RESUMO
OBJECTIVE: To prepare (-)-Antofine liposomes with high activity and good water-soluble and study its anti-breast cancer activity both in vitro and in vivo. METHODS: (-)-Antofine liposomes were prepared by film dispersion method, the encapsulation efficiency of (-)-Antofine liposomes were determined by UV spectrophotometer, the cytotoxic activity of (-)-Antofine liposomes on MCF-7 cell was measured by MTT assay, the antitumor activity and major organs indexes were evaluated by tumor-bearing nude mice experimental in vivo. RESULTS: The average encapsulation efficiency of (-)-Antofine liposomes was (80.8 +/- 2.2)%, average drug loading was (26.7 +/- 1.4)%, it was relatively good water-soluble and had good anti-tumor activity in vitro and in vivo. CONCLUSION: (-)-Antofine liposomes prepared by film dispersion method has high encapsulation efficiency, the water-soluble and the anti-tumor activity are improved compared with (-)-Antofine.
