The PRR-EC complex and SWR1 chromatin remodeling complex function cooperatively to represses nighttime hypocotyl elongation by modulating PIF4 expression in Arabidopsis.

The circadian clock entrained by environmental light-dark cycles allows plants to fine-tune diurnal growth and developmental responses. Here, we show that physical interactions among evening clock components, including PSEUDO-RESPONSE REGULATOR5 (PRR5), TIMING OF CAB EXPRESSION1 (TOC1), and the Evening Complex (EC) component EARLY FLOWERING 3 (ELF3), define a diurnal repressive chromatin structure specifically at the PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) locus in Arabidopsis. These three clock components act interdependently as well as independently to repress nighttime hypocotyl elongation, as hypocotyl elongation rate dramatically increased specifically at nighttime in the prr5-1 toc1-21 elf3-1 mutant concomitant with a substantial increase in PIF4 expression. Transcriptional repression of PIF4 by ELF3, PRR5, and TOC1 is mediated by the SWI2/SNF2-RELATED (SWR1) chromatin remodeling complex, which incorporates histone H2A.Z at the PIF4 locus, facilitating robust epigenetic suppression of PIF4 during the evening. Overall, these findings demonstrate that the PRR-EC-SWR1 complex represses hypocotyl elongation during the night through a distinctive chromatin domain covering the PIF4 chromatin.

Overexpression of the WOX5 gene inhibits shoot development.

WUSCHEL-RELATED HOMEOBOX 5 (WOX5) is a member of the WUSCHEL (WUS) homeodomain transcription factor family. WOX5 is expressed mainly in the quiescent center (QC) and confers stem cell identity in the root apical meristem (RAM). Consistent with the role of WUS in repressing root meristem development, we found that ectopic expression of WOX5 disrupted shoot development by repressing shoot-related genes, such as YABBY1 (YAB1). Our findings suggest that WOX5 and WUS potentially confer different tissue identities and specify RAM and SAM, respectively.

Erratum: Brassinosteroids Regulate Circadian Oscillation via the BES1/TPL-CCA1/LHY Module in Arabidopsis thaliana.

[This corrects the article DOI: 10.1016/j.isci.2020.101528.].

Brassinosteroids Regulate Circadian Oscillation via the BES1/TPL-CCA1/LHY Module in Arabidopsisthaliana.

Brassinosteroids (BRs) regulate a variety of physiological processes in plants via extensive crosstalk with diverse biological signaling networks. Although BRs are known to reciprocally regulate circadian oscillation, the molecular mechanism underlying BR-mediated regulation of circadian clock remains unknown. Here, we demonstrate that the BR-activated transcription factor bri1-EMS-SUPPRESSOR 1 (BES1) integrates BR signaling into the circadian network in Arabidopsis. BES1 repressed expression of CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) at night by binding to their promoters, together with TOPLESS (TPL). The repression of CCA1 and LHY by BR treatment, which occurred during the night, was compromised in bes1-ko and tpl-8 mutants. Consistently, long-term treatment with BR shortened the circadian period, and BR-induced rhythmic shortening was impaired in bes1-ko and tpl-8 single mutants and in the cca1-1lhy-21 double mutant. Overall, BR signaling is conveyed to the circadian oscillator via the BES1/TPL-CCA1/LHY module, contributing to gating diurnal BR responses in plants.