RESUMO
BACKGROUND: Zehneria scabra is one of the folklore plants of Ethiopia frequently used for external wound treatment in humans. Nevertheless, pharmacological investigations have not been performed to substantiate activity of the plant extract in wound healing. Hence, this study attempted to explore the activity of leaf extract of Z. scabra in wound healing using a mice model. MATERIALS AND METHODS: The air-dried leaf from Z. scabra was pulverized and extracted with 80% methanol and prepared with 5% and 10% extract ointments. An acute dermal toxicity study of the extract was conducted in female mice by observing the signs of toxicity. Then 5% and 10% (w/w) ointments of the extract were applied topically to investigate their wound healing activity in incision and excision wound models. Parameters such as wound contraction, period of epithelialization, and tensile strength were determined. RESULTS: Upon the application of 10% w/w extract ointment, no signs of dermal toxicity were observed in mice. Both 5% and 10% (w/w) extract ointment formulations increased percentage wound contraction and tensile strength, and shortened the epithelialization period. CONCLUSION: The findings of this study collectively showed that the leaf extract of Z. scabra possessed significant wound healing activity, upholding the folkloric use of the experimental plant.
RESUMO
BACKGROUND: Patients are frequently provided with medicine information materials (MIMs). Rendering medicine information through written material is a reliable method. Readability is an important attribute of written material that can affect the reader's ability to comprehend. Patient's perception can also affect the comprehensibility of written MIMs. OBJECTIVE: The objectives of the study were to assess the readability of medicine information in Tikur Anbessa Specialized Hospital (TASH); and assessing patients' perception and understanding of medicine information materials. METHODS: This was a cross-sectional study conducted from September 21, 2019 to November 24, 2020, at TASH. Quantitative and qualitative data collection approaches were used in this research. The readability value of each material was determined in accordance with the Flesch Reading ease scores (FRE) and Flesch-Kincaid Grade Level (FKGL). The tools compute readability based on an average number of syllables per word and an average number of words per sentence. FRE provides scores from 0 to 100; higher scores mean easily comprehensible while FKGL sets grade levels for written texts. A structured interview was administered with questions about how MIMs had been used, and was analyzed qualitatively. RESULTS: The results of this research showed low readability scores of MIMs found in TASH. Most patients do not get MIMs and are unaware of how to use them. They are interested to receive and read medicines information from pharmacists and physicians. Moreover, most of them preferred information through both verbal and written forms. CONCLUSION: The readability levels of selected MIMs obtained from TASH are found to be not compliant with the patients' needs. This might be worsening their health outcomes and resulting in poorer use of healthcare services.
RESUMO
Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages. Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively. Thus, the current study aims to isolate cellulose fibers and CNCs from KW as alternative source. The KW, cellulose fibers, and CNCs were investigated for yield, chemical composition, functionality, crystallinity, morphology, and thermal stability. CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily. The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers. The XRD results indicated that chemical pretreatments and acid hydrolysis significantly increased the crystallinity of cellulose fibers and CNCs. The cellulose fibers and CNCs exhibited Cellulose Iß crystalline lattice. TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73). The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively. CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability. Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h. This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.
