RESUMO
Systematic screening of algal cells is getting huge interest due to their capability of producing lipid-based biodiesel. Here, we introduce a new microfluidic platform composed of an array of perfusion chambers designed for long-term cultivation and preliminary screening of motile microalgal cells through loading and releasing of cells to and from the chambers. The chemical environment in each perfusion chamber was independently controlled for 5 days. The effect of nitrogen-depletion on the lipid production, phototaxis behavior in the absence of Ca(2+), and cytotoxic effect of herbicide on microalgal cells was successfully monitored and compared with simultaneous control experiments on the platform. The present methodology could be extended to effective screening of algal cells and various cell lines for the production of biodiesel and other useful chemicals.
RESUMO
PURPOSE: Although a confocal microscopy has been largely used, there has been no domestic report concerning the normal structure of rabbit and human cornea by confocal microscope. In this study, we investigated confocal microscopic morphology of normal cornea of rabbit and human. MEHTODS: Using confocal microscope (ConfoScan 2.0, Fortune Technology, Italy), cross-sectioned corneal images of the corneal epithelium, Bowman's layer, stromal layer (anterior, middle, and posterior keratocyte), Descemet 's membrane, and endothelium were examined in vivo. RESULTS: In normal rabbit corneas, the superficial epithelium was polygonal and Bowman's layer was wrinkled. The stromal keratocyte nuclei was elliptical anteriorly and was with less cellularity posteriorly. The endothelium of rabbit was uniform, hexagonal in shape and had highly reflective cell borders. In human corneas, the superficial epithelium had highly reflective nuclei and regular wing cell or subepithelial nerve plexus was visible. Bowman's layer was fold-like structure. The shape of anterior and middle keratocyte nuclei were polygonal, but posterior keratocyte was oval shape. Also the nerve plexus of middle stromal layer was visible and the nucleus of endothelial cell was reflective hexagonal shape. CONCLUSIONS: The normal corneal morphological findings were studied by confocal microscopy. These data might provide the useful informations for the diagnosis of corneal disease.
