Ecological and human health hazards of soil heavy metals after wildfire: A case study of Liangshan Yi autonomous prefecture, China.

Soil samples were collected in at different depths from the conflagration area in Liangshan Yi Autonomous Region, China, to investigate the distribution characteristics and ecological and human health risks of heavy metals after a wildfire. The samples collected comprise wildfire ash (WA) above the soil surface, ash soil (AS) 0-5 cm, and plain soil (PS) 5-15 cm below the soil surface. Additionally, reference soil (RS) was collected from a nearby unburned area at the same latitude as the conflagration area. The results showed that the concentrations of zinc (Zn), copper (Cu), lead (Pb), and cadmium (Cd) in the WA and AS were significantly higher than in reference soil (RS) (p < 0.05). Concentrations of Pb in the PS were 2.52 times higher than that in RS (17.9 mg kg-1) (p < 0.05). The AS and WA had the highest Index of potential ecological risks (RI > 600). In addition, The Cd in AS and WA contributed the most to the highest Improved nemerow index (INI) and RI with a contribution of more than 80%. The concentration of heavy metals was used to establish non-carcinogenic effects and cancer risks in humans via three exposure pathways: accident ingestion of soil, dermal contact with soil, and inhalation of soil particles. Hazard index (HI) values of each sample were all less than 1, indicating the non-carcinogenic risk was within the acceptable range and would not adversely affect the local population's health. The Cancer risk (CR) values of Cr, As, Cd, and Ni were all below 1 × 10-6, indicating that heavy metal pollution from this wildfire did not pose a cancer risk to residents.

Effects of biochar on the manganese enrichment and oxidation by a microalga Scenedesmus quadricauda in the aquatic environment.

Microalgae play a significant impact in the biogeochemical cycle of Mn(II) in the aquatic ecosystem. Meanwhile, the inflow of biochar into the water bodies is bound to impact the aquatic organisms. However, the influence of biochar on the manganese transformation in algae-rich water has not drawn much attention. Thus, we studied the effects of rice straw biochar on manganese enrichment and oxidation by a common type of algae in freshwater (Scenedesmus quadricauda). The results showed that Mn(II) was absorbed intracellularly and adsorbed extracellularly by active algal cells. A significant portion of enriched Mn(II) was oxidized to amorphous precipitates MnO2, MnOOH, and Mn2O3. Moreover, the extracellular bound Mn(II) content in the coexistent system of algae and biochar increased compared with the pure Scenedesmus quadricauda system. Nevertheless, the intracellular Mn content was continually lowered as the biochar dose rose from an initial 0.2 to 2.0 g·L-1, suggesting that Mn assimilation of the cell was suppressed. It was calculated that the total enrichment ability of Scenedesmus quadricauda in the algae-biochar coexistent system was 0.31- 15.32 mg Mn/g biomass, more than that in the pure algae system. More importantly, with biochar in the algae system, the amount of generated MnOx increased, and more Mn(II) was oxidized into highly-charged Mn(IV). This was probably because the biochar could relieve the stress of massive Mn(II) on algae and support the MnOx precipitates. In brief, moderate biochar promoted the Mn(II) accumulation by algal cells and its oxidation activity. This study offers deeper insight into the bioconversion of Mn(II) by algae and the potential impact of biochar application to the aquatic system.

Metabolomics Reveal Nanoplastic-Induced Mitochondrial Damage in Human Liver and Lung Cells.

Plastic debris in the global biosphere is an increasing concern, and nanoplastic (NPs) toxicity in humans is far from being understood. Studies have indicated that NPs can affect mitochondria, but the underlying mechanisms remain unclear. The liver and lungs have important metabolic functions and are vulnerable to NP exposure. In this study, we investigated the effects of 80 nm NPs on mitochondrial functions and metabolic pathways in normal human hepatic (L02) cells and lung (BEAS-2B) cells. NP exposure did not induce mass cell death; however, transmission electron microscopy analysis showed that the NPs could enter the cells and cause mitochondrial damage, as evidenced by overproduction of mitochondrial reactive oxygen species, alterations in the mitochondrial membrane potential, and suppression of mitochondrial respiration. These alterations were observed at NP concentrations as low as 0.0125 mg/mL, which might be comparable to the environmental levels. Nontarget metabolomics confirmed that the most significantly impacted processes were mitochondrial-related. The metabolic function of L02 cells was more vulnerable to NP exposure than that of BEAS-2B cells, especially at low NP concentrations. This study identifies NP-induced mitochondrial dysfunction and metabolic toxicity pathways in target human cells, providing insight into the possibility of adverse outcomes in human health.

Spatiotemporal distribution, source apportionment and risk assessment of typical hormones and phenolic endocrine disrupting chemicals in environmental and biological samples from the mariculture areas in the Pearl River Delta, China.

The present work studied the levels, distribution, potential sources, ecological and human health risks of typical hormones and phenolic endocrine disrupting chemicals (EDCs) in the mariculture areas of the Pearl River Delta (PRD), China. The environmental levels of 11 hormones (6 estrogens, 4 progestogens, and 1 androgen) and 2 phenolic EDCs were quantified in various matrices including water, sediment, cultured fish and shellfish. Ultrahigh performance liquid chromatography-triple quadrupole tandem mass spectrometry analyses showed that all the 13 target compounds were detected in biotic samples, whereas 10 were detected in water and sediment, respectively. The total concentrations ranged from 35.06-364.53 ng/L in water and 6.31-29.30 ng/g in sediment, respectively. The average contaminant levels in shellfish (Ostrea gigas, Mytilus edulis and Mimachlamys nobilis) were significantly higher than those in fish (Culter alburnus, Ephippus orbis and Ephippus orbis). Source apportionment revealed that the pollution of hormones and phenolic EDCs in PRD mariculture areas was resulted from the combination of coastal anthropogenic discharges and mariculture activities. The hazard quotient values of the contaminants were all less than 1, implying no immediate human health risk. Overall, the present study is of great significance for scientific mariculture management, land-based pollution control, ecosystem protection, and safeguarding human health.

Bioaccumulation and health risk assessment of phthalate esters in cultured low trophic level fish feded with food waste-based diets.

The present study aimed at using different types of food waste as a protein source for the fish feeds to produce safe and quality fish. The results showed the concentrations of ∑PAEs in grass crap (0.24 µg g-1 ww) and bighead carp (0.15 µg g-1 ww) fed with Food waste B feeds (mainly containing fruit, vegetable and cereal waste) was significantly lower than those fed Food waste A feeds (mainly containing meat waste) and control feeds (p<0.05), and the highest ∑PAEs concentration (1.75 µg g-1 ww) was found in the mud carp fed with control feeds in experimental ponds (p<0.05). In addition, there was no biomagnification of PAEs in the food chains within the ponds. The health risk assessment results showed no non-carcinogenic and carcinogenic risks from the PAEs via ingestion of the fish fed with food waste feeds for the local residents.

Adsorption of microcystin contaminants by biochars derived from contrasting pyrolytic conditions: Characteristics, affecting factors, and mechanisms.

The growing incidence of microcystins (MCs) in the environment has become an issue of global concern for the high ecological and human health risks. Herein, a comparative adsorption of three MCs (MC-LR, MC-YR and MC-RR) by spent mushroom substrate (SMS)-derived biochars from contrasting pyrolytic conditions (temperature: 600/300 °C; and gas steam: CO2/N2) was surveyed to better understand the mechanisms and factors affecting the adsorption performance. For biochar preparation, 600 °C and CO2 led to greater levels of aromaticity, ash, SBET, and porosity, while 300 °C and N2 created more surface functional groups. The adsorption of MCs by biochars was a pH-dependent and endothermic physisorption process, following the pseudo-second-order kinetics (R2 = 0.99) and linear isotherm model (R2 > 0.88). The distribution coefficients Kd (0.98-19.2 L/kg) varied greatly among MCs (MC-YR > MC-RR > MC-LR) and biochars (BC600 > BN600 > BC300 > BN300), which depends on the combined effects of hydrophobicity, electrostatic attraction, H-bonding, cation bridging, and the amounts of adsorption sites on biochars. Higher ash, SBET, and total pore volume of BC600 facilitated the adsorption capacity for MCs relative to other biochars. Furthermore, the co-adsorption efficacy for MCs (Kd = 1.09-8.86 L/kg) was far below those for the single adsorption, indicating strong conflicts among competing MCs. This study sheds light on the roles of pyrolytic temperature and gas steam in biochar properties, and elucidates the mechanisms and factors affecting the adsorption performance of different MCs, which lays a foundation for MCs removal from water.

A Visual Leaf Zymography Technique for the In Situ Examination of Plant Enzyme Activity under the Stress of Environmental Pollution.

This study established a high-efficiency fluorescence quenching approach for the in situ visualization and modeling of the spatial distribution of xylanase, ß-glucosidase, and phosphatase activities in plant leaves under pollution stress (namely, the leaf zymography technique, LZT). In the LZT, a membrane saturated with an enzyme-specific fluorescent substrate on the leaf surface was incubated and the fluorescence image generated on the membrane under ultraviolet light was recorded. An image-based modeling method for restoring the morphological traits of the true image by reducing noise was developed to ensure the accurate estimation of enzyme activities. The LZT could simultaneously measure 48 samples within 2 h, with good reproducibility. The results obtained by the LZT were comparable to those obtained by a conventional biochemical analysis method and presented low-cost and convenient advantages. This paper explains the theoretical basis required to investigate the realistic application of the LZT for assessing ecotoxicity in large-scale monitoring.

Prevalent phthalates in air-soil-vegetable systems of plastic greenhouses in a subtropical city and health risk assessments.

Wide use of plastic greenhouses for vegetable production increases human exposure to phthalate (PAEs) through vegetable intake. However, little information is available about distribution of PAEs in air-soil-vegetable systems of plastic greenhouses and PAE estrogenic effects. This study was designed to investigate PAE distributions and corresponding health risk in plastic greenhouses in Guangzhou, a subtropical city in South China. PAEs were prevalent in plastic greenhouses, with sum concentrations of 16 PAE compounds (∑16PAEs) up to 5.76 mg/kg in soils, 5.27 mg/kg in vegetables and 4393 ng/m3 in air. Di (2-ethylhexyl) phthalate, di-isobutyl phthalate, and dibutyl phthalate were predominant compounds. Average concentrations and bioconcentration factor of ∑16PAEs and the predominant PAE compounds in vegetables of greenhouses were higher than those of open fields. Plastic greenhouses exhibited significantly higher air PAE levels than those of open fields due to higher indoor temperature, which enhanced PAE accumulation by vegetables. Both carcinogenic and non-carcinogenic risks of PAEs via dietary and non-dietary exposures for farmers decreased with an order of vegetable > air > soil. Consumption of vegetables from greenhouses resulted in significantly higher estrogenic effects compared to those from open field cultivation. This study emphasizes highly potential health risks of PAEs in air-soil-vegetable systems of plastic greenhouses.

Improving yield and quality of vegetable grown in PAEs-contaminated soils by using novel bioorganic fertilizer.

Phthalate acid esters (PAEs) are ubiquitous pollutants in agricultural soils. Application of bioorganic fertilizer (BOF) containing beneficial microbes represents a promising approach to improve the yield and quality of crops grown in contaminated soils. In the present study, a novel multifunctional bioorganic fertilizer N-BOF was developed by using compost of sewage sludge and agricultural waste and inoculating with PAEs-degrading B. megaterium YJB3 and phosphate solubilizing B. megaterium YLYP1. Its feasibility of improving the yield and quality of vegetable grown in PAEs (including DBP and DEHP) contaminated soil was evaluated by pot experiments. The N-BOF could effectively promote plant growth, with biomass increasing by 4-66.9% and 19-110% compared to chemical (CF) and no fertilizer (CK), respectively. The concentrations of DBP and DEHP in shoots of chemically fertilised vegetable ranged 1.23-3.12 mg/kg (dry weight, DW) and 1.63-3.89 mg/kg (DW), respectively. Their concentrations were significantly decreased (p < 0.05) when N-BOF was applied (1%, 2%, 5% amendment), especially at higher application rate ranging 0.11-0.3 mg/kg (DW) and 0.16-0.32 mg/kg (DW), respectively. Meanwhile, vegetable quality attributes were also significantly improved when 2% N-BOF was applied, with increase in the contents of vitamin C, vitamin B1, total protein, and starch, and decrease in the contents of nitrite and nitrate. In this case, the human health risk from consumption of the vegetable grown in PAEs-contaminated soil could be significantly reduced. Thus, our study is expected to provide an efficient way of high-value utilization of organic substrates by producing low-cost but high quality N-BOF. Future studies on the effects of N-BOF in terms of fertilizer regimes on yield and quality of the vegetable are needed, and further field studies for assessing the long-term efficacy and reliability of this promising N-BOF are also warranted.

Source, migration and toxicology of microplastics in soil.

Microplastics are emerging contaminants and their presence in water and soil ecosystems has recently drawn considerable attention because they pose a great threat to entire ecosystems. Recent researches have focused on the detection, occurrence, characterization, and toxicology of microplastics in marine and freshwater ecosystems; however, our understanding of the ecological effects of microplastics in soil ecosystems is still limited compared with that in aquatic ecosystems. Here, we have compiled literature, studying the sources, migration of microplastics in soil, negative impacts on soil health and function, trophic transfer in food chains, and the corresponding adverse effects on soil organisms in order to address the potential ecological and human health risks caused by microplastics in soil. This review aims to address gaps in knowledge, shed light on the ecological effects of microplastics in soil, and propose future studies on microplastic pollution and the resultant soil ecotoxicity. Furthermore, this review is focused on limiting microplastics in soil and establishing management and remediation measures to mitigate the risks posed by microplastic pollution.

Insights into the binding interaction of substrate with catechol 2,3-dioxygenase from biophysics point of view.

This study aims to clarify the interaction mechanism of substrate with catechol 2,3-dioxygenase (C23O) through multi-technique combination. A novel C23O (named C23O-2G) was cloned, heterogeneously expressed, and identified as a new member in subfamily I.2 of extradiol dioxygenases. Based on the simulations of molecular docking and dynamics, the exact binding sites of catechol on C23O-2G were identified, and the catalytic mechanism mediated by key residues was proposed. The roles of the predicted residues during catalysis were confirmed by site-directed mutagenesis, and the mutation of Thr254 could significantly increase catalytic efficiency and substrate specificity of C23O-2G. The binding and thermodynamic parameters obtained from fluorescence spectra suggested that catechol could effectively quench the intrinsic fluorescence of C23O-2G via static and dynamic quenching mechanisms and spontaneously formed C23O-2G/catechol complex by the binding forces of hydrogen bond and van der Waals force. The results of UV-vis spectra, synchronous fluorescence, and CD spectra revealed obvious changes in the microenvironment and conformation of C23O-2G, especially for the secondary structure. The atomic force microscope images further demonstrated the changes from an appearance point of view. This study could improve our mechanistic understanding of representative dioxygenases involved in aromatic compound degradation.

High ecological and human health risks from microcystins in vegetable fields in southern China.

Frequent cyanobacterial blooms in the eutrophic waters produce a variety of toxins such as the monocyclic heptapeptide microcystins, greatly harming aquatic ecosystems and human health. However, little information of microcystins in agricultural fields is known. This field study of three common microcystin variants (MC-LR, MC-RR, and MC-YR) in vegetables (n = 161), soils (n = 161) and irrigation water samples (n = 23) collected from southern China regions affected by cyanobacteria blooms, shows their prevalence with total concentrations up to 514 µg/L water, 187 µg/kg soil (dry weight) and 382 µg/kg vegetable (fresh weight). MC-RR was the primary variant in all types of samples, accounting for 51.3-100% of total microcystin concentrations. Significant concentration-dependent correlations (p < 0.05) demonstrated that microcystin-contained irrigation waters were the major source of microcystin accumulation in both vegetables and soils. Meanwhile, intracellular-microcystins in irrigation water was found to play an important role in microcystins bioaccumulation in vegetables for the first time. Most vegetable samples (≥60%), particularly celery posed moderate or high human health risk via diet based on toxicity equivalents of the microcystins and reference dose for MC-LR (0.04 µg/kg/d), showing high food safety hidden dangers. Soil microcystins, especially MC-RR in 46.4-88.3% of soils could pose high ecological risks. This study highlights the potential high ecological and human health risks of microcystins in the real soil-vegetable systems of areas affected by cyanobacteria blooms, implying the profound significance and urgent need of investigation on microcystins in terrestrial ecosystems.

Distribution, diastereomer-specific accumulation and associated health risks of hexabromocyclododecanes (HBCDs) in soil-vegetable system of the Pearl River Delta region, South China.

The distribution and diastereomeric profiles of hexabromocyclododecanes (HBCDs, identified as persistent organic pollutants) in soil-vegetable system of open fields remain unknown. In this study, three main HBCD diastereoisomers (α-, ß-, and γ-HBCDs) were analyzed in paired soil and vegetable samples from vegetable farms in four cities (Guangzhou, Jiangmen, Huizhou, Foshan) of the Pearl River Delta region, Southern China. The sum concentrations of the three diastereoisomers (∑HBCDs) in soils varied from 0.99 to 18.4 ng/g (dry weight) with a mean of 5.77 ng/g, decreasing in the order of Jiangmen > Guangzhou > Huizhou > Foshan. The distributions of HBCDs in both soil and vegetable were diastereomer-specific, with γ-HBCD being predominant. The ∑HBCDs in vegetables ranged from 0.87 to 32.7 ng/g (dry weight) with a mean of 16.6 ng/g, generally higher than those of the corresponding soils. Thus bioconcentration factors (BCFs, the ratio of contaminant concentration in vegetable to that in soil) of HBCDs were generally greater than 1.0, implying higher accumulation in vegetable. The estimated daily intake (EDI) of ΣHBCDs via consumption of vegetables varied from 0.26 to 9.35 ng/kg bw/day with a mean of 3.60 ng/kg bw/day for adults and from 0.32 to 11.5 ng/kg bw/day with a mean of 4.41 ng/kg bw/day for Children, far lower than the oral reference dose (RfD, 2 × 105 ng/kg bw/day) proposed by US National Research Council. These results suggest that HBCD in the vegetables posed low health risk for the local population. These data are the first report on HBCD occurrence and health risk in soil-vegetable system of open fields.

Bisphenol S-induced chronic inflammatory stress in liver via peroxisome proliferator-activated receptor γ using fish in vivo and in vitro models.

Bisphenol S (BPS) has been widely used as a bisphenol alternative in recent few years. However, with mounting evidence suggesting that the presence of BPS in the environment also poses risks to ecosystems and human health, we decided to use the juvenile common carp (Cyprinus carpio) and its primary macrophages as in vivo and in vitro models to examine if BPS is a safe substitute of BPA. The present study evaluated the immune responses of chronic BPS exposure and their mechanisms of action associated with peroxisome proliferator-activated receptor (PPAR) signaling pathway. Potential oxidative stress and pro-inflammatory effects of BPS exposure were identified in fish liver after 60-day exposure, based on the increased reactive oxygen species (ROS) production, antioxidant capacity, NO production, lipid peroxidation, and induction of inflammatory cytokine expression, as well as acute phase protein levels of C-reactive protein, immunoglobulin M, lysozyme, and complement component 3. Moreover, pparγ, PPAR pathway-associated genes retinoid x receptor α (rxrα) and nuclear factor-κb (nfκb) presented a rough concentration-dependent alteration after BPS exposure. An acute BPS exposure to the isolated primary macrophages from juvenile common carp was performed to help elucidate gene expression patterns of pparγ, rxrα, and nfκb in a typical immune cell model, the results were consistent with what we found in vivo experiments for long-term BPS exposure. Furthermore, with coexposure to BPS and a PPARγ antagonist, the restriction of PPAR signaling pathway significantly inhibited the induction of ROS and the mRNA level of interleukin-1ß, confirming the involvement of PPAR pathway in BPS-induced chronic inflammatory stress in liver.

Sorption Mechanism, Kinetics, and Isotherms of Di- n-butyl Phthalate to Different Soil Particle-Size Fractions.

Di- n-butyl phthalate (DBP) is a prevalent pollutant in agricultural soils due to use of plastic film. This study focused on sorption mechanism, kinetics, and isotherms of DBP to six paddy soil particle-size fractions (i.e., coarse sand, fine sand, coarse silt, fine silt, clay, and humic acid fractions). DBP sorption involved in both boundary layer diffusion and intraparticle diffusion, following pseudo-second-order kinetics. DBP sorption was a spontaneous physical process, which fit the Freundlich model. Hydrophobic and ionic interaction relevant to the organic matter content, cation exchange capacity, surface area, and pore volume of soil fractions played key roles in DBP sorption. DBP was strongly adsorbed to humic acid and the sorption was reversely associated with soil particle sizes. DBP may exhibit higher mobility and bioavailability in a soil-crop system at lower temperature (15 °C), due to the lower log Koc values.

Metabolism disruption analysis of zebrafish larvae in response to BPA and BPA analogs based on RNA-Seq technique.

Bisphenol A (BPA) is an environmentally ubiquitous chemical widely used in industry and is known to have adverse effects on organisms. Given the negative effect, BPA-free products have been developed with BPA analogs such as bisphenol F (BPF) and bisphenol S (BPS); however, these analogs are proving to exhibit toxicity similar to that of BPA. In the present study, we aimed to identify and compare the underlying mechanisms of toxicity of BPA, BPF, and BPS at the transcriptional level by conducting global transcriptome sequencing (RNA-Seq) on zebrafish embryos. RNA-seq results showed that the expression levels of 285, 191, and 246 genes were significantly changed in zebrafish larvae after embryos were treated for 120 h with 100 µg/L BPA, BPF, and BPS, respectively. Among the genes exhibiting altered expression, a substantial number were common to two or three exposure groups, suggesting consistent toxicity between the three bisphenols. We further validated the expression levels of 19 differentially expressed genes by qRT-PCR, using sequencing RNA and the RNA samples after treatment by 0.01, 1, and 100 µg/L bisphenols under identical condition, the results were similar to RNA-Seq. Moreover, functional enrichment analysis indicated that metabolism was the main pathway which disrupted in zebrafish larvae by bisphenols treatment. Protein-protein interaction network analysis indicated that six DEGs (ces, cda, dpyd, upp1, upp2, and cmpk2) interact together in the drug metabolism of zebrafish. In summary, our study revealed changes in the transcription of genes upon bisphenols treatment in zebrafish larvae for the first time, indicating that BPF and BPS may cause adverse effects similar to BPA via their involvement in various biological processes, providing a solid foundation for further research on the toxicology of BPA analogs.

Evaluation of gas chromatography-atmospheric pressure chemical ionization tandem mass spectrometry as an alternative to gas chromatography tandem mass spectrometry for the determination of polychlorinated biphenyls and polybrominated diphenyl ethers.

A method for the analysis of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in serum was developed using gas chromatography coupled to tandem mass spectrometry with atmospheric pressure chemical ionization (GC-APCI-MS/MS). The ionization and fragmentation performance of APCI were evaluated and compared with those of electron ionization (EI). In contrast to extensive fragmentation caused by EI with high energy, soft ionization achieved by APCI produced mass spectra dominated by molecular ions from the first stage of MS analysis. Better sensitivity and selectivity achieved by the GC-APCI-MS/MS method allowed to analyze serum samples with a low volume (100 µL). The limits of detection (LODs) ranged from 0.067 to 14 pg/mL for the analysis in serum samples. The developed method was evaluated at three spiking levels (0.05, 0.5 and 5 ng/mL for PCBs), showing good recoveries and repeatability. The recoveries ranged from 74.0% to 130.5%, and the relative standard deviations (RSDs) were less than 20%, for all analytes. The determination of PCBs and PBDEs in the human serum samples by GC-APCI-MS/MS was compared with gas chromatography-tandem mass spectrometry with EI (GC-EI-MS/MS). BDE-99 and BDE-100 were successfully quantified by GC-APCI-MS/MS, while these two PBDE congeners were not detected by GC-EI-MS/MS. The GC-APCI-MS/MS method had a clear advantage when analyzing compounds at low levels.

Co-metabolic degradation of the antibiotic ciprofloxacin by the enriched bacterial consortium XG and its bacterial community composition.

Ciprofloxacin is a broad spectral and highly refractory antibiotic. It is an emerging pollutant. This study aimed to utilise co-metabolism as a means to degrade ciprofloxacin by a bacterial consortium. The stable bacterial consortium XG capable of efficiently degrading ciprofloxacin was successfully established through successive acclimation of indigenous microorganisms. The consortium XG was primarily consisted of Achromobacter, Bacillus, Lactococcus, Ochrobactrum, and Enterococcus as well as at least other five minor genera. A novel strain YJ17 with CIP-degrading ability was isolated from the consortium and identified as Ochrobactrum sp. The consortium XG utilised amino acids, carbohydrates, and carboxylic acids at a rate approximately 16.6-243-fold greater than the other carbon substrates, but only slow utilisation of ciprofloxacin as a sole carbon source. Ciprofloxacin can be co-metabolized along with many carbon sources, attaining degradation rates up to 63%. Glycyl-l-glutamic acid, d-cellobiose, and itaconic acid are among the substrates most favourable for co-metabolism. The metabolites of ciprofloxacin were identified by LC-QTOF-MS. Co-metabolic degradation of ciprofloxacin by consortium XG led to the removal of essential functional groups from parent compound, thus resulting in formation of metabolites with less bioactive potency. Finally, a possible biochemical pathway for the degradation of ciprofloxacin was proposed. Consortium XG possesses high potential for bioremediation of ciprofloxacin-contaminated environments in the presence of a co-substrate.

The occurrence, potential toxicity, and toxicity mechanism of bisphenol S, a substitute of bisphenol A: A critical review of recent progress.

Bisphenol S (BPS) has been introduced into the industry as a safer alternative to bisphenol A (BPA). The distribution of BPS has recently become an important issue worldwide, but investigations on the toxicity and mechanisms of BPS remain limited. A review of the literature reveals that BPS has widespread presence in environmental media, such as indoor dust, surface water, sediments, and sewage sludge. It has been detected in plants, paper products, some food items, and even in the human body. In addition, compared to BPA, BPS has a lower acute toxicity, similar or less endocrine disruption, similar neurotoxicity and immunotoxicity, and lower reproductive and developmental toxicity. The mechanisms underlying BPS toxicity may be related to the chemical properties of BPS in the human body, including interactions with estrogen receptors, and binding to DNA and some proteins, subsequently including exerting oxidative stress. However, further investigation on the potential risks of BPS to humans and its mechanisms of toxicity should be conducted to better understand and control the risks of such novel chemicals.

Pharmaceuticals and personal care products in water, sediments, aquatic organisms, and fish feeds in the Pearl River Delta: Occurrence, distribution, potential sources, and health risk assessment.

This study investigated the occurrence, distribution, and potential sources of 34 pharmaceuticals and personal care products (PPCPs) in water, sediments, aquatic organisms (fish and shellfish), and fish feeds from the mariculture areas of the Pearl River Delta (PRD). The health risk presented by this class of compounds was also assessed in relation to their intake via seafood consumption. Of the 34 PPCPs, a total of 9, 21, 14, and 28 PPCPs were detected in water, sediments, fish feeds, and aquatic organisms, respectively. Trimethoprim, norfloxacin, ofloxacin, and spectinomycin were detected in all matrices. The levels of PPCPs in water and sediment samples were relatively low. Spectinomycin, paracetamol, ciprofloxacin, norfloxacin, and ibuprofen were the most frequently detected PPCPs in feeds. Ibuprofen and ketoprofen were widely detected in aquatic organisms, with average concentrations of 562 and 267 ng/g wet weight, respectively. The residual levels of PPCPs in shellfish such as ME (mussel, Mytilus edulis) and OS (oyster, Ostrea gigas) were significantly higher (p < 0.05) than those in other species including CA (topmouth culter, Culter alburnus) and EO (orbfish, Ephippus orbis). Correlation analysis indicated that the medicated feeds were a potential source of PPCPs in the mariculture areas of the PRD, but other anthropogenic sources should not be ignored. Based on maximum residue limits and acceptable daily intake, the health risks presented to humans via seafood consumption are negligible. However, as multiple antibiotics were frequently detected in the mariculture environment, aquatic organisms, and feeds, the induction and dissemination of antimicrobial resistance associated with antibiotic usage in aquaculture would be of great concern. It is necessary to establish a centralized management system and control the use of veterinary drugs in mariculture to protect the aquaculture environment and ensure the safety of seafood.