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1.
Environ Pollut ; 358: 124498, 2024 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-38972564

RESUMO

Activated persulfate and hydrothermal treatment (HTT) are often employed to treat waste activated sludge, which can improve the efficiency of subsequent sludge treatment and change the distribution of pollutants in the sludge. However, the impact of sludge solid content and temperature on the occurrence and aging of microplastics (MPs) during HTT remains poorly understood. This study investigated the effects of persulfate-HTT (SPS-HTT) co-treatment on the migration, occurrence, and aging of MPs in sludge with different solid contents (2% and 5% solid content). The results indicated that SPS-HTT co-treatment triggers both the disruption of sludge flocs and the melting deformation of MPs at high temperatures, leading to variations in the increasing trend of MP concentration in the solid-liquid phase at different solid contents. 5% solid content sludge showed a weak release of MPs from the solid phase. The proportion of fiber MPs first increased and then decreased with increasing temperature, while no significant changes were observed in the color and type of MPs. Higher temperature and solid content induced the melting deformation of MPs, exacerbated the aging of polypropylene MPs, and resulted in rough surfaces, higher carbonyl index, and variations in crystallinity. Moreover, the correlation between the carbonyl index and aging indicators increased with increasing solid content. The MP-derived dissolved organic matter under HTT primarily comprised soluble microbial by-products and humic acid-like substances. These findings underscore the significance of sludge solid content in affecting the migration and aging of MPs during HTT, and offer novel insights into the application of HTT to MP management in sludge treatment.

2.
Front Pharmacol ; 15: 1392338, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38966547

RESUMO

Introduction: Alcohol consumption alters the diversity and metabolic activities of gut microbiota, leading to intestinal barrier dysfunction and contributing to the development of alcoholic liver disease (ALD), which is the most prevalent cause of advanced liver diseases. In this study, we investigated the protective effects and action mechanism of an aqueous extraction of Pericarpium citri reticulatae and Amomi fructus (PFE) on alcoholic liver injury. Methods: C57BL/6 mice were used to establish the mouse model of alcoholic liver injury and orally administered 500 and 1,000 mg/kg/d of PFE for 2 weeks. Histopathology, immunohistochemistry, immunofluorescence, Western blotting, qRT-PCR, and 16S rDNA amplicon sequencing were used to analyze the mechanism of action of PFE in the treatment of alcohol-induced liver injury. Results: Treatment with PFE significantly improved alcohol-induced liver injury, as illustrated by the normalization of serum alanine aminotransferase, aspartate aminotransferase, total triglyceride, and cholesterol levels in ALD mice in a dose-dependent manner. Administration of PFE not only maintained the intestinal barrier integrity prominently by upregulating mucous production and tight junction protein expressions but also sensibly reversed the dysregulation of intestinal microecology in alcohol-treated mice. Furthermore, PFE treatment significantly reduced hepatic lipopolysaccharide (LPS) and attenuated oxidative stress as well as inflammation related to the TLR4/NF-κB signaling pathway. The PFE supplementation also significantly promoted the production of short-chain fatty acids (SCFAs) in the ALD mice. Conclusion: Administration of PFE effectively prevents alcohol-induced liver injury and may also regulate the LPS-involved gut-liver axis; this could provide valuable insights for the development of drugs to prevent and treat ALD.

3.
Int J Mol Sci ; 25(12)2024 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-38928262

RESUMO

Cancer is a significant global public health issue with increasing morbidity and mortality rates. To address this challenge, novel drug carriers such as nano-materials, liposomes, hydrogels, fibers, and microspheres have been extensively researched and utilized in oncology. Among them, polymer microspheres are gaining popularity due to their ease of preparation, excellent performance, biocompatibility, and drug-release capabilities. This paper categorizes commonly used materials for polymer microsphere preparation, summarizes various preparation methods (emulsification, phase separation, spray drying, electrospray, microfluidics, and membrane emulsification), and reviews the applications of polymer microspheres in cancer diagnosis, therapy, and postoperative care. The current status and future development directions of polymer microspheres in cancer treatment are analyzed, highlighting their importance and potential for improving patient outcomes.


Assuntos
Microesferas , Neoplasias , Polímeros , Humanos , Neoplasias/diagnóstico , Neoplasias/terapia , Polímeros/química , Portadores de Fármacos/química , Animais , Antineoplásicos/uso terapêutico , Sistemas de Liberação de Medicamentos/métodos
4.
PLoS Biol ; 22(6): e3002666, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38905316

RESUMO

Breast cancer is the most prevalent malignancy and the most significant contributor to mortality in female oncology patients. Potassium Two Pore Domain Channel Subfamily K Member 1 (KCNK1) is differentially expressed in a variety of tumors, but the mechanism of its function in breast cancer is unknown. In this study, we found for the first time that KCNK1 was significantly up-regulated in human breast cancer and was correlated with poor prognosis in breast cancer patients. KCNK1 promoted breast cancer proliferation, invasion, and metastasis in vitro and vivo. Further studies unexpectedly revealed that KCNK1 increased the glycolysis and lactate production in breast cancer cells by binding to and activating lactate dehydrogenase A (LDHA), which promoted histones lysine lactylation to induce the expression of a series of downstream genes and LDHA itself. Notably, increased expression of LDHA served as a vicious positive feedback to reduce tumor cell stiffness and adhesion, which eventually resulted in the proliferation, invasion, and metastasis of breast cancer. In conclusion, our results suggest that KCNK1 may serve as a potential breast cancer biomarker, and deeper insight into the cancer-promoting mechanism of KCNK1 may uncover a novel therapeutic target for breast cancer treatment.


Assuntos
Neoplasias da Mama , Proliferação de Células , Histonas , Animais , Feminino , Humanos , Camundongos , Neoplasias da Mama/patologia , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Glicólise/genética , Histonas/metabolismo , L-Lactato Desidrogenase/metabolismo , L-Lactato Desidrogenase/genética , Lactato Desidrogenase 5/metabolismo , Lactato Desidrogenase 5/genética , Camundongos Endogâmicos BALB C , Camundongos Nus , Invasividade Neoplásica , Metástase Neoplásica , Canais de Potássio de Domínios Poros em Tandem/metabolismo , Canais de Potássio de Domínios Poros em Tandem/genética , Prognóstico , Regulação para Cima/genética
5.
Nat Commun ; 15(1): 4740, 2024 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-38834545

RESUMO

Mitophagy is critical for mitochondrial quality control and function to clear damaged mitochondria. Here, we found that Burkholderia pseudomallei maneuvered host mitophagy for its intracellular survival through the type III secretion system needle tip protein BipD. We identified BipD, interacting with BTB-containing proteins KLHL9 and KLHL13 by binding to the Back and Kelch domains, recruited NEDD8 family RING E3 ligase CUL3 in response to B. pseudomallei infection. Although evidently not involved in regulation of infectious diseases, KLHL9/KLHL13/CUL3 E3 ligase complex was essential for BipD-dependent ubiquitination of mitochondria in mouse macrophages. Mechanistically, we discovered the inner mitochondrial membrane IMMT via host ubiquitome profiling as a substrate of KLHL9/KLHL13/CUL3 complex. Notably, K63-linked ubiquitination of IMMT K211 was required for initiating host mitophagy, thereby reducing mitochondrial ROS production. Here, we show a unique mechanism used by bacterial pathogens that hijacks host mitophagy for their survival.


Assuntos
Proteínas de Bactérias , Burkholderia pseudomallei , Macrófagos , Mitocôndrias , Mitofagia , Burkholderia pseudomallei/metabolismo , Burkholderia pseudomallei/patogenicidade , Burkholderia pseudomallei/fisiologia , Burkholderia pseudomallei/genética , Animais , Camundongos , Mitocôndrias/metabolismo , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/genética , Humanos , Macrófagos/microbiologia , Macrófagos/metabolismo , Ubiquitinação , Melioidose/microbiologia , Melioidose/metabolismo , Interações Hospedeiro-Patógeno , Espécies Reativas de Oxigênio/metabolismo , Sistemas de Secreção Tipo III/metabolismo , Sistemas de Secreção Tipo III/genética , Camundongos Endogâmicos C57BL , Membranas Mitocondriais/metabolismo , Células HEK293 , Células RAW 264.7
6.
J Nat Med ; 2024 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-38775895

RESUMO

The practice of Chinese herbal medicines for the treatment of COVID-19 in China played an essential role for the control of mortality rate and reduction of recovery time. The iridoids is one of the main constituents of many heat-clearing and detoxifying Chinese medicines that were largely planted and frequently used in clinical practice. Twenty-three representative high content iridoids from several staple Chinese medicines were obtained and tested by a SARS-CoV-2 pseudo-virus entry-inhibition assay on HEK-293 T/ACE2 cells, a live HCoV-OC43 virus infection assay on HRT-18 cells, and a SARS-CoV-2 3CL protease inhibitory FRET assay followed by molecular docking simulation. The anti-pulmonary inflammation activities were further evaluated on a TNF-α induced inflammation model in A549 cells and preliminary SARs were concluded. The results showed that specnuezhenide (7), cornuside (12), neonuezhenide (15), and picroside III (21) exhibited promising antiviral activities, and neonuezhenide (15) could inhibit 3CL protease with an IC50 of 14.3 µM. Docking computation showed that compound 15 could bind to 3CL protease through a variety of hydrogen bonding and hydrophobic interactions. In the anti-pulmonary inflammation test, cornuside (12), aucubin (16), monotropein (17), and shanzhiside methyl ester (18) could strongly decrease the content of IL-1ß and IL-8 at 10 µM. Compound 17 could also upregulate the expression of the anti-inflammatory cytokine IL-10 significantly. The iridoids exhibited both anti-coronavirus and anti-pulmonary inflammation activities for their significance of existence in Chinese herbal medicines, which also provided a theoretical basis for their potential utilization in the pharmaceutical and food industries.

8.
J Contam Hydrol ; 264: 104356, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38723447

RESUMO

Karst groundwater plays an irreplaceable role in the formation and development of urban areas, and land-use and land-cover change (LUCC) and the input of pollutants during the urbanization process would pose potential environmental risks to underground rivers. We analysed the relationship between urbanization processes and underground river hydrochemistry over nearly 35 years in Guiyang city, southwest of China, it was found that concentrations of various cations and anions, as well as total dissolved solids (TDS), gradually increased with the urbanization process, with significant fluctuations during the rapid urbanization periods. The Hydrochemical Facies Evolution Diagram (HFED) clearly showed the influence of urbanization on the hydrochemistry of the underground rivers. The ion ratios of γMg2+/γCa2+-γHCO3-, γNa+/γCl-, Ca2+/Mg2+-Ca2+ or Mg2+/Σ cations, HCO3-/SO42--HCO3- or SO42-/Σ anions revealed two distinct phases in the hydrochemical evolution of the underground river system, highly consistent with the urbanization process. Before the rapid urbanization, acid deposition and agricultural activities affected the hydrochemistry, with HCO3-Ca·Mg and HCO3·SO4-Ca·Mg as the dominant types controlled by limestone and dolomite dissolution in water-rock interactions. As acid deposition diminished, the input of SO42- from urban sewage compensated for the reduced impact, but the increased impermeable surfaces reduced the infiltration of atmospheric precipitation, leading to a reduced dissolution of dolomite minerals in water-rock interactions, resulting in a decrease in Mg2+ and a change in the hydrochemical type. The hydrochemical type evolved from a single HCO3·SO4-Ca·Mg type and HCO3-Ca·Mg type to multiple types, such as HCO3·Cl-Ca, HCO3·SO4-Ca, HCO3-Ca, and HCO3·SO4-Ca·Mg, and was highly unstable. With changes in land use, the proportions of various cations and anions in the hydrochemistry changed, especially NH4+, NO3-, SO42-, Na+, and Cl-, which were more sensitive to human activities. This study indicated the impact of urbanization on the hydrochemistry of the underground river system, with the input of SO42- from human activities and the increase in paved surfaces due to urbanization collectively altering the hydrochemical types of the underground river system. The rapid response of karst underground river system hydrochemistry indicates a potential impact on groundwater system by urbanization that should not be ignored.


Assuntos
Monitoramento Ambiental , Água Subterrânea , Rios , Urbanização , Poluentes Químicos da Água , Rios/química , China , Água Subterrânea/química , Água Subterrânea/análise , Poluentes Químicos da Água/análise , Monitoramento Ambiental/métodos
9.
Ecol Evol ; 14(5): e11459, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38774145

RESUMO

Plant invasions severely threaten natural ecosystems, and invasive plants often outcompete native plants across various ecosystems. Arbuscular mycorrhizal (AM) fungi, serving as beneficial microorganisms for host plants, can greatly influence the competitive outcomes of invasive plants against native plants. However, it remains unclear how AM fungi alter the competitive balance between native and invasive species. A competitive experiment was conducted using an invasive Eupatorium adenophorum paired with a native congener Eupatorium lindleyanum. Specifically, both species were inoculated with (M+) or without (M-) the fungus Glomus etunicatum under intraspecific (Intra-) and interspecific (Inter-) competition. Plant traits were measured and analyzed regarding the growth and nutrition of both species. The results exhibited that the AM fungus significantly increased the height, diameter, biomass, C, N, and P acquisition of both the invasive E. adenophorum and the native E. lindleyanum. The root mycorrhizal colonization and the mycorrhizal dependency of native E. lindleyanum were greater than those of invasive E. adenophorum. Under M+, the Inter-competition inhibited the growth and nutrition of invasive E. adenophorum compared to the Intra- competition. Further, native E. lindleyanum exhibited higher competitiveness than invasive E. adenophorum in growth and nutrition. Meanwhile, the AM fungus significantly improved the competitiveness of native E. lindleyanum over invasive E. adenophorum. In conclusion, AM fungus improved the competitive advantage of native E. lindleyanum over invasive E. adenophorum in growth and nutrition, potentially contributing to native species competitively resisting the invasion of exotic species. These findings emphasize the importance of AM fungi in helping native plants resist the invasion of exotic plants and further contribute to understanding plant invasion prevention mechanisms.

10.
Clin. transl. oncol. (Print) ; 26(4): 797-807, Abr. 2024. ilus
Artigo em Inglês | IBECS | ID: ibc-VR-44

RESUMO

Tumor cells must resist the host's immune system while maintaining growth under harsh conditions of acidity and hypoxia, which indicates that tumors are more robust than normal tissue. Immunotherapeutic agents have little effect on solid tumors, mostly because of the tumor density and the difficulty of penetrating deeply into the tissue to achieve the theoretical therapeutic effect. Various therapeutic strategies targeting the tumor microenvironment (TME) have been developed. Immunometabolic disorders play a dominant role in treatment resistance at both the TME and host levels. Understanding immunometabolic factors and their treatment potential may be a way forward for tumor immunotherapy. Here, we summarize the metabolism of substances that affect tumor progression, the crosstalk between the TME and immunosuppression, and some potential tumor-site targets. We also summarize the progress and challenges of tumor immunotherapy.(AU)


Assuntos
Humanos , Masculino , Feminino , Imunoterapia , Metabolismo , Hipóxia , Microambiente Tumoral , Neoplasias/tratamento farmacológico
11.
J Colloid Interface Sci ; 666: 47-56, 2024 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-38583209

RESUMO

Solar light-driven hydrogen peroxide (H2O2) production through the two-electron oxygen reduction reaction (ORR) from the earth-abundant O2 and water is a potential alternative to the energy-consuming anthraquinone oxidation process, although the activity of the common photocatalysts is still insufficient to satisfy the industrial demands. Poor accessibility of O2 to surface/interface and fast carrier recombination is the limiting-factor for catalytic systems. Herein, we develop a nanohybrid photocatalysts by introducing 1D conducting polymer of polypyrrole (PPy) nanotube on In4SnS8 to promote H2O2 evolution under visible light, obtaining up to 254.8 µM in 2 h, which is 2.4- and 13-fold larger than that of individual In4SnS8 and PPy. The detailed characterizations of hybrid structure, O2 adsorption behaviors, charge carrier dynamics over PPy/In4SnS8 in conjunction with computational calculations corroborate that the modification of PPy could enlarge the amount of O2 adsorption amount, expedite the cycle of O2 adsorption/desorption and accelerate the transportation of electrons from In4SnS8 to the interface, eventually speeding up H2O2 photoproduction via indirect 2e- ORR pathway. This work establishes a paradigm of regulating the interfacial microenvironment by polymer for boosting H2O2 photogeneration through high selectivity of ORR.

12.
J Hazard Mater ; 470: 134156, 2024 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-38565015

RESUMO

While antimony (Sb) and arsenic (As) co-contamination in subsurface soil systems due to the legacy of Sb smelting wastes has been documented, the role of inherent heterogeneity on pollutant migration is largely overlooked. Herein this study investigated Sb and As migration in a slag impacted, vertically stratified subsurface at an abandoned Sb smelter. A 2-dimensional flume was assembled as a lab-scale analogue of the site and subject to rainfall and stop-rain events. Reactive transport modeling was then performed by matching the experimental observations to verify the key factors and processes controlling pollutant migration. Results showed that rainfall caused Sb and As release from the shallow slag layer and promoted their downward movement. Nevertheless, the less permeable deeper layers limited physical flow and transport, which led to Sb and As accumulation at the interface. The re-adsorption of Sb and As onto iron oxides in the deeper, more acidic layers further retarded their migration. Because of the large difference between Sb and As concentrations, Sb re-adsorption was much less effective, which led to higher mobility. Our findings overall highlight the necessity of understanding the degree and impacts of physicochemical heterogeneity for risk exposure assessment and remediation of abandoned Sb smelting sites.

13.
Sensors (Basel) ; 24(7)2024 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-38610395

RESUMO

Three-dimensional flexible piezoresistive porous sensors are of interest in health diagnosis and wearable devices. In this study, conductive porous sensors with complex triply periodic minimal surface (TPMS) structures were fabricated using the 3D printed sacrificial mold and enhancement of MWCNTs. A new curing routine by the self-resistance electric heating was implemented. The porous sensors were designed with different pore sizes and unit cell types of the TPMS (Diamond (D), Gyroid (G), and I-WP (I)). The impact of pore characteristics and the hybrid fabrication technique on the compressive properties and piezoresistive response of the developed porous sensors was studied. The results indicate that the porous sensors cured by the self-resistance electric heating could render a uniform temperature distribution in the composites and reduce the voids in the walls, exhibiting a higher elastic modulus and a better piezoresistive response. Among these specimens, the specimen with the D-based structure cured by self-resistance electric heating showed the highest responsive strain (61%), with a corresponding resistance response value of 0.97, which increased by 10.26% compared to the specimen heated by the external heat sources. This study provides a new perspective on design and fabrication of porous materials with piezoresistive functionalities, particularly in the realm of flexible and portable piezoresistive sensors.

14.
Int J Biol Macromol ; 266(Pt 1): 131399, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38641504

RESUMO

Developing an injectable hemostatic dressing with shape recovery and high blood absorption ratio for rapid hemostasis in noncompressible hemorrhage maintains a critical clinical challenge. Here, double-network cryogels based on carboxymethyl chitosan, sodium alginate, and methacrylated sodium alginate were prepared by covalent crosslinking and physical crosslinking, and named carboxymethyl chitosan/methacrylated sodium alginate (CM) cryogels. Covalent crosslinking was achieved by methacrylated sodium alginate in the freeze casting process, while physical crosslinking was realized by electrostatic interaction between the amino group of carboxymethyl chitosan and the carboxyl group of sodium alginate. CM cryogels exhibited large water swelling ratios (8167 ± 1062 %), fast blood absorption speed (2974 ± 669 % in 15 s), excellent compressive strength (over 160 kPa for CM100) and shape recovery performance. Compared with gauze and commercial gelatin sponge, better hemostatic capacities were demonstrated for CM cryogel with the minimum blood loss of 40.0 ± 8.9 mg and the lowest hemostasis time of 5.0 ± 2.0 s at hemostasis of rat liver. Made of natural polysaccharides with biocompatibility, hemocompatibility, and cytocompatibility, the CM cryogels exhibit shape recovery and high blood absorption rate, making them promising to be used as an injectable hemostatic dressing for rapid hemostasis in noncompressible hemorrhage.


Assuntos
Alginatos , Quitosana , Quitosana/análogos & derivados , Criogéis , Hemorragia , Hemostasia , Hemostáticos , Quitosana/química , Criogéis/química , Alginatos/química , Animais , Hemorragia/tratamento farmacológico , Ratos , Hemostasia/efeitos dos fármacos , Hemostáticos/química , Hemostáticos/farmacologia , Materiais Biocompatíveis/química , Humanos , Masculino
15.
Biosens Bioelectron ; 257: 116329, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38677023

RESUMO

Considerable effort has been invested in developing salicylic acid (SA) biosensors for various application purposes. Here, by engineering the sensing modules and host cell chassis, we have gradually optimized the NahR-Psal/Pr-based SA biosensor, increasing the sensitivity and maximum output by 17.2-fold and 9.4-fold, respectively, and improving the detection limit by 800-fold, from 80 µM to 0.1 µM. A portable SA sensing device was constructed by embedding a gelatin-based hydrogel containing an optimized biosensor into the perforations of tape adhered to glass slide, which allowed good determination of SA in the range of 0.1 µM-10 µM. Then, we developed a customized smartphone App to measure the fluorescence intensity of each perforation and automatically calculate the corresponding SA concentration so that we could detect SA concentrations in real cosmetic samples. We anticipate that this smartphone-based imaging biosensor, with its compact size, higher sensitivity, cost-effectiveness, and easy data transfer, will be useful for long-term monitoring of SA.


Assuntos
Técnicas Biossensoriais , Limite de Detecção , Ácido Salicílico , Smartphone , Técnicas Biossensoriais/instrumentação , Ácido Salicílico/análise , Ácido Salicílico/química , Desenho de Equipamento , Humanos , Hidrogéis/química , Cosméticos/química , Cosméticos/análise
16.
Gut Microbes ; 16(1): 2331435, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38502202

RESUMO

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a common food-borne pathogen that can cause acute diseases. Lysine acetylation is a post-translational modification (PTM) that occurs in various prokaryotes and is regulated by CobB, the only deacetylase found in bacteria. Here, we demonstrated that CobB plays an important role in the virulence of EHEC O157:H7 and that deletion of cobB significantly decreased the intestinal colonization ability of bacteria. Using acetylation proteomic studies, we systematically identified several proteins that could be regulated by CobB in EHEC O157:H7. Among these CobB substrates, we found that acetylation at the K44 site of CesA, a chaperone for the type-III secretion system (T3SS) translocator protein EspA, weakens its binding to EspA, thereby reducing the stability of this virulence factor; this PTM ultimately attenuating the virulence of EHEC O157:H7. Furthermore, we showed that deacetylation of the K44 site, which is deacetylated by CobB, promotes the interaction between CesA and EspA, thereby increasing bacterial virulence in vitro and in animal experiments. In summary, we showed that acetylation influences the virulence of EHEC O157:H7, and uncovered the mechanism by which CobB contributes to bacterial virulence based on the regulation of CesA deacetylation.


Assuntos
Infecções por Escherichia coli , Escherichia coli O157 , Proteínas de Escherichia coli , Microbioma Gastrointestinal , Animais , Escherichia coli O157/metabolismo , Virulência , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Proteômica , Infecções por Escherichia coli/microbiologia
17.
ACS Appl Mater Interfaces ; 16(12): 14626-14632, 2024 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-38477624

RESUMO

As one of the interesting signaling mechanisms, the in situ growth reaction on a photoelectrode has proven its powerful potential in photoelectrochemical (PEC) bioanalysis. However, the specific interaction between the signaling species with the photoactive materials limits the general application of the signal mechanism. Herein, on the basis of an in situ growth reaction on a photoelectrode of single-atom-based photoactive material, a general PEC immunoassay was developed in a split-type mode consisting of the immunoreaction and PEC detection procedure. Specifically, a single-atom photoactive material that incorporates Fe atoms into layered Bi4O5I2 (Bi4O5I2-Fe SAs) was used as a photoelectrode for PEC detection. The sandwich immunoreaction was performed in a well of a 96-well plate using Ag nanoparticles (Ag NPs) as signal tracers. In the PEC detection procedure, the Ag+ converted from Ag NPs were transferred onto the surface of the Bi4O5I2-Fe SAs photoelectrode and thereafter AgI was generated on the Bi4O5I2-Fe SAs in situ to form a heterojunction through the reaction of Ag+ with Bi4O5I2-Fe SAs. The formation of heterojunction greatly promoted the electro-hole separation, boosting the photocurrent response. Exemplified by myoglobin (Myo) as the analyte, the immunosensor achieved a wide linear range from 1.0 × 10-11 to 5.0 × 10-8 g mL-1 with a detection limit of 3.5 × 10-12 g mL-1. This strategy provides a general PEC immunoassay for disease-related proteins, as well as extends the application scope of in situ growth reaction in PEC analysis.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Técnicas Biossensoriais/métodos , Imunoensaio/métodos , Prata , Mioglobina , Técnicas Eletroquímicas/métodos , Limite de Detecção
18.
Skin Res Technol ; 30(3): e13616, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38424730

RESUMO

OBJECTIVE: To investigate the life, sleep quality and anxiety of rosacea patients in Yunnan and the improvement of these aspects after treatment. METHODS: A total of 141 patients with rosacea and 123 healthy controls were included in our study. The quality of life, sleep quality and anxiety of patients with rosacea and healthy controls were investigated by the Rosacea Severity Scores (RSSs), the Medical Outcomes Study 36-item short-form health survey (SF-36), the Pittsburgh Sleep Quality Index (PSQI) and Self-rating Anxiety Scale (SAS). The quality of life, sleep quality and anxiety of patients with rosacea were assessed again after treatment. RESULTS: Compared with healthy controls, patients with rosacea had significantly lower physical component scores (PCS) and mental component scores (MCS) but higher PSQI and SAS scores. After treatment, rosacea patients showed significantly higher MCS but lower PSQI and SAS scores. Correlation analysis showed a significant correlation between PCS, MCS, PSQI, SAS and RSSs. CONCLUSIONS: Patients with rosacea have a lower quality of life and sleep quality and tend to be more anxious than healthy controls. In addition, the mental quality of life, sleep quality and anxiety of rosacea patients can be significantly improved after treatment. Therefore, it is important to pay attention to the psychological status of rosacea patients. Psychological counseling and intervention are necessary to better prevent and treat rosacea.


Assuntos
Qualidade de Vida , Transtornos do Sono-Vigília , Humanos , Qualidade de Vida/psicologia , Estudos Retrospectivos , Qualidade do Sono , Transtornos do Sono-Vigília/psicologia , China/epidemiologia , Ansiedade/epidemiologia , Ansiedade/psicologia , Sono
19.
Appl Environ Microbiol ; 90(3): e0181823, 2024 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-38332488

RESUMO

Zearalenone (ZEN) and its derivatives are estrogenic mycotoxins known to pose significant health threats to humans and animals. Especially, the derivative α-zearalanol (α-ZAL) is over 10 times more toxic than ZEN. Simultaneous degradation of ZEN and its derivatives, especially α-ZAL, using ZEN lactone hydrolases (ZHDs) is a promising solution to eliminate their potential hazards to food safety. However, most available ZHDs exhibit limited activity toward the more toxic α-ZAL compared to ZEN. Here, we identified a broad-substrate spectrum ZHD, named ZHDAY3, from Exophiala aquamarina CBS 119918, which could not only efficiently degrade ZEN but also exhibited 73% relative activity toward α-ZAL. Through rational design, we obtained the ZHDAY3(N153H) mutant, which exhibited the highest specific activity (253.3 ± 4.3 U/mg) reported so far for degrading α-ZAL. Molecular docking, structural comparative analysis, and kinetic analysis collectively suggested that the shorter distance between the side chain of the catalytic residue His242 and the lactone bond of α-ZAL and the increased binding affinity to the substrate were mainly responsible for the improved catalytic activity of ZHDAY3(N153H) mutant. This mechanism was further validated through additional molecular docking of 18 mutants and experimental verification of six mutants.IMPORTANCEThe mycotoxins zearalenone (ZEN) and its derivatives pose a significant threat to food safety. Here, we present a highly promising ZEN lactone hydrolase (ZHD), ZHDAY3, which is capable of efficiently degrading both ZEN and the more toxic derivative α-ZAL. Next, the ZHDAY3(N153H) mutant obtained by single-point mutation exhibited the highest specific activity for degrading α-ZAL reported thus far. We further elucidated the molecular mechanisms underlying the enhanced hydrolytic activity of ZHDAY3(N153H) toward α-ZAL. These findings represent the first investigation on the molecular mechanism of ZHDs against α-ZAL and are expected to provide a significant reference for further rational engineering of ZHDs, which will ultimately contribute to addressing the health risks and food safety issues posed by ZEN-like mycotoxins.


Assuntos
Micotoxinas , Zearalenona , Zeranol , Humanos , Animais , Zearalenona/química , Zearalenona/metabolismo , Zeranol/química , Zeranol/metabolismo , Lactonas , Mutação Puntual , Hidrolases/metabolismo , Simulação de Acoplamento Molecular , Cinética , Micotoxinas/metabolismo
20.
Adv Mater ; 36(23): e2313090, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38385793

RESUMO

In the last decade, interfacial solar steam generation (ISSG), powered by natural sunlight garnered significant attention due to its great potential for low-cost and environmentally friendly clean water production in alignment with the global decarbonization efforts. This review aims to share the knowledge and engage with a broader readership about the current progress of ISSG technology and the facing challenges to promote further advancements toward practical applications. The first part of this review assesses the current strategies for enhancing the energy efficiency of ISSG systems, including optimizing light absorption, reducing energy losses, harvesting additional energy, and lowering evaporation enthalpy. Subsequently, the current challenges faced by ISSG technologies, notably salt accumulation and bio-fouling issues in practical applications, are elucidated and contemporary methods are discussed to overcome these challenges. In the end, potential applications of ISSG, ranging from initial seawater desalination and industrial wastewater purification to power generation, sterilization, soil remediation, and innovative concept of solar sea farm, are introduced, highlighting the promising potential of ISSG technology in contributing to sustainable and environmentally conscious practices. Based on the review and in-depth understanding of these aspects, the future research focuses are proposed to address potential issues in both fundamental research and practical applications.

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