXA9). Clinically, extracellular matrix stiffness correlated directly and significantly with miR-18a expression in human breast tumor biopsies. miR-18a expression was highest in basal-like breast cancers in which PTEN and HOXA9 levels were lowest, and high miR-18a expression predicted poor prognosis in patients with luminal breast cancers. Our findings identify a mechanically regulated microRNA circuit that can promote malignancy and suggest potential prognostic roles for HOXA9 and miR-18a levels in stratifying patients with luminal breast cancers.


Assuntos
Elasticidade , Matriz Extracelular/metabolismo , Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , PTEN Fosfo-Hidrolase/metabolismo , Microambiente Tumoral , Animais , Neoplasias da Mama , Linhagem Celular , Progressão da Doença , Matriz Extracelular/genética , Feminino , Proteínas de Homeodomínio/metabolismo , Humanos , Glândulas Mamárias Animais/metabolismo , Glândulas Mamárias Humanas/metabolismo , Camundongos , MicroRNAs/fisiologia , Metástase Neoplásica/genética , Proteína Oncogênica p55(v-myc)/metabolismo , beta Catenina/metabolismo
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