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1.
J Phys Chem Lett ; 13(35): 8312-8318, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36040023

RESUMO

The pyronin class of fluorophores serves a critical role in numerous imaging applications, particularly involving preferential staining of RNA through base pair intercalation. Despite this important role in molecular staining applications, the same set of century-old pyronins (i.e., pyronin Y (PY) and pyronin B (PB)), which possess relatively low fluorophore brightness, are still predominantly being used due to the lack of methodology for generating enhanced variants. Here, we use TD-DFT calculations of interconversion energies between structures on the S1 surface as a preliminary means to evaluate fluorophore brightness for a proposed set of pyronins containing variable substitution patterns at the 2, 3, 6, and 7 positions. Using a nucleophilic aromatic substitution/hydride addition approach, we synthesized the same set of pyronins and demonstrate that quantum-mechanical computations are useful for predicting fluorophore performance. We produced the brightest series of pyronin fluorophores described to date, which possess considerable gains over PY and PB.


Assuntos
Pironina , RNA , Corantes Fluorescentes , Estrutura Molecular , Pironina/química , Coloração e Rotulagem
2.
RSC Med Chem ; 13(4): 456-462, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35647549

RESUMO

Fluorinated analogues of the fluorophore pyronin B were synthesized as a new class of amine-reactive drug-like small molecules. In water, 2,7-difluoropyronin B was found to reversibly react with primary amines to form covalent adducts. When this fluorinated analogue is added to proteins, these adducts undergo additional oxidation to yield fluorescent 9-aminopyronins. Irradiation with visible blue light enhances this oxidation step, providing a photochemical method to modify the biological properties of reactive amines. In living HeLa cells, 2,7-difluoropyronin B becomes localized in mitochondria, where it is partially transformed into fluorescent aminopyronins, as detected by spectral profiling confocal microscopy. Further excitation of these cells with the blue laser of a confocal microscope can depolarize mitochondria within seconds. This biological activity was only observed with 2,7-difluoropyronin B and was not detected with analogues such as pyronin B or 9-methyl-2,7-difluoropyronin B. This irradiation with blue light enhances the cellular production of reactive oxygen species (ROS), suggesting that increased ROS in mitochondria promotes the formation of aminopyronins that inactivate biomolecules critical for maintenance of mitochondrial membrane potential. The unique reactivity of 2,7-difluoropyronin B offers a novel tool for photochemical control of mitochondrial biology.

3.
J Vis Exp ; (172)2021 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-34180889

RESUMO

Methine-bridged conjugated bicyclic aromatic compounds are common constituents of a range of biologically relevant molecules such as porphyrins, dipyrrinones, and pharmaceuticals. Additionally, restricted rotation of these systems often results in highly to moderately fluorescent systems as observed in 3H,5H-dipyrrolo[1,2-c:2',1'-f]pyrimidin-3-ones, xanthoglows, pyrroloindolizinedione analogs, BODIPY analogs, and the phenolic and imidazolinone ring systems of Green Fluorescent Protein (GFP). This manuscript describes an inexpensive and operationally simple method of performing a Claisen-Schmidt condensation to generate a series of fluorescent pH dependent pyrazole/imidazole/isoindolone dipyrrinone analogs. While the methodology illustrates the synthesis of dipyrrinone analogs, it can be translated to produce a wide range of conjugated bicyclic aromatic compounds. The Claisen-Schmidt condensation reaction utilized in this method is limited in scope to nucleophiles and electrophiles that are enolizable under basic conditions (nucleophile component) and non-enolizable aldehydes (electrophile component). Additionally, both the nucleophilic and electrophilic reactants must contain functional groups that will not inadvertently react with hydroxide. Despite these limitations, this methodology offers access to completely novel systems that can be employed as biological or molecular probes.


Assuntos
Imidazóis , Pirazóis , Concentração de Íons de Hidrogênio , Isoindóis , Estrutura Molecular
4.
ChemMedChem ; 16(5): 839-850, 2021 03 03.
Artigo em Inglês | MEDLINE | ID: mdl-33301228

RESUMO

Ser/Thr protein phosphatases (PPs) regulate a substantial range of cellular processes with protein phosphatases 1 (PP1) and 2 A (PP2A) accounting for over 90 % of the activity within cells. Nevertheless, tools to study PPs are limited as PPs inhibitors, particularly those selective for PP1 inhibition, are relatively scarce. Two examples of PP1-selective inhibitors, which share structural similarities, are tautomycin (TTM) and tautomycetin (TTN). This work describes the development of PP1/PP2A inhibitors that incorporate key structural features of TTM and TTN and are designed to conserve regions known to bind the active site of PP1/PP2A but vary regions that differentially contact the hydrophobic groove of PP1/PP2A. In all 28 TTN analogues were synthetically generated that inhibit PP1/PP2A activity at <250 mM; seven possessed inhibition activity at 100 nM. The IC50 values were determined for the seven most active analogues, which ranged from 34 to 1500 nM (PP1) and 70 to 6800 nM (PP2A). Four of the seven analogues possessed PP1 selectivity, and one demonstrated eightfold selectivity in the nanomolar range (PP1 IC50 =34 nM, PP2A IC50 =270 nM). A rationale is given for the observed differences in selectivity.


Assuntos
Inibidores Enzimáticos/farmacologia , Furanos/farmacologia , Lipídeos/farmacologia , Proteína Fosfatase 2/antagonistas & inibidores , Receptores de Neuropeptídeo Y/antagonistas & inibidores , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Furanos/síntese química , Furanos/química , Humanos , Lipídeos/síntese química , Lipídeos/química , Estrutura Molecular , Proteína Fosfatase 2/metabolismo , Receptores de Neuropeptídeo Y/metabolismo , Relação Estrutura-Atividade
5.
J Org Chem ; 84(18): 11856-11862, 2019 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-31438666

RESUMO

Dipyrrinones are nonfluorescent yellow-pigmented constituents of bilirubin that undergo Z to E isomerization when excited with UV/blue light. Mechanical restriction of the E/Z isomerization process results in highly fluorescent compounds such as N,N-methylene-bridged dipyrrinones and xanthoglows. This manuscript describes the first examples of dipyrrinone analogues, which exhibit fluorescence without covalently linking the pyrole-pyrrolidine nitrogen atoms. Instead these analogues restrict E/Z isomerization through intramolecular hydrogen bonding, resulting in mild to moderately fluorescent compounds (ΦF = 0.01-0.30). Further, in basic solutions (pH > 12), the dipyrrinone analogues readily deprotonate and absorption/emission profiles of the fluorophores red-shifts by 10-49 nm. Directly from commercial materials, 10 analogues were prepared in 41-96% yields in one step. To estimate the capacity of which intramolecular hydrogen bonding has upon restricting the E/Z isomerization process, conformational energies of all analogues, in both the protonated and deprotonated species, were explored by using quantum-mechanical density functional theory (DFT) and time-dependent DFT calculations. The computed strengths of the intramolecular hydrogen bonds are related to the barriers of rotation about the 5-6 bond and both correlate with the experimentally measured fluorescence quantum yields.


Assuntos
Bilirrubina/química , Corantes Fluorescentes/síntese química , Imidazóis/química , Isoindóis/química , Pirazóis/química , Corantes Fluorescentes/química , Corantes Fluorescentes/farmacologia , Concentração de Íons de Hidrogênio , Modelos Moleculares , Estrutura Molecular , Soluções
6.
Synth Commun ; 46(5): 475-481, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27688484

RESUMO

A practical and convenient procedure for the nucleophilic aromatic substitution of aryl fluorides and chlorides with dimethylamine was developed using a hydroxide assisted, thermal decomposition of N,N-dimethylforamide. These conditions are tolerant of nitro, nitrile, aldehyde, ketone, and amide groups but will undergo acyl substitution to form amides for methyl esters and acyl chlorides. Isolated yields of the products range from 44 - 98%, with the majority being greater than 70% for seventeen examples.

7.
J Chem Educ ; 92(7): 1221-1225, 2015 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-27681671

RESUMO

A three-pot synthesis oriented for an undergraduate organic chemistry laboratory was developed to construct a fluorescent acridone molecule. This laboratory experiment utilizes Grignard addition to an aldehyde, alcohol oxidation, and iterative nucleophilic aromatic substitution steps to produce the final product. Each of the intermediates and the acridone product of the synthesis are analyzed by common techniques available in most undergraduate chemistry laboratories, such as melting point, TLC, IR spectroscopy, UV-Vis spectroscopy, and fluorescence spectroscopy. Yields for each transformation in the synthesis are generally moderately low to good (20-90%) and nearly all of the students (>90%) who attempted the synthesis were able to produce the final acridone product.

8.
Synthesis (Stuttg) ; 46(2): 158-164, 2014 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-24914246

RESUMO

Fluorinated fluorophores are valuable tools for studies of biological systems. However, amine-reactive single-isomer derivatives of these compounds are often very expensive. To provide an inexpensive alternative, we report a practical synthesis of 4-carboxy-Pennsylvania Green methyl ester. Derivatives of this hydrophobic fluorinated fluorophore, a hybrid of the dyes Oregon Green and Tokyo Green, are often cell permeable, enabling labeling of intracellular targets and components. Moreover, the low pKa of Pennsylvania Green (4.8) confers bright fluorescence in acidic cellular compartments such as endosomes, enhancing its utility for chemical biology investigations. To improve access to the key intermediate 2,7-difluoro-3,6-dihydroxyxanthen-9-one, we subjected bis-(2,4,5-trifluorophenyl)methanone to iterative nucleophilic aromatic substitution by hydroxide on scales of > 40 g. This intermediate was used to prepare over 15 grams of pure 4-carboxy-Pennsylvania Green methyl ester in 28% overall yield without requiring chromatography. This compound can be converted into the amine reactive N-hydroxysuccinimidyl ester in essentially quantitative yield for the synthesis of a wide variety of fluorescent molecular probes.

9.
J Am Chem Soc ; 136(14): 5241-4, 2014 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-24660775

RESUMO

Critical protein-protein interactions are ubiquitous in biology. To provide a new method to detect these interactions, we designed and synthesized fluorinated bromopyronins as molecular probes. These electrophilic compounds rapidly react with amines via a S(N)Ar mechanism to form modestly electrophilic aminopyronin fluorophores. To investigate whether proteins modified with aminopyronins might selectively transfer these fluorophores between proximal lysine residues at protein-protein interfaces, immunoglobulin-G (IgG) was conjugated to fluorinated pyronins and added to unlabeled Protein A (SpA) from S. aureus. Analysis by gel electrophoresis and mass spectrometry revealed transfer of this fluorophore from IgG to specific lysines of its binding partner SpA but not to bovine serum albumin (BSA) as a nonbinding control. Examination of an X-ray structure of IgG bound to SpA revealed that the fluorophore was selectively transferred between amino groups of lysines that reside within ~10 Å at the protein-protein interface. To evaluate whether this approach could be used to identify interactions with endogenous cellular proteins, pyronin-modified Rnase A was added to crude extracts of human HeLa cells. Analysis of interacting proteins by gel electrophoresis revealed the endogenous ribonuclease inhibitor as the primary cellular target. Given that proximal lysine residues frequently reside at protein-protein interfaces, this method may facilitate identification of diverse protein-protein interactions present in complex biological matrices.


Assuntos
Lisina/química , Cristalografia por Raios X , Imunoglobulina G/química , Modelos Moleculares , Sondas Moleculares/síntese química , Sondas Moleculares/química , Estrutura Molecular , Ligação Proteica , Proteína Estafilocócica A/química
10.
ACS Chem Biol ; 8(3): 636-42, 2013 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-23256594

RESUMO

Unlike the digestive systems of vertebrate animals, the lumen of the alimentary canal of Caenorhabditis elegans is unsegmented and weakly acidic (pH ~4.4), with ultradian fluctuations to pH > 6 every 45-50 s. To probe the dynamics of this acidity, we synthesized novel acid-activated fluorophores termed Kansas Reds. These dicationic derivatives of rhodamine B become concentrated in the lumen of the intestine of living C. elegans and exhibit tunable pKa values (2.3-5.4), controlled by the extent of fluorination of an alkylamine substituent, that allow imaging of a range of acidic fluids in vivo. Fluorescence video microscopy of animals freely feeding on these fluorophores revealed that acidity in the C. elegans intestine is discontinuous; the posterior intestine contains a large acidic segment flanked by a smaller region of higher pH at the posterior-most end. Remarkably, during the defecation motor program, this hot spot of acidity rapidly moves from the posterior intestine to the anterior-most intestine where it becomes localized for up to 7 s every 45-50 s. Studies of pH-insensitive and base-activated fluorophores as well as mutant and transgenic animals revealed that this dynamic wave of acidity requires the proton exchanger PBO-4, does not involve substantial movement of fluid, and likely involves the sequential activation of proton transporters on the apical surface of intestinal cells. Lacking a specific organ that sequesters low pH, C. elegans compartmentalizes acidity by producing of a dynamic hot spot of protons that rhythmically migrates from the posterior to anterior intestine.


Assuntos
Caenorhabditis elegans/metabolismo , Mucosa Intestinal/metabolismo , Prótons , Animais , Corantes Fluorescentes/análise , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/química , Concentração de Íons de Hidrogênio , Microscopia de Fluorescência , Estrutura Molecular
11.
J Org Chem ; 77(1): 473-81, 2012 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-22111869

RESUMO

Fluorination of fluorophores can substantially enhance their photostability and improve spectroscopic properties. To facilitate access to fluorinated fluorophores, bis(2,4,5-trifluorophenyl)methanone was synthesized by treatment of 2,4,5-trifluorobenzaldehyde with a Grignard reagent derived from 1-bromo-2,4,5-trifluorobenzene, followed by oxidation of the resulting benzyl alcohol. This hexafluorobenzophenone was subjected to sequential nucleophilic aromatic substitution reactions, first at one or both of the more reactive 4,4'-fluorines, and second by cyclization through substitution of the less reactive 2,2'-fluorines, using a variety of oxygen, nitrogen, and sulfur nucleophiles, including hydroxide, methoxide, amines, and sulfide. This method yields symmetrical and asymmetrical fluorinated benzophenones, xanthones, acridones, and thioxanthones and provides scalable access to known and novel precursors to fluorinated analogues of fluorescein, rhodamine, and other derivatives. Spectroscopic studies revealed that several of these precursors are highly fluorescent, with tunable absorption and emission spectra, depending on the substituents. This approach should allow access to a wide variety of novel fluorinated fluorophores and related compounds.


Assuntos
Acridonas/química , Acridonas/síntese química , Benzofenonas/química , Benzofenonas/síntese química , Xantonas/química , Xantonas/síntese química , Halogenação , Estrutura Molecular , Estereoisomerismo , Tioxantenos/síntese química , Tioxantenos/química
12.
J Org Chem ; 70(21): 8417-23, 2005 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-16209586

RESUMO

A new class of highly fluorescent (phi(F) 0.3-0.8) low molecular weight water-soluble cholephilic compounds has been synthesized in two steps from dipyrrinones. The dipyrrinone nitrogens are first bridged by reaction with 1,1'-carbonyldiimidazole to form an N,N'-carbonyldipyrrinone (3H,5H-dipyrrolo[1,2-c:2',1'-f]pyrimidine-3,5-dione) nucleus, and a sulfonic acid group is then introduced at C(8) by reaction with concd H(2)SO(4). The resulting sulfonated N,N'-carbonyl-bridged dipyrrinones ("sulfoglows") are isolated as their sodium salts. When the alkyl substituents of the lactam ring are lengthened from ethyl to decyl, sulfoglows become increasingly lipophilic while maintaining water solubility. Low molecular weight sulfoglows were rapidly excreted intact in both bile and urine after intravenous infusion into rats, but higher molecular weight sulfoglows were excreted more selectively in bile. Hepatobiliary excretion of sulfoglows was partially, but not completely, blocked in mutant rats deficient in the multidrug-resistance associated transport protein Mrp2 (ABCC2). These observations point to the feasibility of developing simple sulfoglows with clinical diagnostic potential that are normally excreted in bile but appear in urine when hepatic elimination is impaired by cholestatic liver disease.


Assuntos
Colchicina/análise , Corantes Fluorescentes/síntese química , Hepatopatias/diagnóstico , Fígado/metabolismo , Pirimidinonas/síntese química , Pirimidinonas/farmacocinética , Pirróis/síntese química , Pirróis/farmacocinética , Ácidos Sulfônicos/síntese química , Ácidos Sulfônicos/farmacocinética , Animais , Bile/química , Bile/metabolismo , Bilirrubina/análogos & derivados , Bilirrubina/química , Colchicina/química , Avaliação Pré-Clínica de Medicamentos , Estudos de Viabilidade , Corantes Fluorescentes/química , Fígado/efeitos dos fármacos , Hepatopatias/genética , Hepatopatias/metabolismo , Masculino , Proteínas Mitocondriais/deficiência , Proteínas Mitocondriais/genética , Pirimidinonas/análise , Pirróis/análise , Pirróis/química , Ratos , Ratos Gunn , Ratos Sprague-Dawley , Proteínas Ribossômicas/deficiência , Proteínas Ribossômicas/genética , Proteínas de Saccharomyces cerevisiae/genética , Espectrometria de Fluorescência , Ácidos Sulfônicos/análise , Urina/química
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