Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 33
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Heliyon ; 9(9): e20160, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37809983

RESUMO

Acinic cell carcinoma (ACCA), a type of malignant epithelial neoplasm, tends to occur in the parotid gland, and is occasionally found within the breast. Published literature regarding primary ACCA of the breast is scarce, and the number of reports may be fewer than 100. At present, full clinical details have not been published. As an extremely rare disorder, ACCA cannot be definitively diagnosed depending on microscopic structure alone and often requires the assistance of immunohistochemistry. Currently, universal therapies are not available. Here, we present a 47-year-old patient with a history of a palpable mass in the outer upper quadrant of the left breast for more than 2 years, which had obviously increased in size in the last half year. This patient was definitively diagnosed with primary ACCA of the breast. Neoadjuvant chemotherapy was performed preoperatively, and drug sensitivity tests based on primary tumor cells were conducted after surgery and successfully screened chemotherapy schemes for the patient's greater benefit. The whole treatment course followed the guidelines for invasive breast cancer. The patient was free of symptoms for 14 months after surgery. Long-term follow-up is in progress. Altogether, to further broaden the understanding of primary ACCA of the breast, we detail the diagnosis and treatment of one patient and review the relevant literature.

2.
Front Cell Dev Biol ; 10: 862180, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35465321

RESUMO

Recurrent spontaneous abortion (RSA) is defined as the loss of two or more consecutive intrauterine pregnancies that are clinically established early in pregnancy. To date, the etiology and underlying mechanisms of RSA remain unclear. It is widely thought that the impairment of decidualization is inclined to induce subsequent pregnancy failure and leads to the dysregulation of extra-villous trophoblast invasion and proliferation through maternal-fetal cross talk. However, the mechanism of decidualization in RSA has yet to be understood. In our study, we demonstrate that decidual samples from RSA patients have significantly higher insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) and lower TGF-ß1 levels compared to healthy controls. In addition, the overexpression of IGF2BP3 in human endometrial stromal cells (hESCs) can lead to the impairment of decidualization in vitro-induced model and the abnormal cell cycle regulation. Furthermore, TGF-ß1 and MMP9 levels were greatly increased after decidualization, whereas IGF2BP3 overexpression inhibited endometrial mesenchymal decidualization by downregulating TGF-ß1, impeding maternal-fetal interface cytokine cross talk, and limiting the ability of trophoblast invasion. In conclusion, our investigation first demonstrates that abnormal elevation of IGF2BP3 in the pregnant endometrium leads to the impairment of decidualization and abnormal trophoblast invasion, thereby predisposing individuals to RSA.

3.
Chinese Journal of Hepatology ; (12): 113-116, 2022.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-935920

RESUMO

Clinically, patients with tuberculosis (TB) combined with hepatitis C virus (HCV) infection often require simultaneous treatment. Consequently, when anti-HCV and TB drugs are used in combination drug-drug interactions (DDIs), anti-TB drug-induced hepatotoxicity, and liver disease states need to be considered. This paper focuses on discussing the metabolic mechanisms of commonly used anti-TB and HCV drugs and the selection options of combined drugs, so as to provide rational drug use for TB patients combined with HCV infection.


Assuntos
Humanos , Doença Hepática Induzida por Substâncias e Drogas , Coinfecção/tratamento farmacológico , Hepacivirus , Hepatite C/tratamento farmacológico , Preparações Farmacêuticas , Tuberculose/tratamento farmacológico
4.
J Oncol ; 2021: 5523749, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34484333

RESUMO

BACKGROUND: Epithelial ovarian cancer (EOC) is an extremely lethal gynecological malignancy and has the potential to benefit from the immune checkpoint blockade (ICB) therapy, whose efficacy highly depends on the complex tumor microenvironment (TME). METHOD AND RESULT: We comprehensively analyze the landscape of TME and its prognostic value through immune infiltration analysis, somatic mutation analysis, and survival analysis. The results showed that high infiltration of immune cells predicts favorable clinical outcomes in EOC. Then, the detailed TME landscape of the EOC had been investigated through "xCell" algorithm, Gene set variation analysis (GSVA), cytokines expression analysis, and correlation analysis. It is observed that EOC patients with high infiltrating immune cells have an antitumor phenotype and are highly correlated with immune checkpoints. We further found that dendritic cells (DCs) may play a dominant role in promoting the infiltration of immune cells into TME and forming an antitumor immune phenotype. Finally, we conducted machine-learning Lasso regression, support vector machines (SVMs), and random forest, identifying six DC-related prognostic genes (CXCL9, VSIG4, ALOX5AP, TGFBI, UBD, and CXCL11). And DC-related risk stratify model had been well established and validated. CONCLUSION: High infiltration of immune cells predicted a better outcome and an antitumor phenotype in EOC, and the DCs might play a dominant role in the initiation of antitumor immune cells. The well-established risk model can be used for prognostic prediction in EOC.

5.
Brain Res Bull ; 154: 106-115, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31722250

RESUMO

Single-nucleotide polymorphism (SNP) and Alternative splicing (AS) were found to be implicated in certain diseases, nevertheless, the contributions of mRNA SNPs and AS to pathogenesis in developing rat brains with hypoxic-ischemic encephalopathy (HIE) remained largely vague. Additionally, the disease associated with Tacr3 was normosmic congenital hypogonadotropic hypogonadism, while the relationship between HIE and Tacr3 remained largely elusive. The current study was designed to investigate the differentially expressed mRNAs and related SNPs as well as AS in neonatal rats subjected to HIE to identify if the exhibition of AS was associated with SNPs under pathological condition. Firstly, we used postnatal day 7 Sprague-Dawley rats to construct neonatal HIE model, and analyzed the expression profiles of SNP mRNA in hypoxic-ischemic (HI) and sham brains by using RNA sequencing. Then four genes, including Mdfic, Lpp, Bag3 and Tacr3, connecting with HIE and exhibiting SNPs and AS were identified by bioinformatics analysis. Moreover, combined with exonic splicing enhancer (ESE) and alternative splice site predictor (ASSP) analysis, we found that Tacr3 is associated specifically with HIE through 258547789 G > A SNP in inside the Alt First Exon and 258548573 G > A SNP in outside the Alt First Exon. Taken together, our study provides new evidence to understand the role of Tacr3 in HIE and it is possibly a potential target for the treatment of HIE in future clinic trial.


Assuntos
Hipóxia-Isquemia Encefálica , Receptores de Taquicininas , Animais , Humanos , Masculino , Ratos , Processamento Alternativo/genética , Animais Recém-Nascidos , Encéfalo/metabolismo , Modelos Animais de Doenças , Hipóxia-Isquemia Encefálica/genética , Hipóxia-Isquemia Encefálica/metabolismo , Neurônios/metabolismo , Polimorfismo de Nucleotídeo Único/genética , Ratos Sprague-Dawley , Receptores da Neurocinina-3/genética , Receptores da Neurocinina-3/metabolismo , Receptores de Taquicininas/genética , Receptores de Taquicininas/metabolismo
6.
Journal of Practical Radiology ; (12): 941-943, 2018.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-696943

RESUMO

Objective To investigate the short-term clinical effect of CT guided 125Ⅰ radioactive particle therapy in superficial malignant tumor. Methods The clinic data of 28 patients with metastatic superficial malignant tumor in our hospital were analyzed retrospectively.All patients were treated with CT guided 125Ⅰ radioactive particle therapy.The short-term effects,1 year survival rate and 1 year progression free survival rate of the patients were compared.Results Objective remission rate(ORR)and disease control rate(DCR)after 6 months were 92.86% and 100.00%.1 year overall survival and 1 year progression free survival were 96.43%(27/28)and 82.14%(23/28), respectively.The median overall survival and median progression free survival were 26.978 months (95%CI:22.558-31.399)and 16.932 months (95 % CI:14 .471-19.393).There were 27 cases of 0-Ⅱ degree adverse reactions,1 case of grade Ⅲ adverse reactions and no grade Ⅳ adverse reactions.No signs of 125Ⅰ radioactive particle translocation,vascular embolism and vascular rupture were found. Conclusion 125Ⅰ radioactive particle treatment of superficial malignant tumor has a definite short-term curative effect,with overall survival and progression free survival longer and higher safety,which can be considered in clinical application.

7.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-692292

RESUMO

The feature of gasoline Raman spectra which were used to study the quantitative analysis of the research octane number (RON) were extracted for the first time using backward interval partial least squares (BiPLS). In the experiment, the sample set partitioning based on joint x-y distances (SPXY) method was used to divide the training set, the cross validation set and the test set. And the robust regression algorithm was used to remove the abnormal sample. The partial least squares model was established using feature selected by the BiPLS algorithm. Compared with the model without feature selection, it was shown that the backward interval partial least squares algorithm could reduce the input dimension by 50.00%, and the root mean square error of cross validation(RMSECV) by 18.92% and the root mean square error of prediction (RMSEP) by 13. 86%. The backward interval partial least squares algorithm can effectively extract the feature from gasoline Raman spectrum,reduce the model complexity, and improve the prediction accuracy of the model,and has great application prospect in the quantitative analysis of research octane number.

8.
Elife ; 62017 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-28134617

RESUMO

A large number of drugs can induce prolongation of cardiac repolarization and life-threatening cardiac arrhythmias. The prediction of this side effect is however challenging as it usually develops in some genetically predisposed individuals with normal cardiac repolarization at baseline. Here, we describe a platform based on a genetically diverse panel of induced pluripotent stem cells (iPSCs) that reproduces susceptibility to develop a cardiotoxic drug response. We generated iPSC-derived cardiomyocytes from patients presenting in vivo with extremely low or high changes in cardiac repolarization in response to a pharmacological challenge with sotalol. In vitro, the responses to sotalol were highly variable but strongly correlated to the inter-individual differences observed in vivo. Transcriptomic profiling identified dysregulation of genes (DLG2, KCNE4, PTRF, HTR2C, CAMKV) involved in downstream regulation of cardiac repolarization machinery as underlying high sensitivity to sotalol. Our findings offer novel insights for the development of iPSC-based screening assays for testing individual drug reactions.


Assuntos
Arritmias Cardíacas/induzido quimicamente , Cardiotoxinas/metabolismo , Células-Tronco Pluripotentes Induzidas/efeitos dos fármacos , Células-Tronco Pluripotentes Induzidas/fisiologia , Programas de Rastreamento/métodos , Antiarrítmicos/metabolismo , Perfilação da Expressão Gênica , Humanos , Modelos Biológicos , Sujeitos da Pesquisa
9.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 29(3): 329-333, 2017 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-29469524

RESUMO

OBJECTIVE: To identify the genotype of Toxoplasma gondii isolated strains from a congenital teras (KS strain) and an HIV-Toxoplasma co-infected patient in Jiangsu Province. METHODS: T. gondii DNA of tachyzoites of a isolate from a congenital teras (KS strain) and blood DNA of an HIV-Toxoplasma co-infected patient in Jiangsu Province were extracted, and 11 loci were identified for the genotype by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). RESULTS: The complete bands were obtained from the congenital teras (KS strain) and HIV-Toxoplasma co-infected patient in Jiangsu Province, and identified as T. gondii gene type I. CONCLUSIONS: T. gondii gene type I may be the dominant genotype strain of T. gondii among the women who have the abnormal pregnant outcomes and HIV-Toxoplasma co-infected patients in Jiangsu Province.


Assuntos
Genótipo , Infecções por HIV/parasitologia , Toxoplasma/genética , Coinfecção/parasitologia , Coinfecção/virologia , DNA de Protozoário/isolamento & purificação , Humanos , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição
10.
Artigo em Chinês | MEDLINE | ID: mdl-30133238

RESUMO

Objective: To perform a proteomics analysis for metacercariae, juvenile and adult worms of Paragonimus skrjabini. Methods: Crabs were collected in P. skrjabini endemic areas of Kaiyang and Bijie in Guizhou Province. Metacercariae were isolated, placed in PBS, and were used to infect three SD rats(10 metacercariae/rat) by intragastric administration and infect three male dogs(20 metacercariae/dog) through feeding. The rats were sacrificed at 1 month after infection to obtain juvenile worms. The dogs were sacrificed at 3 months after infection to obtain adult worms. The metacercariae, juvenile and adult worms were lysed, and total protein was extracted by ultrasonication. The total protein content was determined by Bradford method and separated by SDS-PAGE and two dimensional gel electrophoresis. Images of two dimensional gel electrophoresis were analyzed using the PDquest 8.0 software. The dots with difference were digested and analyzed with mass spectrometry. Finally, online searches in NCBI and local databases were performed. Results: Results of SDS-PAGE showed that the total protein of metacercariae, juvenile and adult worms was concentrated within Mr of 25 000-116 000. Fifty-one protein dots with difference were found by two dimensional gel electrophoresis, comprising of 20 dots for metacercariae, 25 for juvenile worms and 6 for adult worms. Thirty-six peptide sequences of metacercariae and juvenile worms were analyzed. They were basically determined to be Achrornobacter lyticus protease Ⅰ(a lysine-specific serine protease), ascorbate reductase protein, glutathione s-transferase DHAR2 analogue, heat shock proteins Hsp82 and Hsp96-ß, actin, cystatin, etc., by online searches, and cysteine, actin and heat shock protein by local searches in the diginea database(downloaded to a local computer from NCBI). Mass spectrometry was not performed for adult worms, as the variation of grayscale value between their spots was far less than those for metacercariae and juvenile worms. Conclusion: One difference is that the metacercariae of P. skrjabini have actin, while the juvenile worms have detoxification proteins and stress proteins. But they both have hydrolases and cysteine enzymes.


Assuntos
Paragonimíase , Paragonimus , Animais , Braquiúros , Masculino , Proteômica , Ratos , Ratos Sprague-Dawley
11.
Journal of Xinxiang Medical College ; (12): 1015-1020, 2017.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-669359

RESUMO

Objective To investigate the correlation between the gene polymorphism of homocysteine metabolic enzyme cystathionine β-synthase(CBS) 844ins68,N5,10-methylenetetrahydrofolate reductase(MTHFR) C677T and chronic pulmonary heart disease(CPHD).Methods A total of 230 patients with CPHD in observation group were selected from January 2014 to November 2016 in the Second People's Hospital of Xinxiang City,and 235 healthy subjects in healthy control group were selected at the same time.The lung function test was performed with lung function instrument,and the percentage of the forced expiratory volume in one second to predicted value(FEV1% pred) and the forced expiratory volume in one second to forced vital capacity(FEV1%) value were recorded in the two groups.The fasting ulnar venous blood was collected from the patients in the observation group on the next morning after hospitalization and the subjects in the control group on the morning of health examination.The levels of plasma homocysteine (Hcy),fasting blood glucose (FBG),triacylglycerol (TG),total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were detected.The DNA was extracted from the whole blood cells.The CBS 844ins68 polymorphism was detected by polymerase chain reaction genotyping.The MTHFR C677T polymorphism was detected by restriction fragment length polymorphism polynerase chain reaction.Results There was no significant difference in the FBG level between the two groups (P > 0.05).The levels of Hcy,TG,TC and LDL-C in the observation group were significantly higher than those in the healthy control group (P < 0.05),and the FEV1 and FEV1% pred were significantly lower than those in the healthy control group (P < 0.05).There were two genotypes of CBS 844ins68 in the two groups.The genotype frequencie of DD and DI in the observation group was 91.74% and 8.26%,and the allele frequency of D and I was 95.87% and 4.13% respectively.The genotype frequency of DD and DI in the healthy control group was 94.04% and 5.96%,and the allele frequency of D and I was 97.02% and 2.98% respectively.There was no significant difference in genotype and allele frequency distribution between the two groups (x2 =0.935,0.901;P > 0.05).Three genotypes of MTHFRC677T were detected after enzyme digestion in the two groups.The genotype frequency of CC,CT and TT in the healthy control group was 27.66%,48.94% and 23.40%;and the allele frequency of C and T was 52.13% and 47.87% respectively.The frequency of TT genotype and T allele in the observation group was significantly higher than that in the healthy control group (x2 =7.730,7.326;P < 0.05).Conclusions Hcy level increasing may be a risk factor for CPHD.The polymorphisms of CBS 844ins68 gene may be unrelated to the occurrence of CPHD.The polymorphism of the MTHFR C677T gene may contribute to CPHD by affecting Hcy level.The T allele of MTHFR C677T may be a risk factor for CPHD,and the MTHFRC677T gene may be a genetic predisposition to CPHD.

12.
Journal of Chinese Physician ; (12): 52-54, 2016.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-493665

RESUMO

Objective To summarize and analyze the adverse reactions caused by cancer chemo-therapy drugs in clinical practice and the specific influencing factors,so as to make more scientific and ef-fective chemotherapy for the treatment of cancer patients.Methods 80 cases of cancer patients treated in our hospital from December to October 2013 in were selected as the research objects.Results Adverse effects of cancer chemotherapy drugs emerged strictly speaking is inevitable,the patient should be the high-est incidence of bad hair in the digestive system,boil down to:nausea,loss of appetite,alopecia and diar-rhea.Conclusions Fully grasp the application of chemotherapeutic drugs adverse reaction and its influen-cing factors,and for making treatment scheme,for improving the treatment effect has important clinical sig-nificance.

13.
Cancer Research and Clinic ; (6): 646-648, 2015.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-482589

RESUMO

Although the treatment has made great progress in breast cancer, there are still many problems. For example, primary or secondary on chemotherapy drug resistance and the limitation of treatment in triple-negative breast cancer. So, targeted therapy has become a new strategy to improve this situation. Previous studies showed that the occurrence of breast cancer may be related to the activation of PI3K/Akt/mTOR signaling pathways, and mTOR is an index to judge the prognosis. Parts of breast cancers can obtain clinical benefit with mTOR inhibitors, and it might be a potential treatment target in breast cancer.

14.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-290177

RESUMO

Although neurophysiological and psychophysical proof of osseoperception is accumulating, histomorphometric evidence for the neural mechanisms of functional compensation following immediate and delayed implant loading is still lacking. For this randomized split-mouth study, six mongrel dogs randomly received one of four treatment protocols at 36 implant-recipient sites over 16 weeks (third maxillary incisor, third and fourth mandibular premolar): immediate implant placement and immediate loading (IIP+IL); delayed implant placement and delayed loading (DIP+DL); delayed implant placement and immediate loading (DIP+IL); and natural extraction socket healing (control). Histomorphometry was performed in the peri-implant bone and soft tissues within 300 µm around the implants. Immunocytochemistry and transmission electron microscopy were used to confirm the presence of neural structures and to reveal their ultrastructural characteristics, respectively. Myelinated nerve fibres densely populated the peri-implant crestal gingival and apical regions, although they were also identified in the woven bone and in the osteons near the implant threads. Compared with the control group in the mandible, the group that received IIP+IL showed a higher innervation (in N⋅mm⁻², 5.94 ± 1.12 vs. 3.15 ± 0.63, P<0.001) and smaller fibre diameter (in µm, 1.37 ± 0.05 vs. 1.64 ± 0.13, P=0.016), smaller axon diameter (in µm, 0.89 ± 0.05 vs. 1.24 ± 0.10, P=0.009) and g-ratio (0.64 ± 0.04 vs. 0.76 ± 0.05, P<0.001) in the middle region around the implants. Compared with DIP+IL in the mandible, IIP+IL had a higher nerve density (in N⋅mm⁻², 13.23 ± 2.54 vs. 9.64 ± 1.86, P=0.027), greater fibre diameter (in µm, 1.32 ± 0.02 vs. 1.20 ± 0.04, P=0.021), greater axon diameter (in µm, 0.92 ± 0.01 vs. 0.89 ± 0.03, P=0.035) and lower g-ratio (0.69 ± 0.01 vs. 0.74 ± 0.01, P=0.033) in the apical region around the implants. It may be assumed that the treatment protocol with IIP+IL is the preferred method to allow optimized peri-implant re-innervation, but further functional measurements are still required.


Assuntos
Animais , Cães , Implantes Dentários , Microscopia Eletrônica de Transmissão , Fibras Nervosas , Projetos Piloto
15.
Chinese Journal of Epidemiology ; (12): 1179-1182, 2013.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-321696

RESUMO

<p><b>OBJECTIVE</b>To understand the infection status and epidemiological features of HBV in permanent residents of Shenzhen city.</p><p><b>METHODS</b>A multi-stage stratified random sampling method was performed for questionnaire survey to permanently-registered residents of 1-59 years old in Luohu and Baoan district of Shenzhen in 2010, and blood samples of the subjects were collected. Hepatitis B virus-related surface antigen (HBsAg) and hepatitis B virus surface antibody (anti-HBs) were detected with ELISA.</p><p><b>RESULTS</b>The total 3771 studied population showed 252 HBsAg positive and 2712 anti-HBs positive residents with the standardization prevalence as 9.73% and 72.83% , respectively. The difference of the prevalence of HBsAg and anti-HBs between males and females were not statistically significant (P > 0.05). The prevalence of HBsAg was reduced with increasing age. The differences of the prevalence of HBsAg between Shenzhen permanent registered and non-permanent registered population were not significant, but the prevalence of anti-HBs in Permanent registered residents (78.32%) was higher than in non-permanent (66.03%, χ(2) = 41.613, P < 0.001). The prevalence of HBsAg was significantly different in various occupational and educational levels. Peasants had the highest prevalence (24.13%) and medical workers had the highest prevalence of anti-HBs (89.10% ). People with junior high school education had the highest prevalence of HBsAg (12.76%) and the lowest of anti-HBs (62.45%). Population with high-level education had the highest prevalence of anti-HBs(81.00% average). The prevalence of HBsAg was over 10% in people who were born in Shenzhen and Guangdong province, and the anti-HBs was the highest in Shenzhen population with the prevalence as 74.48% and 76.47% , respectively.</p><p><b>CONCLUSION</b>In the Shenzhen resident population, the overall prevalence of HBV was lower than the average level of Guangdong province, but higher than the national wide.</p>


Assuntos
Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , China , Epidemiologia , Hepatite B , Sangue , Epidemiologia , Estudos Soroepidemiológicos , Inquéritos e Questionários
16.
Chinese Journal of Cardiology ; (12): 28-32, 2013.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-292031

RESUMO

<p><b>OBJECTIVE</b>Regional left ventricular (LV) function could be detected by measuring peak-systolic strain by speckle tracking imaging (STI). We evaluated the value of STI combined with adenosine stress echocardiography on assessing myocardial viability in patients with myocardial infarction (MI).</p><p><b>METHODS</b>Two dimensional echocardiography was performed at rest and after adenosine stress echocardiography (infused at 140 µg×kg(-1)×min(-1) over a period of 6 min) in 39 stable patients with previous MI. Peak-systolic (Speak-sys) circumferential strain, radial strain and longitudinal strain were assessed by STI. Radionuclide myocardial perfusion/metabolic imaging served as the "gold standard" to detection of myocardial viability.</p><p><b>RESULTS</b>(1) There were 215 viable and 153 non-viable regions among 368 abnormal motion segments out of 624 segments in 39 MI patients according to radionuclide imaging results. (2) Speak-sys was similar between viable and nonviable myocardium at rest (all P > 0.05). After adenosine infusion, radial Speak-sys [(37.98 ± 5.45)% vs. (30.22 ± 5.47)%], longitudinal Speak-sys [(-23.71 ± 4.53)% vs. (-17.52 ± 4.34)%] increased significantly (P < 0.05)in viable segments compared to baseline levels and were significantly higher than in nonviable segments radial Speak-sys [(37.98 ± 5.45)% vs. (30.12 ± 5.37)%] and longitudinal Speak-sys [(-23.71 ± 4.53)% vs. (-16.95 ± 4.62)%] (P < 0.05), while remained unchanged in nonviable segments before and after adenosine infusion. Circumferential Speak-sys was similar before and after adenosine infusion in both viable and nonviable segments (all P > 0.05). (3) Delta radial strain change > 9.8% has a sensitivity of 82.3% and a specificity of 81.1% whereas a delta change of longitudinal strain > 16.5% has a sensitivity of 83.5% and a specificity of 92.3% for detecting viable segments.</p><p><b>CONCLUSIONS</b>Speckle tracking imaging combined with adenosine stress echocardiography could serve as a new and reliable method of assessing myocardial viability.</p>


Assuntos
Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sobrevivência Celular , Ecocardiografia , Infarto do Miocárdio , Diagnóstico por Imagem , Miocárdio , Biologia Celular , Cintilografia
17.
Chinese Journal of Cardiology ; (12): 829-833, 2010.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-244136

RESUMO

<p><b>OBJECTIVE</b>to explore the feasibility of evaluating viable myocardium with two-dimensional strain imaging combined with adenosine stress echocardiography.</p><p><b>METHODS</b>acute myocardial infarction and reperfusion model was made by ligating anterior descending coronary artery for 90 minutes followed by 120-minute reperfusion in 15 healthy mongrel dogs. Images were acquired at baseline and after reperfusion. Adenosine was then infused and image acquisition repeated. Regional peak-systolic strain in radial, circumferential and longitudinal motion on anterior wall and anterior septum were measured. TTC staining served as a "gold standard" to define viable and nonviable myocardium. The ratio of infarct area (S(N)) to total area (S) was calculated and viable myocardium was defined with S(N)/S ≤ 50%.</p><p><b>RESULTS</b>at baseline, RS(peak sys), CS(peak sys) and LS(peak sys) were similar between viable (n = 37) and nonviable myocardial segments (n = 53) and significantly decreased after reperfusion in both viable and nonviable myocardial segments. Compared with values obtained after reperfusion, LS(peak sys) and RS(peak sys) remained unchanged in nonviable myocardial segments and significantly increased in viable myocardial segments after adenosine (P < 0.05). Post adenosine RS(peak sys) was negatively correlated with S(N)/S and CS(peak sys) and LS(peak sys) were positively correlated with S(N)/S. With ΔRS(peak-sys) (before and after adenosine) ≥ 13.5%, the sensitivity was 83.8% and specificity was 83.0% for distinguishing viable from nonviable myocardial segment. With ΔLS(peak sys) ≥ 11% as cutoff value, the sensitivity was 78.4% and specificity was 88.7% for distinguishing viable from nonviable myocardial segment. Combining ΔRS(peak sys) and ΔLS(peak sys), the sensitivity and specificity for distinguishing viable from nonviable myocardial segment were 91.9% and 79.2%, respectively.</p><p><b>CONCLUSIONS</b>two-dimensional strain imaging combined with adenosine stress echocardiography could quantitatively identify viable and nonviable myocardium.</p>


Assuntos
Animais , Cães , Modelos Animais de Doenças , Ecocardiografia sob Estresse , Métodos , Infarto do Miocárdio , Diagnóstico por Imagem , Traumatismo por Reperfusão Miocárdica , Diagnóstico por Imagem , Miocárdio , Patologia , Sobrevivência de Tecidos
18.
Chinese Journal of Hepatology ; (12): 184-188, 2010.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-247561

RESUMO

<p><b>OBJECTIVE</b>To analyze the characteristics of CDR3 of TCRbeta on CD8+ T cells in chronic hepatitis B patients.</p><p><b>METHODS</b>Eight patients with chronic hepatitis B (ALT more than 2 ULN) were enrolled in this study. CD8+ T cells were isolated from peripheral blood. RT-PCR was proformed to amplify the CDR3 of TCRbeta, and the PCR products were sequenced and analyzed.</p><p><b>RESULTS</b>The chronic hepatitis B patients showed obvious clonal expansion of T cell, and three perturbation patterns of T cell expansion were showed in the CDR3 of TCRbeta, including monoclonicity, oligoclonicity and skewed peak patterns. The number of perturbation families of CD8+ subpopulation was significantly higher than that of CD8- subpopulation (10.6+/-4.7 vs. 4.1+/-3.1, t = 6.619, P less than 0.01). In 3 out of 8 patients, the number of perturbation families of CD8+ subpopulation was also higher than that of PBMCs without depleting CD8+ subpopulation.</p><p><b>CONCLUSIONS</b>The characteristics of CDR3 of TCRbeta may help to understand the inflammatory response in CHB patients.</p>


Assuntos
Adulto , Humanos , Masculino , Adulto Jovem , Linfócitos T CD8-Positivos , Alergia e Imunologia , Regiões Determinantes de Complementaridade , Genética , Genes Codificadores da Cadeia beta de Receptores de Linfócitos T , Hepatite B Crônica , Sangue , Alergia e Imunologia , Receptores de Antígenos de Linfócitos T , Alergia e Imunologia
19.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-321001

RESUMO

Objective To study the infection status and the molecular characteristics of Vibrio parahaemolyticus isolated from diarrheal patients in Shenzhen, in 2007 to 2008 and to provide evidence for the prevention and control of diarrheal diseases caused by Vibrio parahaemolyticus. Methods More than 80 fecal specimens from four sentinel surveillance hospitals were collected and cultured each month. A total of 361 isolates of Vibrio parahaemolyticus were sero-typed and examined by real-time PCR for the presence of two major virulence genes, tdh and trh. Of 361 strains, 60 O3: K6 strains isolated from six suspected outbreaks in August, 2007 and in September, 2008 were typed by pulsed-field gel electrophoresis (PFGE). Results 4384 stool samples were detected in four sentinel surveillance hospitals and with 361 Vibrio parahaemolyticus strains isolated that belonged to 28 serotypes. Serotype O3:K6, O4:K8 and O1:KUT accounted for 67.90%, 7.50% and 6.10%, respectively. Of 361 strains, 337 strains belonged to tdh + trh- , 11 strains were tdh-trh- and 13 strains were tdh + trh +. The most prevalent serotype which caused diarrheal diseases was tdh + trh-in Shenzhen. The 60 isolates were discriminated into twenty different PFGE patterns, which belonged to three clones. Among the 60 isolates, most of the PFGE patterns of isolates from the suspected outbreak locations were identical and some strains isolated from different year were different. Conclusion Vibrio parahaemolyticus isolates in Shenzhen were dominated by O3:K6 strains. Most of these isolates carried tdh gene and few carried trh gene. Meanwhile, the identical patterns of isolates from 6 suspected outbreaks locations demonstrated that Vibrio parahaemolyticus outbreaks occurred in July 2007 and in September 2008 in Shenzhen. However, the dominated strains' PFGE patterns were different each year, indicating that the sources of Vibrio parahaemolyticus had a multiplex nature and the multiplex sources such as water, sea food and pickled products should be integrated monitored. Laboratory based surveillance of diarrheal diseases could contribute in establishing early warning system for the better prevention and control of diarrheal diseases.

20.
Chinese Journal of Endemiology ; (6): 548-550, 2009.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-642215

RESUMO

Objective To understand current prevalence of fluorosis, of drinking tea type in Pengyang County of Ningxia to offer scientific basis for preventing and curing, monitoring, health education. Methods Four towns were chosen as investigating spots in Pengyang County of Ningxia in 2007, from each 50 children aged 8-12 year old and 50 adults who either had tea or did not were investigated. Dean method was used to measure dental fluorosis of children and adult; meanwhile fluoride content in water, urine and drinking tea was tested using the fluoride ionic electrode method and that in brick tea using water immersion electrode method. χ2 testing, analysis of variance, u test, q test were performed. Results In adult drink tea group, control group, and children, the detaction rate of dental fluorosis were respectively 24.75%(99/400),9.5%(38/400)and 15.96%(71/445), obviously higher in drink tea group than the control group(χ2= 32.73, P<0.05). Urine average concentration of fluoride was (3.38±2.37), (2.09±1.33 ). (1.41±0.67)mg/L, obviously higher in drink tea group than the control group(u= 9.49, P<0.05). In 4 investigation spots tea fluoride averaged at (3.63±2.05)mg/L, fluoride content in drinking water was averagely (1.01±0.49) mg/L, with that of Wangwa Town [(1.31±0.33)mg/L] exceeding the national standard for drinking water (1.0 mg/L), where dental fluorosis rate was higher and fluoride content was higher than that in 3 other investigation spots (P<0.05). Conclusions Residents in Pengyang County in Ningxia who drink brick tea can cause accumulation of fluorine to poison in Ningxia, leading to fluorosis of drinking tea type; Wangwa Town have both drink tea and water fluorine poison.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...