RESUMO
Two new labdane-type diterpenes (adenanthic acids A and B; 1 and 2, resp.) and three new labdane diterpene glycosides (adenanthosides A-C; 3-5, resp.) were isolated from the roots of Isodon adenantha, together with 23 known constituents including seven diterpenoids (6-12), eight triterpenoids (13-20), one lignan glycoside (21), six steroids (22-27), and one ceramide (28). Their structures were elucidated by spectroscopic methods including extensive 2D-NMR techniques. Cytotoxicity and antibacterial activities of the samples were measured by the MTT method and the filter paper disc agar diffusion method. But none of them showed significant activities.
Assuntos
Diterpenos/química , Glicosídeos/química , Isodon/química , Raízes de Plantas/química , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Humanos , Espectroscopia de Ressonância MagnéticaRESUMO
Five new terpenoids including 2 labdane diterpenoids (1 and 2), 2 ent-kaurane diterpenoids (3 and 4), and a new oleanane triterpenoid (5), along with 13 known compounds (6-18), were isolated from the ethyl acetate fraction of 70% acetone extract of the rhizomes of Isodonadenantha. Their structures were elucidated based on the analyses of spectroscopic data and comparison of their physicochemical properties. The structure of 1 was further confirmed by single-crystal X-ray diffraction. Cytotoxicity and antibacterial activities of the samples were measured by MTT method and the filter paper disk agar diffusion method. But none of them showed significant activities.
Assuntos
Isodon/química , Terpenos/isolamento & purificação , Diterpenos do Tipo Caurano/química , Diterpenos do Tipo Caurano/isolamento & purificação , Humanos , Estrutura Molecular , Ácido Oleanólico/química , Ácido Oleanólico/isolamento & purificação , Rizoma/química , Terpenos/químicaRESUMO
To analyze components of Citrus reticulata and salt-processed C. reticulata by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and compared the changes in components before and after being processed with salt. Principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA) were adopted to analyze the difference in fingerprint between crude and processed C. reticulata, showing increased content of eriocitrin, limonin, nomilin and obacunone increase in salt-processed C. reticulata. Potential chemical markers were identified as limonin, obacunone and nomilin, which could be used for distinguishing index components of crude and processed C. reticulata.
Assuntos
Citrus/química , Sais/química , Benzoxepinas/química , Cromatografia Líquida de Alta Pressão/métodos , Análise Discriminante , Limoninas/química , Espectrometria de Massas/métodos , Análise de Componente Principal/métodosRESUMO
The processing technology employed in traditional Chinese medicine (TCM) is significant and distinct. Meanwhile, the processed Coptis chinensis Franch. are significant in clinic based on clinical practice and literature. The current study used ultraperformance liquid chromatography method (UPLC) coupled with quadrupole time of flight mass spectrometry (qTOF/MS) and Marklynx software to analyze the chemical profiles of crude and processed C. chinensis Franch. 13 compounds in these samples are identified, including 3 compounds that are detected in C. chinensis Franch. for the first time. Moreover, the results of the experiment show significant chemical differences between crude and processed C. chinensis Franch. with principal component analysis (PCA). The obvious separation in PCA confirms the traditional processing theory in TCM.
