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1.
Mar Drugs ; 22(6)2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38921596

RESUMO

Omega-3 fatty acids are in high demand due to their efficacy in treating hypertriglyceridemia and preventing cardiovascular diseases. However, the growth of the industry is hampered by low purity and insufficient productivity. This study aims to develop an efficient RP-MPLC purification method for omega-3 fatty acid ethyl esters with high purity and capacity. The results indicate that the AQ-C18 featuring polar end-capped silanol groups outperformed C18 and others in retention time and impurity separation. By injecting pure fish oil esters with a volume equivalent to a 1.25% bed volume on an AQ-C18 MPLC column using a binary isocratic methanol-water (90:10, v:v) mobile phase at 30 mL/min, optimal omega-3 fatty acid ethyl esters were obtained, with the notable purity of 90.34% and a recovery rate of 74.30%. The total content of EPA and DHA produced increased from 67.91% to 85.27%, meeting the acceptance criteria of no less than 84% set by the 2020 edition of the Pharmacopoeia of the People's Republic of China. In contrast, RP-MPLC significantly enhanced the production efficiency per unit output compared to RP-HPLC. This study demonstrates a pioneering approach to producing omega-3 fatty acid ethyl esters with high purity and of greater quantity using AQ-C18 RP-MPLC, showing this method's significant potential for use in industrial-scale manufacturing.


Assuntos
Cromatografia de Fase Reversa , Ésteres , Ácidos Graxos Ômega-3 , Óleos de Peixe , Ácidos Graxos Ômega-3/química , Ácidos Graxos Ômega-3/isolamento & purificação , Ésteres/química , Ésteres/isolamento & purificação , Óleos de Peixe/química , Cromatografia de Fase Reversa/métodos , Cromatografia Líquida de Alta Pressão/métodos , Ácidos Docosa-Hexaenoicos/química , Ácidos Docosa-Hexaenoicos/isolamento & purificação , Ácido Eicosapentaenoico/química , Ácido Eicosapentaenoico/isolamento & purificação
2.
Curr Res Food Sci ; 7: 100624, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37954914

RESUMO

Bangia fusco-purpurea is an economically important seaweed with Fujian characteristics. Given that its harvest is seasonal, drying is often used to remove moisture, extend storage time, and facilitate further processing. Hence, the current study sought to explore the impact of different drying processes on the quality and volatile fingerprints of Bangia fusco-purpurea. To this end, the effects of hot air drying (HAD) and vacuum freeze drying (VFD) on the drying characteristics, microstructure, rehydration, and volatile components of dried B. fusco-purpurea were investigated. The results showed that the water removal efficiency of HAD was significantly higher than that of VFD. However, VFD better preserved the skeletal structure of B. fusco-purpurea than HAD, with a faster rehydration rate and a more uniform cell structure after rehydration. Using electronic nose and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF MS), significant differences in the volatile profiles of fresh, HAD, and VFD B. fusco-purpurea were assessed. E-nose analysis revealed that both HAD and VFD treatments significantly reduced sulfides, aromatic compounds, and nitrogen oxides in fresh B. fusco-purpurea. However, the alcohol, aldehyde, and ketone contents were lower in the VFD samples compared with HAD and fresh samples, whereas the content of methyl flavor substances was significantly higher. GC × GC-TOF MS analysis revealed that the most abundant volatile categories in HAD and VFD were hydrocarbons, alcohols, and esters. The number of volatile components in the HAD samples was significantly lower than in the VFD and fresh samples. As drying progressed, hydrocarbons and alcohols were formed in dried B. fusco-purpurea due to the thermal degradation of carbohydrates, lipids, amino acids, and the Maillard reaction. There were also significant flavor differences between HAD and VFD B. fusco-purpurea. Thus, although HAD exhibits better drying efficiency, VFD has more significant advantages in terms of product quality.

3.
Front Genet ; 13: 958092, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36061171

RESUMO

Background: Ovarian cancer (OC) is a highly heterogeneous disease, of which the mesenchymal subtype has the worst prognosis, is the most aggressive, and has the highest drug resistance. The cell cycle pathway plays a vital role in ovarian cancer development and progression. We aimed to screen the key cell cycle genes that regulated the mesenchymal subtype and construct a robust signature for ovarian cancer risk stratification. Methods: Network inference was conducted by integrating the differentially expressed cell cycle signature genes and target genes between the mesenchymal and non-mesenchymal subtypes of ovarian cancer and identifying the dominant cell cycle signature genes. Results: Network analysis revealed that two cell cycle signature genes (POLA2 and KIF20B) predominantly regulated the mesenchymal modalities of OC and used to construct a prognostic model, termed the Cell Cycle Prognostic Signature of Ovarian Cancer (CCPOC). The CCPOC-high patients showed an unfavorable prognosis in the GSE26712 cohort, consistent with the results in the seven public validation cohorts and one independent internal cohort (BL-OC cohort, qRT-PCR, n = 51). Functional analysis, drug-sensitive analysis, and survival analysis showed that CCPOC-low patients were related to strengthened tumor immunogenicity and sensitive to the anti-PD-1/PD-L1 response rate in pan-cancer (r = -0.47, OC excluded), which indicated that CCPOC-low patients may be more sensitive to anti-PD-1/PD-L1. Conclusion: We constructed and validated a subtype-specific, cell cycle-based prognostic signature for ovarian cancer, which has great potential for predicting the response of anti-PD-1/PD-L1.

4.
Mar Drugs ; 20(9)2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36135764

RESUMO

Four undescribed phenolic compounds, namely asperpropanols A-D (1-4), along with two known congeners 5 and 6, were isolated from Aspergillus puniceus A2, a deep-sea-derived fungus. The gross structures of the compounds were established by detailed analyses of the HRESIMS and NMR data, and their absolute configurations were resolved by modified Mosher's method and calculations of ECD data. Compounds 1-6 were found to have excellent anti-inflammatory effect on lipopolysaccharide (LPS)-induced RAW264.7 cells at 20 µM, evidenced by the reduced nitric oxide (NO), tumor necrosis factor α, and interleukin 6 production. Among them, 5 and 6 showed inhibitory effects on NO production comparable with the positive control (BAY11-7083 at 10 µM). Additionally, the LPS-induced mRNA expressions of inducible nitric oxide synthase and cyclooxygenase-2 were also decreased. Interestingly, mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) was downregulated by LPS and recovered by 1-6, suggesting a vital role of Nrf2 in their effect. We further found that pharmacological inhibition of Nrf2 by ML385 largely abrogated the effects of 1-6 on RAW264.7 cells. Therefore, 1-6 may share a common anti-inflammatory mechanism via Nrf2 upregulation and activation.


Assuntos
Lipopolissacarídeos , Fator 2 Relacionado a NF-E2 , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Aspergillus , Ciclo-Oxigenase 2/metabolismo , Fungos/química , Heme Oxigenase-1/metabolismo , Interleucina-6/metabolismo , Lipopolissacarídeos/farmacologia , Fator 2 Relacionado a NF-E2/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Fenóis/farmacologia , RNA Mensageiro , Fator de Necrose Tumoral alfa/metabolismo
5.
Front Bioeng Biotechnol ; 10: 908033, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35832410

RESUMO

Ultraviolet B (UVB) radiation leads to the excessive accumulation of reactive oxygen species (ROS), which subsequently promote inflammation, degradation of the extracellular matrix, and photoaging in skin. Thus antioxidant activity is particularly important when screening for active substances to prevent or repair photodamage. Marine fish-derived bioactive peptides have become a trend in cosmetics and functional food industries owing to their potential dermatological benefits. In this study, 1-diphenyl- 2-pycryl-hydrazyl (DPPH) scavenging activity was selected to optimize the hydrolysis conditions of sturgeon skin collagen peptides with antioxidant activity. The optimal hydrolysis conditions for sturgeon skin collagen hydrolysate (SSCH) were determined by response surface methodology, which comprised an enzyme dosage of flavorzyme at 6,068.4 U/g, temperature of 35.5°C, pH of 7, and hydrolysis time of 6 h. SSCH showed good radical-scavenging capacities with a DPPH scavenging efficiency of 95%. Then, the effect of low-molecular-weight SSCH fraction (SSCH-L) on UVB irradiation-induced photodamage was evaluated in mouse fibroblast L929 cells and zebrafish. SSCH-L reduced intracellular ROS levels and the malondialdehyde content, thereby alleviating the oxidative damage caused by UVB radiation. Moreover SSCH-L inhibited the mRNA expression of genes encoding the pro-inflammatory cytokines IL-1ß, IL-6, TNF-α, and Cox-2. SSCH-L treatment further increased the collagen Ⅰα1 content and had a significant inhibitory effect on matrix metalloproteinase expression. The phosphorylation level of JNK and the expression of c-Jun protein were significantly reduced by SSCH-L. Additionally, SSCH-L increased the tail fin area at 0.125 and 0.25 mg/ml in a zebrafish UVB radiation model, which highlighted the potential of SSCH-L to repair UVB-irradiated zebrafish skin damage. Peptide sequences of SSCH-L were identified by liquid chromatography-tandem mass spectrometry. Based on the 3D-QSAR modeling prediction, six total peptides were selected to test the UVB-protective activity. Among these peptides, DPFRHY showed good UVB-repair activity, ROS-scavenging activity, DNA damage-protective activity and apoptosis inhibition activity. These results suggested that DPFRHY has potential applications as a natural anti-photodamage material in cosmetic and functional food industries.

6.
BMC Complement Med Ther ; 22(1): 144, 2022 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-35597942

RESUMO

BACKGROUND: Chronic exposure to ultraviolet B (UVB) causes a series of adverse skin reactions, such as erythema, sunburn, photoaging, and cancer, by altering signaling pathways related to inflammation, oxidative stress, and DNA damage. Marine algae have abundant amounts and varieties of bioactive compounds that possess antioxidant and anti-inflammatory properties. Thus, the objective of this study was to investigate the photoprotective effects of an ethanol extract of Sargassum thunbergii. METHODS: Sargassum thunbergii phenolic-rich extract (STPE) was prepared, and its activity against UVB damage was evaluated using L929 fibroblast cells and zebrafish. STPE was extracted and purified by 40% ethanol and macroporous resin XDA-7. Reactive oxygen species (ROS) and antioxidant markers, such as superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde (MDA) content were analyzed. The effect of STPE on UVB-induced inflammation was determined by inflammatory cytokine gene and protein expression. The expression of signaling molecules in the Nuclear Factor KappaB (NF-κB) pathway was determined by western blotting. DNA condensation was analyzed and visualized by Hoechst 33342 staining. In vivo evaluation was performed by tail fin area and ROS measurement using the zebrafish model. RESULTS: The total polyphenol content of STPE was 72%. STPE reduced ROS content in L929 cells, improved SOD and CAT activities, and significantly reduced MDA content, thereby effectively alleviating UVB radiation-induced oxidative damage. STPE inhibited the mRNA and protein expression of TNF-α, IL-6, and IL-1α. STPE reversed DNA condensation at concentrations of 20 and 40 µg/mL compared with the UVB control. Moreover, STPE inhibited NF-κB signaling pathway activation and alleviated DNA agglutination in L929 cells after UVB irradiation. Additionally, 1.67 µg/mL STPE significantly increased the tail fin area in zebrafish, and 0.8-1.6 µg/mL STPE effectively eliminated excessive ROS after UVB radiation. CONCLUSIONS: STPE inhibited UVB-induced oxidative stress, inflammatory cytokine expression, and DNA condensation via the downregulation of the NF-κB signaling pathway, suggesting that it prevents UVB-induced photodamage, and has potential for clinical development for skin disease treatment.


Assuntos
Sargassum , Animais , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Citocinas/metabolismo , Etanol , Fibroblastos , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Camundongos , NF-kappa B/metabolismo , Extratos Vegetais/metabolismo , Extratos Vegetais/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Sargassum/metabolismo , Superóxido Dismutase/metabolismo , Raios Ultravioleta/efeitos adversos , Peixe-Zebra/metabolismo
7.
Food Sci Nutr ; 9(12): 6707-6719, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34925800

RESUMO

Although ovarian cancer is common, its prognosis remains poor because of drug resistance and early metastasis. Polysaccharides extracted from Bangia fuscopurpurea (BFP) are potential anti-cancer agents, but the mechanisms underlying their effects in human ovarian cancer remain unclear. Here, we investigated the mechanisms of action of BFP polysaccharides in A2780 ovarian cancer cells using cell migration, invasion, apoptosis, and autophagy assays. Transwell assays indicated that BFP inhibited cell migration and invasion. Flow cytometry analysis showed that BFP treatment induced apoptosis and reactive oxygen species production, while significantly reducing mitochondrial membrane potential. Reverse transcription-polymerase chain reaction and Western blot analyses revealed changes in the expression of apoptosis- and autophagy-related cellular mRNAs and proteins, respectively, following BFP treatment for 24 h. Transmission electron microscopy revealed that BFP induced autophagy in A2780 cells. These findings demonstrate that BFP may be useful for developing functional foods for cancer therapy.

8.
Int J Gen Med ; 14: 7371-7380, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34744450

RESUMO

PURPOSE: Although the burden of breast cancer remains especially high in rural China, data on the clinicopathological characteristics and prevalence of the breast cancer susceptibility gene 1/2 (BRCA1/2) mutations in patients with breast cancer remain limited. We investigated the clinicopathological characteristics, changing patterns, and prevalence of BRCA1/2 mutations in patients with breast cancer. PATIENTS AND METHODS: The clinicopathological characteristics of 3712 women with pathologically confirmed primary breast cancer treated at Meizhou People's Hospital between January 2005 and December 2018 were evaluated. The prevalence of BRCA1/2 mutations in 340 patients with breast cancer diagnosed between January 2017 and September 2018 was also evaluated. RESULTS: The median age at diagnosis was 49±10.5 (range, 20-94) years. Positivity for estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) was observed in 59.0%, 52.5%, and 24.9% of patients, respectively. Time trend analysis revealed that an increasing trend was observed for age at diagnosis (p = 0.001), proportion of patients without a reproductive history (p < 0.001), postmenopausal patients (p = 0.001), invasive pathological cancer type (p = 0.008), ER-positive rate (p < 0.001), PR-positive rate (p = 0.008), and HER2-positive rate (p < 0.001). Compared with patients without BRCA1/2 mutations, those with BRCA1/2 mutations were more likely to have a family history of breast or ovarian cancer (p < 0.001) and have triple-negative breast cancer (TNBC) (p < 0.001). Family history of breast or ovarian cancer (odds ratio [OR], 103.58; 95% confidence interval [CI], 20.58-521.45; p < 0.001) and TNBC subtype (OR, 5.97; 95% CI, 1.16-30.90; p = 0.033) were independent predictors for BRCA1/2 mutation. CONCLUSION: The clinicopathological characteristics of patients with breast cancer in this rural area have changed during the past decade. BRCA1/2 testing should be performed in patients with breast cancer with a family history of breast or ovarian cancer and TNBC.

9.
J Cell Mol Med ; 25(17): 8338-8351, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34302428

RESUMO

A novel polysaccharide was extracted from Sipunculus nudus (SNP). The molecular weight (MW) of SNP was determined to be 9223 Da by high-performance gel permeation chromatography analyses, and the structure of the SNP repeat units was determined to be →3,4-ß-D-GlcpNAC (1→ and →4) -α-D-Glcp (1→ in the ratio of 15:1; →2) -α -D-Galp - (1→ as a side chain; and ß-D-Galp-(1→ and α- D-Glcp - (1→ as end groups by GC-MS analysis and NMR assays. The effect of SNP on hepatoma HepG2-bearing mice was analysed to verify its potential in the clinical treatment of liver cancer. A total of 90 male athymic nu/nu mice were divided into therapeutic and preventive groups and fed with different amounts of SNP. The antitumour effect of SNP on HepG2-bearing mice and mechanism of such were studied by analysing the tumour size, spleen index, thymus index, immune factors in the blood, tumour apoptosis factors, etc. The results suggest that SNP not only increased the index of immune organs in the body, but also enhanced the secretion of immune factors, including interleukin-2, interferon gamma and tumour necrosis factor-alpha in the serum. SNP induced the apoptosis of tumour cells via the mitochondrial apoptosis pathway, which upregulated caspase-3, caspase-8, caspase-9 and BCL2-associated X, but downregulated B-cell lymphoma-2 and vascular endothelial growth factor protein expression. In conclusion, SNP inhibited tumour growth by enhancing immune function and inducing tumour cell apoptosis in HepG2-bearing mice. Therefore, SNP may be further investigated as a promising candidate for future antitumour drugs.


Assuntos
Antineoplásicos/farmacologia , Carcinoma Hepatocelular/tratamento farmacológico , Fatores Imunológicos/farmacologia , Neoplasias Hepáticas/tratamento farmacológico , Nematoides/metabolismo , Polissacarídeos/farmacologia , Animais , Apoptose/efeitos dos fármacos , Células Hep G2 , Humanos , Masculino , Camundongos , Camundongos Nus
10.
Life Sci ; 258: 118176, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32771556

RESUMO

AIMS: We investigated the anti-inflammatory activity of 3ß-hydroxycholest-5-en-7-one from Hippocampus trimaculatus leach and provided a theoretical basis for identifying its therapeutic targets. MAIN METHODS: Small-RNA libraries were constructed for untreated control RAW 264.7 cells and cells treated with lipopolysaccharide (LPS; 1.0 µg/mL) or 10 µM 3ß-hydroxycholest-5-en-7-one +1.0 µg/mL LPS. We constructed and tested a miR-98-5p-interfering lentivirus to evaluate the role of miR-98-5p in the 3ß-hydroxycholest-5-en-7-one-dependent regulation of inflammatory responses in LPS-induced macrophage and murine inflammation models. The small-RNA libraries were analyzed using high-throughput sequencing. KEY FINDINGS: Among the differentially expressed microRNAs, miR-98-5p showed the most significant difference. Bioinformatics tools were used to identify the potential regulatory targets of miR-98-5p, which were tested using dual-luciferase reporter assays. Our results demonstrated that 3ß-hydroxycholest-5-en-7-one exerted an anti-inflammatory effect via miR-98-5p, which negatively regulated the expression of its target gene TNFAIP3. The results indicate that miR-98-5p interference and 3ß-hydroxycholest-5-en-7-one treatment significantly upregulated the low TNFAIP3 expression induced by LPS stimulation, thereby inhibiting TRAF6, RIP, NF-κB, IL-1ß, and TNF-α secretion. SIGNIFICANCE: 3ß-Hydroxycholest-5-en-7-one alleviates inflammation by downregulating miR-98-5p and upregulating TNFAIP3, thereby blocking NF-κB pathway activation. These results reveal the specific anti-inflammatory mechanism of 3ß-hydroxycholest-5-en-7-one, providing a foundation for developing new drugs and identifying drug targets.


Assuntos
Colestenonas/farmacologia , Regulação para Baixo/genética , Inflamação/patologia , MicroRNAs/metabolismo , Smegmamorpha/metabolismo , Regiões 3' não Traduzidas/genética , Animais , Colestenonas/química , Regulação para Baixo/efeitos dos fármacos , Genes Reporter , Inflamação/genética , Lentivirus/metabolismo , Lipopolissacarídeos , Luciferases/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , MicroRNAs/genética , Células RAW 264.7 , Reprodutibilidade dos Testes
11.
World J Microbiol Biotechnol ; 36(8): 115, 2020 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-32661581

RESUMO

Superoxide dismutase (SOD) is an acidic metalloenzyme that scavenges free radicals produced by endogenous and exogenous substances. In the present study, the tissue distribution of the superoxide dismutase HdhCu/Zn-SOD was investigated in Haliotis discus hannai Ino. The expression profile after lipopolysaccharide (LPS) challenge was determined using quantitative real-time polymerase chain reaction (qPCR). To study the antioxidant activity of a recombinant HdhCu/Zn-SOD protein, the HdhCu/Zn-SOD gene was cloned into the pPIC9K vector and transformed into the Pichia pastoris GS115 strain by electroporation. After induction by methanol, the recombinant product was purified using immobilized metal affinity chromatography and confirmed using mass spectrometry. The optimal expression conditions were determined to be incubation with 0.5% methanol at pH 6.0, resulting in a stable expressed product with the molecular weight of approximately 17 kDa and 21 kDa. The enzymatic activity of HdhCu/Zn-SOD consistently increased with increasing Cu2+ concentrations and showed good thermal stability. Recombinant HdhCu/Zn-SOD showed a strong ability to scavenge superoxide anions and hydroxyl radicals and protected L929 cells against the toxicity caused by H2O2 through its in vitro antioxidant activity. The heterologous expression of HdhCu/Zn-SOD in P. pastoris and the antioxidant activity of this enzyme are reported for the first time.


Assuntos
Antioxidantes/metabolismo , Gastrópodes/genética , Superóxido Dismutase/genética , Animais , Gastrópodes/metabolismo , Vetores Genéticos/genética , Vetores Genéticos/metabolismo , Peróxido de Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Saccharomycetales/genética , Saccharomycetales/metabolismo , Superóxido Dismutase/metabolismo , Transcriptoma
12.
J Healthc Eng ; 2020: 6630885, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33489058

RESUMO

Bed resources are the platform in which most medical and health resources in the hospital play a role and carry the core functions of the health service system. How to improve the efficiency of the use of bed resources through scientific management measures and methods and ultimately achieve the optimization of overall health resources is the focus of hospital management teams. This paper analyzes the previous research models of knowledge related to queuing theory in medical services. From the perspective of the hospital and the patient, several indicators such as the average total number of people, the utilization rate of bed resources, the patient stop rate, and the patient average waiting time are defined to measure the performance of the triage queue calling model, which makes the patient queue more reasonable. According to the actual task requirements of a hospital, a Markov queuing strategy based on Markov service is proposed. A mathematical queuing model is constructed, and the process of solving steady-state probability based on Markov theory is analyzed. Through the comparative analysis of simulation experiments, the advantages and disadvantages of the service Markov queuing model and the applicable scope are obtained. Based on the theory of the queuing method, a queuing network model of bed resource allocation is established in principle. Experimental results show that the queuing strategy of bed resource allocation based on Markov optimization effectively improves resource utilization and patient satisfaction and can well meet the individual needs of different patients. It does not only provide specific optimization measures for the object of empirical research but also provides a reference for the development of hospital bed resource allocation in theory.


Assuntos
Alocação de Recursos , Software , Simulação por Computador , Hospitais , Humanos , Teoria de Sistemas
13.
Nat Prod Res ; 32(11): 1329-1332, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28649857

RESUMO

The water-soluble polysaccharides extracted from Sipunculus nudus (SNP) was investigated on the lifespan and immune damage repair of Drosophila melanogaster exposed to Cd (VI). SNP increased superoxyde dismutase (SOD), nitrogen monoxide (NO), glutathione peroxidase (GSH-Px) and total anti-oxidation competence (T-AOC), with decreased malondialdehyde (MDA) on D. melanogaster demonstrated that SNP could attenuate oxidative damage of D. melanogaster Exposed to Cd (VI). Real-time PCR and western blot analysis showed that SNP enhanced the gene expression of Diptericin, Drosomycin, Defensin, PGRP-LC and the protein level of Toll, p-JNK and Relish, that suggested the promoting effect of SNP on the immune damage repair of D. melanogaster exposed to Cd (VI). The increased level of Indy, Parkin and AMPK indicated the regulated effect of SNP on the longevity-related pathways through ageing-related moleculars of D. melanogaster exposed to Cd (VI). These results suggested that SNP could also improve the lifespan of D. melanogaster exposed to Cd (VI).


Assuntos
Cádmio/toxicidade , Drosophila melanogaster/efeitos dos fármacos , Nematoides/química , Polissacarídeos/farmacologia , Animais , Transportadores de Ácidos Dicarboxílicos/genética , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/imunologia , Drosophila melanogaster/fisiologia , Regulação da Expressão Gênica/efeitos dos fármacos , Glutationa Peroxidase/metabolismo , Longevidade/efeitos dos fármacos , Malondialdeído/metabolismo , Polissacarídeos/isolamento & purificação , Superóxido Dismutase/metabolismo , Simportadores/genética , Ubiquitina-Proteína Ligases/genética
14.
Food Funct ; 8(2): 788-795, 2017 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-28119977

RESUMO

Hippocampus trimaculatus leach has been widely used in beverage and herbal medicine fabrication. However, the study of the molecular mechanism of its bioactivity especially the anti-inflammatory activity is still scanty. In the present study, the pure HEO isolated from the dried Hippocampus trimaculatus leach was firstly found to have anti-inflammatory activity in vitro. The molecular mechanism of the anti-inflammatory activity was detailed using the lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cells, suggesting that the HEO can significantly suppress the inflammatory factors of nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α) and interleukin (1L)-1ß (1L-1ß). Cellular signaling analyses demonstrated that the HEO downregulated the NF-κB and extracellular signal-regulated kinase (ERK) of mitogen-activated protein kinase (MAPK) signaling pathways. All the results suggested that the HEO is a potential natural anti-inflammatory agent.


Assuntos
Anti-Inflamatórios/farmacologia , Colestenonas/farmacologia , Interleucina-1beta/imunologia , Macrófagos/efeitos dos fármacos , NF-kappa B/imunologia , Óxido Nítrico Sintase Tipo II/imunologia , Smegmamorpha , Fator de Necrose Tumoral alfa/imunologia , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Colestenonas/química , Colestenonas/isolamento & purificação , Interleucina-1beta/genética , Lipopolissacarídeos/imunologia , Macrófagos/imunologia , Camundongos , Estrutura Molecular , NF-kappa B/genética , Óxido Nítrico Sintase Tipo II/genética , Células RAW 264.7 , Fator de Necrose Tumoral alfa/genética
15.
Int J Biol Macromol ; 87: 597-602, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26987430

RESUMO

Many polysaccharides have biological activities and have been investigated for their antitumor effects. In this study, we investigated the anti-tumor activity and anti-virus activity of SNP-the water-soluble polysaccharides extracted from Sipunculus nudus on Hepg2.2.15. Flow cytometry analysis demonstrated that SNP induced dose-dependent cell apoptosis on Hepg2.2.15. Real-time PCR and Western Blot analysis showed that SNP down-regulated the synthesis of HBsAg, HBV-DNA and enhanced the expression of pro-apoptosis proteins TNF-α, caspase-3, and Bax, while decreasing the expression of the anti-apoptosis proteins survivin, Bcl-2, and VEGF. These results suggested that SNP suppressed cell viability of Hepg2.2.15 and that could be a novel anti-tumor and anti-HBV agent.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Antivirais/química , Antivirais/farmacologia , Organismos Aquáticos/química , Polissacarídeos/farmacologia , Antineoplásicos/isolamento & purificação , Antivirais/isolamento & purificação , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , DNA Viral/genética , Regulação Viral da Expressão Gênica/efeitos dos fármacos , Células Hep G2 , Antígenos de Superfície da Hepatite B/biossíntese , Vírus da Hepatite B/efeitos dos fármacos , Vírus da Hepatite B/genética , Vírus da Hepatite B/metabolismo , Humanos , Proteínas Inibidoras de Apoptose/metabolismo , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Survivina , Fator de Necrose Tumoral alfa/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Proteína X Associada a bcl-2/metabolismo
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