RESUMO
The Cytoplasmic Male Sterility (CMS)-associated region in Petunia, the S-Pcf locus, was defined by the analysis of recombinant mitochondrial genomes of somatic hybrid plants resulting from a fusion of protoplasts from CMS and fertile lines. The presence of the S-Pcf locus was shown to correlate with the CMS trait in stable somatic hybrids and in other CMS Petunia lines. A small population of unstable, sterile somatic hybrids was also generated in this fusion, most of which underwent cytoplasmic segregation in subsequent generations. Stable revertants of such sterile somatic hybrids were shown to lose the S-Pcf locus. In this paper we present a molecular and genetic analysis of unstable progenies of an unstable, sterile somatic hybrid plant derived from the same fusion experiment. Both male-sterile and fertile progenies of this somatic hybrid plant have shown continuous segregation of fertile and male-sterile progenies. All segregants in this line contained, and transcribed, the S-Pcf locus. Genetic analysis indicated the presence of various levels of multiple nuclear fertility-restoration genes in this group of progenies. These findings consolidate the association between the S-Pcf locus and the CMS trait in Petunia. It also shows that the restoration of fertility by the multiple nuclear gene system does not affect the transcription of the S-Pcf locus and that the presence of an intact S-Pcf locus is necessary in order to maintain the potential sterility in the cytoplasm.
