1.
Methods Mol Biol
; 1787: 101-113, 2018.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29736713
RESUMO
Neurogenesis phenotypic screen of small-molecule library enables the discovery of small-molecule inducers, and identification of associated biological targets and pathways that control neuronal formation from neural progenitor cells (NPCs). Here, we describe protocols for preparing mouse embryonic NPCs, setting up a high-content imaging assay that quantifies the production of Tuj1-labeled neurons, and analysis of high-throughput screens.