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1.
Ann Work Expo Health ; 66(6): 687-697, 2022 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-35352089

RESUMO

In April 2021, the 5th German REACH Congress took place as a hybrid event with a focus on discussing interlinkages between REACH and the occupational safety and health (OSH) legislation. More than 1000 people from Germany, the EU, and non-EU countries representing all stakeholder groups participated via livestream. Around 10% of the participants provided their opinions on various issues in an online survey. The presentations and discussions, as well as the results of the online survey, show that the stakeholders perceive a positive effect of REACH on the implementation of OSH, in particular via the information made available through the registration and communication in the supply chain, and the restrictions and authorization procedures. The integration of OSH and REACH expertise in the regulatory processes, as well as transparency and clarity about the selection of regulatory instruments to manage workplace risks, were highlighted as areas for potential improvement. Overall, it was concluded that the Chemicals Strategy for Sustainability would further improve the interplay of legislation, which would also benefit the level of safety and health at workplaces.


Assuntos
Exposição Ocupacional , Saúde Ocupacional , União Europeia , Humanos , Medição de Risco/métodos , Local de Trabalho
2.
Protist ; 168(4): 375-391, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28654859

RESUMO

Heterotrophic flagellates contribute significantly to the matter flux in aquatic and terrestrial ecosystems. Still today their quantification and taxonomic classification bear several problems in field studies, though these methodological problems seem to be increasingly ignored in current ecological studies. Here we describe and test different methods, the live-counting technique, different fixation techniques, cultivation methods like the liquid aliquot method (LAM), and a molecular single cell survey called aliquot PCR (aPCR). All these methods have been tested either using aquatic field samples or cultures of freshwater and marine taxa. Each of the described methods has its advantages and disadvantages, which have to be considered in every single case. With the live-counting technique a detection of living cells up to morphospecies level is possible. Fixation of cells and staining methods are advantageous due to the possible long-term storage and observation of samples. Cultivation methods (LAM) offer the possibility of subsequent molecular analyses, and aPCR tools might complete the deficiency of LAM in terms of the missing detection of non-cultivable flagellates. In summary, we propose a combination of several investigation techniques reducing the gap between the different methodological problems.


Assuntos
Biodiversidade , Eucariotos/classificação , Eucariotos/isolamento & purificação , Hidrobiologia/métodos , Água Doce , Plâncton/classificação , Plâncton/isolamento & purificação , Água do Mar
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