RESUMO
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.
Assuntos
Clonagem Molecular , Fatores Estimuladores de Colônias/genética , DNA , Granulócitos , Macrófagos , Sequência de Aminoácidos , Animais , Sequência de Bases , Linhagem Celular , Fatores Estimuladores de Colônias/biossíntese , Fatores Estimuladores de Colônias/isolamento & purificação , DNA Recombinante , Haplorrinos , Humanos , RNA Mensageiro/genética , Linfócitos T , TransfecçãoRESUMO
We have used a mammalian cell expression cloning system to identify cDNA clones encoding human granulocyte-macrophage colony stimulating factor. The human clone was used as a hybridization probe to identify the corresponding sequence from a cDNA library prepared from a gibbon T-cell line. The human cDNA has been used to produce recombinant GM-CSF in monkey COS-1 cells. The purified protein from COS cells is very similar to the GM-CSF isolated from a continuous human T-cell line.
