[Evaluation of the clinical effect of fluoride coating combined with pit and fissure sealing or preventive resin filling on prevention of first permanent molars caries].

PURPOSE: To explore the clinical effect of fluoride coating combined with pit and fissure sealing or preventive resin filling on prevention of young first permanent molars caries. METHODS: Three hundred suspicious first permanent molars caries in 90 children (6-8 years old) who received oral health examination in our hospital from February 2015 to January 2016 were included as the study subject, and randomly divided into group A, B, C, D and E. Group A received pit and fissure sealing, group B received preventive resin filling, group C received 0.5% fluoride coating combined with pit and fissure sealing, group D received 0.5% fluoride combined with preventive resin filling, group E as control group with nursing daily brushing. 6, 12 and 18 months after treatment, caries rate in each group was evaluated and preservation of pit and fissure sealing or resin filling in group A, B, C, D was examined. The data were analyzed using SPSS 21.0 software package. RESULTS: Six and 12 months after treatment, there was no significant difference (P>0.05) in the preservation rate of pit and fissure sealing or resin filling and caries rate in group A, B, C, D. Eighteen months after treatment, caries rate in group E was significantly higher than that of group A, B, C, D; there was significant difference between group A and group C, group B and group D (P<0.01); caries rate of group A, B was relatively high, the preservation rate was lower than that of group C and D, the differences were statistically significant (P<0.05). CONCLUSIONS: For suspected young permanent molars caries, fluoride coating combined with pit and fissure sealing or separate pit and fissure sealing has a certain effect on prevent dental caries, but the fluoride coating combined with preventive resin filling is better.

Killer cell immunoglobulin-like receptor gene diversity in the Tibetan ethnic minority group of China.

The aim of this study was to analyze killer immunoglobulin-like receptor (KIR) gene polymorphisms in the Tibetan ethnic minority of China. To that purpose, we have studied KIR gene frequencies and genotype diversities of 16 KIR genes and three pseudogenes (2DL1, 2DL2, 2DL3, 2DL4, 2DL5A, 2DL5B, 2DS1, 2DS2, 2DS3, 2DS4*001/002, 2DS4*003-007, 2DS5, 3DL1, 3DL2, 3DL3, 3DS1, 2DP1, 3DP1*001/002/004, and 3DP1*003) in a population sample of 102 unrelated healthy individuals of the Tibetan population living in Lhasa city, Tibet Autonomous Region of China. Tibetans mainly live in "the roof of the world," the Qinghai-Tibet Plateau of China and surrounding areas stretching from central Asia in the North and West to Myanmar and mainland China in the East, and India, Nepal, and Bhutan to the south. KIR gene frequencies and statistical parameters of Tibetan ethnic minority were calculated. Fifteen KIR genes were observed in the 102 tested Tibetan individuals with different frequencies. The allelic frequencies of the 15 KIR genes ranged from 0.06 to 0.86. In addition, KIR 2DL1, 2DL4, 3DL2, and 3DL3 were found to be present in every individual. Variable gene content, together with allelic polymorphisms, can result in individualized human KIR genotypes and haplotypes, with the A haplotypes being predominantly observed. The results of tested linkage disequilibrium (LD) among KIR genes demonstrated that KIR genes present a wide range of linkage disequilibrium. Moreover, a comparison of the population data of our study with previously published population data of other ethnic groups or areas was performed. The differences of allelic frequency distribution in KIR2DL2, 2DL3, 2DL5, 3DL1, 2DS1, 2DS2, 2DS3, 3DS1, and 2DP1 were statistically significant among different populations using the statistical method of the standard χ(2) test. In conclusion, the results of the present study can be valuable for enriching the Chinese ethnical gene information resources of the KIR gene pool and for anthological studies, as well as for KIR-related disease research.

Population data of 15 STR loci of Chinese Yi ethnic minority group.

Allele frequency data and statistical parameters for D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA loci were determined in a sample of 120 healthy unrelated individuals of Chinese Yi ethnic minority group living in Yunnan province, China. We observed 132 alleles with allelic frequencies ranging from 0.0042 to 0.5333. The forensic statistical parameters from the data of all the loci showed high values. All loci were in accordance with Hardy-Weinberg equilibrium (p>0.05). The obtained frequency distributions were compared with previously published other population data, and significant differences were found between Yi population and Korean, Chinese Tibetan, Uigur, Ewenki, Han, Hui population at some STR loci. Our results of present study were valuable for forensic application and Chinese population genetic studies. These population data enriched Chinese genetic informational resources.