The Impact of Environmental Enrichment on the Cortisol Level of Shelter Cats.

Enriching cats' living environment in shelters is crucial in reducing their stress. Easier access to resources allows cats to display natural behavior. This study aimed to assess whether cats staying in an enriched environment would be less stressed than cats staying in a standard environment. The first group consisted of cats living in an environment with fewer resources (standard environment)-103 cats. The second group consisted of cats living in an enriched environment-76 cats. The research material consisted of hair collected to determine the cortisol level. The results indicate that cats from a more enriched environment have almost half the level of cortisol in hair than cats from an environment with fewer resources (0.059 ng/mg vs. 0.101 ng/mg; p = 0.000001).

The influence of selected factors on wool cortisol concentration in alpacas (Vicugna pacos).

Several internal and external factors can influence animals' hormonal activity. Cortisol level in hair and wool determines chronic stress, which is connected with the long-term HPA axis effect. Wool cortisol levels in alpacas have never been determined to this time. The study aimed to assess the influence of selected factors on wool cortisol concentration in alpacas. The study included 36 alpacas. Wool samples were collected during shearing in June 2021, cut with an electric clipper from the right shoulder and the rump. Wool samples were fragmented into proximal (winter-spring regrowth) and distal (summer-fall regrowth) segments. Alpacas' Heat Stress Index (HSI) for the summer of 2020 was 139.4, and 116 for the winter of 2021. The cortisol levels in the wool samples were determined with the General Cortisol ELISA Kit assay. The most significant differences in wool cortisol concentrations were caused by two factors: the wool segment (P < 0.001; η2 = 0.889) and the region on the body (P < 0.001; η2 = 0.876). Wool cortisol level was higher in the distal segment (referring to the summer-fall season) than in the proximal one (referring to the winter-spring season). It is suggested that alpacas can feel heat stress in summer (HSI = 139.4), which could influence higher cortisol levels in the distal segment. The wool cortisol level was higher in the rump samples than the shoulder ones. Therefore, it is essential in future studies that wool samples from all tested animals should be completed from the same body region. Differences among age and sex groups were also observed. Wool cortisol level was higher in older animals, as differences between age groups were observed in samples from the rump in the distal and proximal segments (distal, the rump younger*older: P < 0.001; η2 = 0.321; proximal, the rump older*younger: P = 0.007; η2 = 0.195). Males showed higher cortisol levels than females, as a difference between sexes was observed in samples from the rump in the proximal segment (P = 0.001, η2 = 0.271). This study emphasizes that various factors may significantly influence wool cortisol levels, which can be helpful in alpacas' welfare estimation using this hormonal indicator as a noninvasive long-term stress assessment method.

Hair cortisol levels in cats with and without behavioural problems.

OBJECTIVES: The aim of the study was to analyse hair cortisol levels in cats whose owners had reported behavioural problems and undesirable behaviour. In addition, feline hair cortisol levels were compared between sexes, for indoor and outdoor cats and also for cats living in single or multi-cat households. METHODS: In total, 55 cats participated in the study, including 31 females and 24 males from various households. The cats belonged to 25 different owners who answered a questionnaire survey. The biological material used for the research were 2-3 cm fragments of hair removed at the level of the skin with scissors by the owners from the cats' lumbosacral area. The cortisol concentration in the samples was determined with the DRG Salivary Cortisol HS ELISA. Statistical analysis of the results was performed with Statistica 13.3. RESULTS: A tendency toward lower cortisol levels was found in outdoor cats vs indoor cats (U = 251.5, P = 0.066). It was also found that the cats exhibiting behavioural problems (eg, house soiling [eliminating outside the litter box]) or aggressive behaviour towards household members had statistically significantly higher hair cortisol levels (U = 162.0 [P = 0.027] and U = 9.0 [P = 0.040], respectively). CONCLUSIONS AND RELEVANCE: High levels of stress in cats can affect their interactions with other cats and with humans. The incidence of undesirable behaviour was observed more frequently in indoor cats. Significantly higher cortisol levels were found in cats that eliminated outside the litter box or that showed aggression towards their owners.

Are Hair Cortisol Levels of Humans, Cats, and Dogs from the Same Household Correlated?

Human-animal interactions and the emotional relationship of the owner with the pet are the subjects of many scientific studies and the constant interest of not only scientists but also pet owners. The aim of this study was to determine and compare the hair cortisol levels of dogs, cats, and their owners living in the same household. The owners were asked to complete a questionnaire concerning the frequency of their interactions with pets and emotional relationship with each of their cats and each of their dogs. The study involved 25 women who owned at least one dog and at least one cat. In total, 45 dogs and 55 cats from 25 households participated in the study. The average level of hair cortisol of the owners was 4.62 ng/mL, of the dogs 0.26 ng/mL, and in the hair of cats 0.45 ng/mL. There was no significant correlation between the hair cortisol level of the owner and dog or the owner and the cat and between dogs and cats living together. A significant positive correlation was observed between the hair cortisol level in the owner and the pet, for dogs in which the owner performs grooming treatments once a week and for cats which are never kissed. Although our study did not find many significant correlations, studies using other stress markers might have yielded different results.

The Relationship Between the Motor Lateralization and the Concentration of Cortisol and Tyrosine in Dogs Exposed to Stress During Veterinary Activities.

The aim of the study was to assess the relationship between the motor lateralization in dogs and the concentration of cortisol and tyrosine in their plasma during a visit to a veterinary clinic. The research group consisted of 56 dogs. Motor lateralization was tested by of an adhesive tape test. The stress intensity was assessed basing on the levels of cortisol and ft4 in the serum blood. The statistical analysis revealed that in the group of 56 dogs there were 14 left-pawed dogs, 36 right-pawed dogs, and 6 dogs did not show particular paw preference. The average cortisol levels in particular groups were as follows: 7.94 ug/dl, 3.92 ug/dl and 3.7 ug/dl, whereas the level of tyrosine in the subjects that demonstrated left-sided lateralization (mean ± SE) (1.95 ± 0.46 ng/dl) for those with right-side lateralization (1.56 ± 0.23 ng/dl) and ambilateral dogs (1.01 ± 0.22 ng/dl). The statistical calculation of Pearson's χ2 showed a significant relationship between the sex and the lateralization (χ2 = 6.238, df = 2, p = 0.0442).

Temperament Assessment Algorithm in Dogs.

The aim of this study was to evaluate the temperament of dogs on the basis of behavioral observations, with emphasis on 24 selected traits and behaviors. From the observations, the temperament of the dogs was determined and the animals were assigned to one of two personality groups: introvert or extrovert. The study involved 46 dogs. The agglomeration method, Pearson's 1-r distance, and Ward's binding method were used. As shown by the statistical analysis, 18 dogs (39%) were assessed as introverts and 28 dogs (61%) exhibited extrovert traits. To construct a model for the assessment of canine temperament using the identified traits, logistic regression was performed with the independent variables, number of extrovert traits (ETs) and introvert traits (ITs), and a dichotomous dependent variable (1 = extrovert, 0 = introvert), reflecting the assessment of the temperament of the dog based on the observations and results of the original questionnaire.

Dogs' Stay in a Pet Hotel - Salivary Cortisol Level and Adaptation to New Conditions.

The aim of this study was to assess salivary cortisol level in dogs during admission and a three-day stay in a pet hotel. The study involved 20 dogs admitted to a pet hotel for a stay lasting for at least three consecutive days. The study group consisted of 10 small dogs and 10 medium-sized dogs, eight females and 12 males. Saliva was collected each day to assess cortisol levels. A stay in a hotel is a stressful situation for dogs, as evidenced by the increase in the salivary cortisol level. The highest salivary cortisol level was observed on the day of admission to the hotel. The median salivary cortisol level in the dogs on the consecutive days was 2.7 ng/ml on admission, 2.1 ng/ml after 24 hours, and 1.9 ng/ml after 48 hours. Depending on the size of the dog, the median salivary cortisol level was 2.8 ng/ml, 2.9 ng/ml and 2.4 ng/ml in the small dogs and and 2.2 ng/ml, 1.7 ng/ml and 1.2 ng/ml in the medium sized dogs on the consecutive days.

Salivary Cortisol Interactions in Search and Rescue Dogs and their Handlers.

Search and rescue dogs are an important link in the search for missing persons. The aim of the study was to assess exam stress in search and rescue dogs and their guides. The study included 41 rescue teams taking exams of field and rubble specialties. The level of cortisol, which is the main glucocorticosteroid modulating stress reactions in humans and dogs, was analyzed. The biological material used to assess the hormone concentration was saliva collected in a non-invasive way. In total, 164 test samples were collected: two from the dog and two from the guide before and immediately after the exam. Rescue exams were shown to significantly increase salivary cortisol in both dogs and their guides. Strong interactions between cortisol levels in human-dog teams were also found with a more pronounced effect in female dog-female handler dyads.

Impact of environmental diversity of hunting complexes in the Lublin region on ontogenetic quality indicators in roe deer (Capreolus capreolus).

Populations of game are not confined to single ecosystems but function within higher-order units, e.g. ecological landscape. The basis for the establishment of the hunting complexes was the assumption that the existing game hunting grounds, i.e. the basic units implementing game management, are too small and do not cover the natural areas inhabited by game populations. Roe deer are flexible species and easily adapt to various site conditions, so they inhabit many different habitats, from large forest complexes, through small in-field tree stands and shrubs, to treeless grounds and field monocultures. The aim of the study was to determine a possible impact of environmental conditions prevailing in the hunting complexes of the Regional Directorate of State Forests (RDLP in Lublin) on the ontogenetic quality of roe deer. The study was conducted on 518 European roe deer (Capreolus capreolus) aged from 4 to 7 years (379 bucks and 139 does) harvested within hunting seasons 2010/2011-2013/2014. The results have shown that animals originating from areas with greater forest cover and denser stands are characterised by lower values of the mean ontogenetic quality parameters (carcase weight, kidney fat index, chest girth, weight of antlers) in comparison with animals from typical agricultural areas with fragmented forest complexes. These results indicate that, even in the case of such a eurytopic species as the roe deer, the ontogenetic quality differs between individual hunting complexes. The study has proved that strategies for hunting management of the roe deer should take into account the impact of the landscape structure, which provides a rationale behind creation of hunting complexes.