Nonuniform accumulation of cadmium and copper in kidneys of wild brown trout (Salmo trutta) populations.

Cadmium and copper accumulated nonuniformly in the kidney of wild brown trout on a longitudinal gradient as follows: the concentration in head kidney < anterior portion of tail kidney < posterior portion(s) of tail kidney. Zinc accumulated uniformly along the length of the kidney. Cadmium and copper accumulation in brown trout kidneys was lowest in the head kidney, where hematopoietic tissue dominates, and greatest in posterior tail sections, where filtration tissues dominate. Sampling programs designed to monitor cadmium and copper accumulation in trout kidneys should utilize the entire kidney, including the head kidney, to create comparable data sets.

Phylogeography of the plains killifish, Fundulus zebrinus.

Drainage systems of the Great Plains and western Gulf Slope underwent substantial changes through diversions and stream captures during the Pleistocene, either as the result of the glacial advances or through independent geologic processes. The distributions of a variety of fishes that range across west-central North America, such as the plains killifish (Fundulus zebrinus), are thought to be the product of this Pleistocene influence. We examined the geographic pattern of genetic variation in F. zebrinus using three allozyme loci (n = 793), mitochondrial DNA restriction fragment length polymorphisms (RFLPs, n = 352), and sequencing of the mitochondrial cytochrome oxidase I (COI, n = 23) in an attempt to understand the roles of dispersal and vicariance. The phylogeographic patterns were concordant between the allozyme and mitochondrial data with the exception of the population in the North Canadian River. The populations fell into three geographic assemblages, which we designated as northern, central, and southern. A large phylogenetic break (average Roger's D = 0.702; average sequence divergence in RFLPs = 4.6%; average sequence divergence in COI = 5.5%) separated the northern/central and southern assemblages. The northern region was likely colonized sometime during the mid-Pleistocene. Fish in the Brazos and Pecos Rivers probably reached these drainages through stream captures of the Red River. The large phylogenetic break between the northern/central and southern clades supports previous attempts to recognize two species of plains killifish: F. zebrinus and F. kansae.

Some aspects of hepatic function in feral brown trout, Salmo trutta, living in metal contaminated water.

Brown trout, Salmo trutta, exposed to heavy metals (mainly Cd and Zn) for at least 2 years in the Eagle River, Colorado, were examined for liver size and activity of the growth-promoting enzyme, ornithine decarboxylase (ODC) and compared to trout living in an uncontaminated site. Liver-somatic index (LSI) was greater for trout living in the uncontaminated site with the LSI of females being significantly greater than that of males. The LSI for females at the uncontaminated site was greater than that of females at the contaminated site, but males were not different statistically. ODC activity in the livers of both males and females was lower at the contaminated site. However, males and females did not differ with respect to ODC activity. These data suggest that chronic exposure to heavy metals may have important implications for growth and reproduction and possibly survival. The activity of ODC in liver might serve as a useful biomarker when assessing chronic toxicity of metals to naturally reproducing fish populations.

Impaired adrenocortical response to stress by brown trout, Salmo trutta, living in metal-contaminated waters of the Eagle River, Colorado.

Brown trout, Salmo trutta, were collected from two sites contaminated with cadmium (Cd) and zinc (Zn) and one uncontaminated site. These fish were subjected to a continuous confinement stressor in wire cages placed in the river (moderate stress) or in 5-gal. plastic buckets on land (severe stress). Plasma cortisol and corticotropin (ACTH) were determined for fish in buckets by radioimmunoassay after 0, 1, 3, 12, or 24 h of confinement. Plasma cortisol and ACTH levels of brown trout from both contaminated and uncontaminated sites initially were the same and increased with time. However, the rise in plasma cortisol was delayed significantly in fish residing in contaminated sites, even though ACTH secretion initially was elevated compared with control trout. Furthermore, secretion of cortisol and ACTH by these fish declined significantly between 3 and 24 h of confinement. Fish from the uncontaminated site responded more rapidly to confinement with increased cortisol secretion and elevated levels of ACTH and continued to exhibit elevated levels of both hormones up to 24 h of confinement. Caged fish examined after 0, 3, 12, and 24 h of confinement exhibited similar plasma cortisol responses regardless of previous exposure to metals. These results suggest that the overall response to severe, short-term confinement stress by the hypothalamo-pituitary-adrenocortical axis of fish chronically exposed to Cd and Zn was depressed and that these fish could not sustain the stress response as readily as fish living in uncontaminated water.

Unilateral ovariectomy influences hypothalamic monoamine asymmetries in a lizard (Anolis) that exhibits alternation of ovulation.

The lizard Anolis carolinensis alternates ovulation, and the resultant ovarian asymmetry correlates with alternating asymmetry of hypothalamic catecholamines. Unilateral and bilateral ovariectomies of cycling females were performed to determine if ovarian manipulations influence hypothalamic catecholamine asymmetries. During the middle of the ovarian cycle, we removed the larger ovary, i.e., the next one to ovulate an egg (N = 9), the smaller ovary with its corpus luteum (N = 8), or both ovaries (N = 5). A sham-operated control group was included (N = 9). The diameter of the largest ovarian follicle in each ovary was measured at the beginning and end of the experiment. After 12 days, the hemihypothalami from the sides of the initial smaller ovary (SO) and larger ovary (LO) were dissected and frozen for determination of monoamines and their metabolites using HPLC and electrochemical detection. Monoamine and metabolite concentrations at the end of the experiment in the original SO and LO sides of each hypothalamus were compared with an asymmetry ratio, or AR, of (SO side - LO side)/(SO side + LO side). No female ovulated during the experiment. Unilateral ovariectomy caused compensatory growth of the largest follicle in the remaining ovary. Removal of the SO or LO caused the AR of DOPAC to favor the brain side ipsilateral to that of the ovarian removal. Removal of the LO switched the NE AR from the SO to the LO side. Removal of the LO or SO caused the MHPG AR to favor the LO side. Ovariectomy of any kind caused 5-HT, which in the sham-operated animals favored the SO side, to become symmetric, and removal of the LO caused the 5-HIAA AR to favor the LO side. We conclude that the ovaries influence hypothalamic catecholamine asymmetries in Anolis via direct neural (as well as hormonal) pathways and that sensory input from the ovaries to the hypothalamus could be involved in control of ovarian alternation via both neural and hormonal efferent mechanisms.

Immunocytochemical and histological differences in the interrenal axis of feral brown trout, Salmo trutta, in metal-contaminated waters.

There are more CRH-like immunoreactive neurons in the preoptic nucleus and nucleus lateralis tuberis in the brain of feral brown trout, Salmo trutta, living in cadmium- and zinc-contaminated regions of the Eagle River than in fish from an uncontaminated control site. Histological analyses revealed that interrenal cells are more stimulated (exhibiting both hypertrophy and hyperplasia) in fish living in contaminated sites than interrenal cells of fish at the control site. These results suggest that the hypothalamo-pituitary-interrenal (HPI) axis of fish living in the metal-contaminated water shows evidence of chronic stimulation. We suggest that assessment of these parameters of the HPI axis may be useful indices of chronic environmental stress in trout.