RESUMO
Ports have been built or expanded in a number of countries to cater to increasing maritime trade in the 21st century. Port expansion is associated with economic and environmental impacts on the local and regional scales, and these impacts can be studied using remote sensing. The present study presents new results from multi-source remote sensing monitoring of the Ajmr Port expansion. An analysis of land use and vegetation coverage at the port is used to monitor the impact of port construction on the local ecology, while changes in roads, buildings, and lights are used to monitor the economic impact. The results show that: (1) After nine years of expansion, the port area has gradually expanded from the central to the southern coastal area, with an increase of 21.68 hectares during the expansion period. After the expansion, the area of builidings and construction in the study area increased significantly, while the area of water and green areas decreased significantly, indicating that the port construction changed the land use structure of the area. (2) From the perspective of vegetation coverage, the vegetation coverage within 5 km from the port is in good condition. After 9 years, the vegetation coverage in the region between 0.6 and 1 increased from 43.71% to 44.25%, reflecting the higher overall greening level in the region. (3) By analyzing the increase in roads and buildings, it can be seen that the port's comprehensive transportation capacity has improved, the population of the region has increased significantly. As the scale of construction has been continuously expanded , the prosperity as increased. (4) By analyzing the changes in the light index, the light data from the northeast to the southwest in the region is very obvious, and it is clearly located along the coast, indicating that the economic development of the coastal zone is faster than other regions, and the coastal region has promoted the development of the inland region.
RESUMO
The aim of this study was to determine the role of ALDH2 in the injury of liver from brain-dead donors. Using brain-dead rabbit model and hypoxia model, levels of ALDH2 and apoptosis in tissues and cell lines were determined by Western blot, flow cytometry (FCM), and transferase (TdT)-mediated biotin-16-dUTP nick-end labeling (TUNEL) assays. After the expression of ALDH2 during hypoxia had been inhibited or activated, the accumulations of 4-hydroxynonenal (4-HNE) and molecules involved in mitogen-activated protein kinase (MAPK) signaling pathway were analyzed using ELISA kit and Western blot. The low expression of phosphorylated ALDH2 in liver was time-dependent in the brain-dead rabbit model. Immunohistochemistry showed ALDH2 was primarily located in endothelial, and the rates of cell apoptosis in the donation after brain-death (DBD) rabbit groups significantly increased with time. Following the treatment of inhibitor of ALDH2, daidzein, in combination with hypoxia for 8 h, the apoptosis rate and the levels of 4-HNE, P-JNK, and cleaved caspase-3 significantly increased in contrast to that in hypoxic HUVECs; however, they all decreased after treatment with Alda-1 and hypoxia compared with that in hypoxic HUVECs (P < 0.05). Instead, the levels of P-P38, P-ERK, P-JNK, and cleaved caspase-3 decreased and the ratio of bcl-2/bax increased with ad-ALDH2 (10(6) pfu/ml) in combination with hypoxia for 8 h, which significantly alleviated in contrast to that in hypoxic HUVECs. We found low expression of ALDH2 and high rates of apoptosis in the livers of brain-dead donor rabbits. Furthermore, decreased ALDH2 led to apoptosis in HUVECs through MAPK pathway.
