Effect of compost amendment and bioaugmentation on PAH degradation and microbial community shifting in petroleum-contaminated soil.

Efficient degradation of polycyclic aromatic hydrocarbons (PAHs) in a petroleum-contaminated soil was challenging which requires ample PAH-degrading flora and nutrients. In this study, we investigated the effects of 'natural attenuation', 'bioaugmentation', 'compost only (raw materials of compost included pig manure and rice husk mixed at a 1:2 proportion, supplemented with 2.5% charcoal)', and 'compost with bioaugmentation' treatments on degradation of polycyclic aromatic hydrocarbons (PAHs) and microbial community shifts during the remediation of petroleum-contaminated soil. After sixteen weeks of incubation, the removal efficiencies of PAHs were 0.52 ± 0.04%, 6.92 ± 0. 32%, 9.53 ± 0.29%, and 18.2 ± 0.64% in the four treatments, respectively. 'Compost with bioaugmentation' was the most effective for PAH removal among all the treatments. Illumina sequencing analysis suggested that both the 'compost only' and 'compost with bioaugmentation' treatments changed soil microbial community structures and enhanced microbial biodiversity. Some of the microorganisms affiliated with the compost including Azomonas, Luteimonas, Pseudosphingobacterium, and Parapedobacter were able to survive and become dominant in the contaminated soil. The 'bioaugmentation and 'natural attenuation' treatments had no significant effects on soil microbial community structure. Inoculation of the PAH degraders including Bacillus, Pseudomonas, and Acinetobacter directly into the contaminated soil led to lower biodiversity under natural conditions. This result suggested that compost addition increased the α-diversity of both the bacterial and fungal communities in petroleum-contaminated soil, leading to higher PAH degradation efficiency in petroleum-contaminated soil.

Distribution Characteristics of Bacterial Communities and Hydrocarbon Degradation Dynamics During the Remediation of Petroleum-Contaminated Soil by Enhancing Moisture Content.

Microorganisms are the driver of petroleum hydrocarbon degradation in soil micro-ecological systems. However, the distribution characteristics of microbial communities and hydrocarbon degradation dynamics during the remediation of petroleum-contaminated soil by enhancing moisture content are not clear. In this study, polymerase chain reaction and high-throughput sequencing of soil microbial DNA were applied to investigate the compositions of microorganisms and alpha diversity in the oil-polluted soil, and the hydrocarbon removal also being analyzed using ultrasonic extraction and gravimetric method in a laboratory simulated ex-situ experiment. Results showed the distribution of petroleum hydrocarbon degrading microorganisms in the petroleum-contaminated loessal soil mainly was Proteobacteria phylum (96.26%)-Gamma-proteobacteria class (90.03%)-Pseudomonadales order (89.98%)-Pseudomonadaceae family (89.96%)-Pseudomonas sp. (87.22%). After 15% moisture content treatment, Actinobacteria, Proteobacteria, and Firmicutes still were the predominant phyla, but their relative abundances changed greatly. Also Bacillus sp. and Promicromonospora sp. became the predominant genera. Maintaining 15% moisture content increased the relative abundance of Firmicutes phylum and Bacillus sp. As the moisture-treated time increases, the uniformity and the richness of the soil bacterial community were decreased and increased respectively; the relative abundance of Pseudomonas sp. increased. Petroleum hydrocarbon degradation by enhancing soil moisture accorded with the pseudo-first-order reaction kinetic model (correlation coefficient of 0.81; half-life of 56 weeks). The richness of Firmicutes phylum and Bacillus sp. may be a main reason for promoting the removal of 18% petroleum hydrocarbons responded to 15% moisture treatment. Our results provided some beneficial microbiological information of oil-contaminated soil and will promote the exploration of remediation by changing soil moisture content for increasing petroleum hydrocarbon degradation efficiency.

Effect of bioaugmentation and biostimulation on hydrocarbon degradation and microbial community composition in petroleum-contaminated loessal soil.

This study assessed the benefits of biostimulation with nitrogen and phosphorous (BS) versus bioaugmentation with native petroleum degrading flora (BA) in terms of petroleum hydrocarbon removal and microbial community structure shift in petroleum-polluted loessal soil. After 12 weeks of remediation, the TPH degradation efficiencies were 28.3% and 13.9% in BS and BA treated soils, respectively. Biostimulation was more effective than bioaugmentation for petroleum hydrocarbon degradation. Soil microbial community composition changed while microbial diversity decreased greatly by bioaugmentation treatment. The inoculum could survive, grow up quickly and become the predominant microorganisms after one week of inoculation. In the biostimulation treatment, microbial community composition is more evenness and richness than in the bioaugmented remediation. The strong positive correlations of the nitrogen and phosphorus with the petroleum hydrocarbon suggest the importance of nutrients for petroleum biodegradation in the contaminated loessal soil. The results indicate that the stabilization and variety of the microbial community structure are essential for the petroleum biodegradation performance. Further engineering is suggested to improve the evenness and richness of the soil microbial community since an abundance of nitrogen and phosphorus nutrients ensures the degraders' activity in the petroleum polluted soil.