Bioplastic degradation and assimilation processes by a novel bacterium isolated from the marine plastisphere.

Biosourced and biodegradable plastics offer a promising solution to reduce environmental impacts of plastics for specific applications. Here, we report a novel bacterium named Alteromonas plasticoclasticus MED1 isolated from the marine plastisphere that forms biofilms on foils of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). Experiments of degradation halo, plastic matrix weight loss, bacterial oxygen consumption and heterotrophic biosynthetic activity showed that the bacterial isolate MED1 is able to degrade PHBV and to use it as carbon and energy source. The likely entire metabolic pathway specifically expressed by this bacterium grown on PHBV matrices was shown by further genomic and transcriptomic analysis. In addition to a gene coding for a probable secreted depolymerase, a gene cluster was located that encodes characteristic enzymes involved in the complete depolymerization of PHBV, the transport of oligomers, and in the conversion of the monomers into intermediates of central carbon metabolism. The transcriptomic experiments showed the activation of the glyoxylate shunt during PHBV degradation, setting the isocitrate dehydrogenase activity as regulated branching point of the carbon flow entering the tricarboxylic acid cycle. Our study also shows the potential of exploring the natural plastisphere to discover new bacteria with promising metabolic capabilities.

A critical review on the evaluation of toxicity and ecological risk assessment of plastics in the marine environment.

The increasing production of plastics together with the insufficient waste management has led to massive pollution by plastic debris in the marine environment. Contrary to other known pollutants, plastic has the potential to induce three types of toxic effects: physical (e.g intestinal injuries), chemical (e.g leaching of toxic additives) and biological (e.g transfer of pathogenic microorganisms). This critical review questions our capability to give an effective ecological risk assessment, based on an ever-growing number of scientific articles in the last two decades acknowledging toxic effects at all levels of biological integration, from the molecular to the population level. Numerous biases in terms of concentration, size, shape, composition and microbial colonization revealed how toxicity and ecotoxicity tests are still not adapted to this peculiar pollutant. Suggestions to improve the relevance of plastic toxicity studies and standards are disclosed with a view to support future appropriate legislation.

Comparative study of response of four crop species exposed to carbon nanotube contamination in soil.

Crop plants are exposed to a variety of contaminants through sewage sludge spreading but very little is known about the impact of emerging contaminants such as nanomaterials. To date their impact on plants is still very controversial with many works claiming negative impacts while some authors suggest their use as plant growth regulator in agriculture. In this study, aiming to better understand where these discrepancies may come from, we investigated the influence of plant species (tomato, rapeseed, cucumber and maize) on plant response to a carbon nanotube contamination in soil condition. Our results demonstrate that the same CNT contamination can lead to different effects depending on plant species with positive impacts on cucumber and rapeseed (more than 50% increase in leaf biomass and surface area and 29% increase in chlorophyll for cucumber) but negative impact on maize (-14% for plant height), while tomato was insensitive. FTIR analysis of biomacromolecule composition suggested that these differences could be related with plant cell wall composition (in particular: pectins, xyloglucans and lignins). As a summary, no overall conclusion can be drawn about the toxicity of a specific nanomaterial for all plant species.