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Int Endod J ; 40(4): 300-7, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17298411

RESUMO

AIM: To investigate the effects of human beta-defensins on the expression of genes involved in the host immune response of the dental pulp. METHODOLOGY: Human odontoblast-like cells were cultured in Dulbecco's modified Eagle's medium. Cells were stimulated by recombinant human beta-defensins (rhBDs) up to 4 h. RNA was extracted followed by cDNA synthesis (oligo-(dT)-primer). Samples were analysed by real-time polymerase chain reaction (PCR) technology. Genes of interest were: human beta-defensin-1, -2, interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, cyclooxygenase-2, leukotriene-A4-hydrolase, cytosolic phospholipase-A-2 (cPLA(2)), and dentine sialophosphoprotein. Gene expression of beta-actin served as internal standard for normalizing real-time PCR data. Two-way anova and the paired t-test were applied for comparison of the gene expression. RESULTS: In odontoblast-like cells rhBD-2 stimulation led to a down-regulation of the gene expression of hBD-1 (P < 0.05), whilst the mRNA expression of IL-6 (P < 0.05), IL-8 (P < 0.05) and cPLA(2) was increased in response to rhBD-2. CONCLUSION: The results of the present study suggest immune regulatory functions of human beta-defensin-2 in odontoblast-like cells.


Assuntos
Polpa Dentária/imunologia , Imunidade Inata/genética , Odontoblastos/imunologia , beta-Defensinas/imunologia , Adulto , Análise de Variância , Células Cultivadas , DNA Complementar/análise , Polpa Dentária/citologia , Regulação da Expressão Gênica , Fosfolipases A2 do Grupo IV , Humanos , Interleucina-6/biossíntese , Interleucina-6/genética , Interleucina-6/imunologia , Interleucina-8/biossíntese , Interleucina-8/genética , Interleucina-8/imunologia , Fosfolipases A/biossíntese , Fosfolipases A/genética , Fosfolipases A/imunologia , Reação em Cadeia da Polimerase , Proteínas Recombinantes/farmacologia , beta-Defensinas/biossíntese , beta-Defensinas/genética
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