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1.
PLoS One ; 16(2): e0240705, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33635869

RESUMO

In the present study, we have quantified the effects of transport, relocation and acclimate/adapt to their new surroundings on female squirrel monkey. These responses are measured in blood samples obtained from squirrel monkeys, at different time points relative to their relocation from their old home to their new home. A group of squirrel monkeys we transported, by truck, for approximately 10 hours. Peripheral blood mononuclear cells (PBMCs) were assayed in order to evaluate the phenotype of lymphocyte subsets by flow, mitogen-specific immune responses of PBMCs in vitro, and levels of cytokines at various time points including immediately before transport, immediately upon arrival, and after approximately 150 days of acclimation. We observed significant changes in T cells and subsets, NK and B cells (CD4+, CD8+, CD4+/CD8+, CD16+, and CD20+). Mitogen specific (e.g. PHA, PWM and LPS) proliferation responses, IFN-γ by ELISPOT assay, and cytokines (IL-2, IL-4 and VEGF) significant changes were observed. Changes seen in the serum chemistry measurements mostly complement those seen in the hematology data. The specific goal was to empirically assess the effects of relocation stress in squirrel monkeys in terms of changes in the numbers and functions of various leukocyte subsets in the blood and the amount of time required for acclimating to their new environment. Such data will help to determine when newly arrived animals become available for use in research studies.


Assuntos
Aclimatação/imunologia , Saimiri/imunologia , Estresse Fisiológico/imunologia , Criação de Animais Domésticos/métodos , Animais , Antígenos CD20 , Linfócitos B , Citocinas/sangue , Feminino , Leucócitos Mononucleares/classificação , Contagem de Linfócitos/métodos , Subpopulações de Linfócitos/classificação , Mitógenos , Fenótipo , Saimiri/fisiologia , Soro/química , Estresse Fisiológico/fisiologia , Linfócitos T , Meios de Transporte/métodos
2.
Virology ; 512: 180-186, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-28972927

RESUMO

Studying HIV-1 replication in the presence of functionally related proteins from different species has helped define host determinants of HIV-1 infection. Humans and owl monkeys, but not macaques, encode a CD4 receptor that permits entry of transmissible HIV-1 variants due to a single residue difference. However, little is known about whether divergent CCR5 receptor proteins act as determinants of host-range. Here we show that both owl monkey (Aotus vociferans) CD4 and CCR5 receptors are functional for the entry of transmitted HIV-1 when paired with human versions of the other receptor. By contrast, the owl monkey CD4/CCR5 pair is generally a suboptimal receptor combination, although there is virus-specific variation in infection with owl monkey receptors. Introduction of the human residues 15Y and 16T within a sulfation motif into owl monkey CCR5 resulted in a gain of function. These findings suggest there is cross-talk between CD4 and CCR5 involving the sulfation motif.


Assuntos
Antígenos CD4/fisiologia , HIV-1/fisiologia , Receptores CCR5/metabolismo , Internalização do Vírus , Sequência de Aminoácidos , Animais , Aotidae , Células HEK293 , Humanos , Mutação , Receptores CCR5/genética , Replicação Viral
3.
Comp Med ; 62(3): 229-33, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22776057

RESUMO

A 10-y-old multiparous rhesus macaque presented for an annual routine physical examination. Clinically, the animal had pale mucous membranes, petechial and ecchymotic hemorrhages in multiple sites, and a laceration at the tail base. Severe pancytopenia was noted on hematologic evaluation. The monkey was seronegative for SIV, simian T-lymphotropic virus, simian retrovirus type D, and Macacine herpesvirus 1. Bone marrow evaluation revealed a paucity of megakaryocytic precursors in a hypercellular marrow with marked erythroid hyperplasia. In light of these findings, the diagnosis was acquired amegakaryocytic thrombocytopenia purpura. Due to the poor prognosis of the syndrome and clinical deterioration of the monkey, euthanasia was elected. A definitive cause of the thrombocytopenia was not identified; however, the syndrome may have developed secondary to a recent spontaneous abortion. To our knowledge, this case represents the first reported observation of acquired amegakaryocytic thrombocytopenia purpura in a rhesus monkey.


Assuntos
Doenças da Medula Óssea/diagnóstico , Púrpura Trombocitopênica/diagnóstico , Animais , Macaca mulatta
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