RESUMO
We report on a highly ordered array of carbon nanotubes (CNTs) that serves as a universally direct nanoelectrode interface for redox proteins and provides an efficient conduit for electron transfer. The site-selective, covalent docking of the enzyme glucose oxidase (GO(x)) on the CNT tips is found to have a marked effect on enhancing electron transfer properties, as measured by cyclic voltammetry. A unimolecular electron transfer rate of 1500 s(-1) has been measured for this system, a value exceeding the rate of oxygen reduction by glucose oxidase. Furthermore, the redox enzyme-CNT array conjugate can be utilized as a quantitative, substrate-specific biosensor.
Assuntos
Técnicas Biossensoriais/instrumentação , Glucose Oxidase/química , Glucose/análise , Glucose/química , Microeletrodos , Nanotubos de Carbono/química , Técnicas Biossensoriais/métodos , Enzimas Imobilizadas/química , Desenho de Equipamento , Análise de Falha de Equipamento , Nanotubos de Carbono/ultraestrutura , Oxirredução , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Transdução de SinaisRESUMO
A previously well 54-year-old woman presented for screening mammography with a 3-day history of an inflamed lump in her right breast. She was subsequently admitted to hospital with acute melioidosis where right breast abscesses were drained. Following recovery and discharge, she was reviewed at the mammographic screening unit where her previous abnormal mammogram was found to be due to melioidosis. To our knowledge this is the first reported case of melioidosis of the breast and its appearance on mammography and ultrasound are described.
