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1.
Transl Res ; 268: 13-27, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38286358

RESUMO

Inflammation is a crucial pathophysiological mechanism in atherosclerosis (AS). This study aims to investigate the impact of sulfotransferase family 2b member 1 (SULT2B1) on the inflammatory response of macrophages and the progression of AS. Here, we reported that SULT2B1 expression increased with the progression of AS. In AS model mice, knockdown of Sult2b1 led to remission of AS and reduced inflammation levels. Further exploration of the downstream molecular mechanisms of SULT2B1 revealed that suppressing Sult2b1 in macrophages resulted in decreased levels of 25HC3S in the nucleus, elevated expression of Lxr, and increased the transcription of Lncgga3-204. In vivo, knockdown of Lncgga3-204 aggravated the inflammatory response and AS progression, while the simultaneous knockdown of both Sult2b1 and Lncgga3-204 exacerbated AS and the inflammatory response compared with knockdown of Sult2b1 alone. Increased binding of Lncgga3-204 to SMAD4 in response to oxidized-low density lipoprotein (ox-LDL) stimulation facilitated SMAD4 entry into the nucleus and regulated Smad7 transcription, which elevated SMAD7 expression, suppressed NF-κB entry into the nucleus, and ultimately attenuated the macrophage inflammatory response. Finally, we identified the presence of a single nucleotide polymorphism (SNP), rs2665580, in the SULT2B1 promoter region in monocytes from coronary artery disease (CAD) patients. The predominant GG/AG/AA genotypes were observed in the Asian population. Elevated SULT2B1 expression in monocytes with GG corresponded to elevated inflammatory factor levels and more unstable coronary plaques. To summarize, our study demonstrated that the critical role of SULT2B1/Lncgga3-204/SMAD4/NF-κB in AS progression. SULT2B1 serves as a novel biomarker indicating inflammatory status, thereby offering insights into potential therapeutic strategies for AS.


Assuntos
Aterosclerose , Progressão da Doença , Inflamação , Macrófagos , Proteína Smad4 , Sulfotransferases , Aterosclerose/metabolismo , Aterosclerose/genética , Aterosclerose/patologia , Sulfotransferases/genética , Sulfotransferases/metabolismo , Animais , Camundongos , Macrófagos/metabolismo , Humanos , Inflamação/metabolismo , Inflamação/patologia , Proteína Smad4/metabolismo , Proteína Smad4/genética , Masculino , Camundongos Endogâmicos C57BL , Feminino
2.
Angew Chem Int Ed Engl ; 63(6): e202318792, 2024 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-38117669

RESUMO

Electroreduction of nitric oxide (NO) to NH3 (NORR) has gained extensive attention for the sake of low carbon emission and air pollutant treatment. Unfortunately, NORR is greatly hindered by its sluggish kinetics, especially under low concentrations of NO. Herein, we developed a chlorine (Cl) vacancy strategy to overcome this limitation over FeOCl nanosheets (FeOCl-VCl ). Density functional theory (DFT) calculations revealed that the Cl vacancy resulted in defective Fe with sharp d-states characteristics in FeOCl-VCl to enhance the absorption and activation of NO. In situ X-ray absorption near-edge structure (XANES) and attenuated total reflection-infrared spectroscopy (ATR-IR) verified the lower average oxidation state of defective Fe to enhance the electron transfer for NO adsorption/activation and facilitate the generation of key NHO and NHx intermediates. As a result, the FeOCl-VCl exhibited superior NORR activities with the NH3 Faradaic efficiency up to 91.1 % while maintaining a high NH3 yield rate of 455.4 µg cm-2 h-1 under 1.0 vol % NO concentration, competitive with those of previously reported literatures under higher NO concentration. Further, the assembled Zn-NO battery utilizing FeOCl-VCl as cathode delivered a record peak power density of 6.2 mW cm-2 , offering a new route for simultaneous NO removal, NH3 production, and energy supply.

3.
Small ; 19(38): e2301433, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37263991

RESUMO

Lithium metal batteries are intensively studied due to the potential to bring up breakthroughs in high energy density devices. However, the inevitable growth of dendrites will cause the rapid failure of battery especially under high current density. Herein, the utilization of tetrachloroethylene (C2 Cl4 ) is reported as the electrolyte additive to induce the formation of the LiCl-rich solid electrolyte interphase (SEI). Because of the lower Li ion diffusion barrier of LiCl, such SEI layer can supply sufficient pathway for rapid Li ion transport, alleviate the concentration polarization at the interface and inhibit the growth of Li dendrites. Meanwhile, the C2 Cl4 can be continuously replenished during the cycle to ensure the stability of the SEI layer. With the aid of C2 Cl4 -based electrolyte, the Li metal electrodes can maintain stable for >300 h under high current density of 50 mA cm-2 with areal capacity of 5 mAh cm-2 , broadening the compatibility of lithium metal anode toward practical application scenarios.

4.
Clin Transl Med ; 13(3): e1220, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36932468

RESUMO

BACKGROUND: Atherosclerosis (AS), the main pathological basis of life-threatening cardiovascular disease, is essentially caused by chronic macrophage inflammation. Overexpression of proline/serine-rich coiled-coil protein 1 (PSRC1) reduces macrophage inflammatory responses and delays AS development. However, the exact mechanism of PSRC1 is unclear. METHODS: Proteins interacting with PSRC1 were screened by proteomics in RAW264.7 cells, followed by RT-qPCR, immunoprecipitation and immunofluorescence to explore the specific mechanistic pathways affecting inflammation. CRISPR-Cas9 constructs for PSRC1-/- ApoE-/- (DKO) mice and high-fat diet-fed ApoE-/- and DKO mice were used for AS models for in vivo experiments. Upstream transcription factors of PSRC1 were predicted by ATAC-seq, ChIP-seq and UCSC, and the regulatory mechanism was verified by ChIP-qPCR and dual luciferase assays. Peripheral blood serum and monocytes were collected from coronary artery disease (CAD) patients and non-CAD patients. RESULTS: Increased binding of ANXA2 to PSRC1 in macrophages under oxidized low-density lipoprotein stimulation and decreased release of ANXA2 to the extracellular compartment were observed. Knockdown of ANXA2 in AS model mice delayed AS progression. Knockdown of ANXA2 in DKO mice reversed the AS-promoting effect of PSRC1 knockdown. Mechanistically, ANXA2 promotes STAT3 phosphorylation, which in turn promotes inflammatory responses. In addition, SP1 is a PSRC1 upstream repressive transcription factor, and the SP1 inhibitor mithramycin (Mith) elevated PSRC1 expression and exerted anti-AS effects in AS model mice. Patients with CAD had considerably greater serum levels of ANXA2 than those without CAD, and Mith reduced the secretion of ANXA2 in peripheral blood monocytes of CAD patients. CONCLUSION: In macrophages, PSRC1 can interact with ANXA2 to inhibit its extracellular release and delay AS development. SP1 is an upstream transcription factor of PSRC1 and inhibits the transcription of PSRC1. The SP1 inhibitor Mith can elevate PSRC1 levels and slow AS progression while reducing ANXA2 release from monocytes in CAD patients. Mith is expected to be a new agent for AS treatment.


Assuntos
Anexina A2 , Aterosclerose , Doença da Artéria Coronariana , Fosfoproteínas , Animais , Camundongos , Aterosclerose/metabolismo , Inflamação , Macrófagos/metabolismo , Prolina , Serina , Fatores de Transcrição/metabolismo , Fosfoproteínas/metabolismo , Camundongos Knockout para ApoE
5.
Small ; 18(51): e2205470, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36328710

RESUMO

Lithium-sulfur (Li-S) batteries have attracted great interest due to their low cost, high theoretical energy density, and environmental friendliness. However, the sluggish conversion of lithium polysulfides (LiPS) to S and Li2 S during the charge/discharge process leads to unsatisfactory rate performance of lower to 0.1 C (1 C = 1675 mA g-1 ) especially for Li-S pouch batteries, thus hindering their practical applications in high power batteries. Here, well-defined and monodispersed Ni single-atom catalysts (SACs) embedded in highly porous nitrogen-doped graphitic carbons (NiSA-N-PGC) are designed and synthesized to form Ni-N4 catalytic sites at the atomic level. When serving as a bifunctional electrocatalyst, the Ni-N4 catalytic sites cannot only promote the interfacial conversion redox of LiPS by accelerating the transformation kinetics, but also suppress the undesirable shuttle effect by immobilizing LiPS. These findings are verified by both experimental results and DFT theoretical calculations. Furthermore, Li ions show low diffusion barrier on the surface of Ni-N4 sites, resulting in enhanced areal capacity of batteries. As a result, the Li-S battery delivers stable cycling life of more than 600 cycles with 0.069% capacity decay per cycle at a rate of 0.5 C. More importantly, the Li-S pouch cells with NiSA-N-PGC show an initial capacity of 1299 mAh g-1 at a rate of 0.2 C even with high sulfur loading of 6 mg cm-2 . This work opens up an avenue for developing single-atom catalysts to accelerate the kinetic conversion of LiPS for highly stable Li-S batteries.

6.
Front Immunol ; 13: 900334, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35874761

RESUMO

Coronary atherosclerotic heart disease (CAD) is a chronic inflammatory cardiovascular disease with high morbidity and mortality. Growing data indicate that many immune cells are involved in the development of atherosclerosis. However, the immunological roles of γδ T cells in the initiation and progression of CAD are not fully understood. Here, we used flow cytometry to determine phenotypical changes of γδ T cells and their subpopulations in peripheral blood samples collected from 37 CAD patients. The Pearson correlation coefficient was used to analyze the relationship between the clinical parameter (serum LDL-C level) and the changes of immunophenotypes of γδ T cells. Our results demonstrated that the frequencies and absolute numbers of total γδ T cells and Vδ2+ T cells were significantly decreased in CAD patients when compared to healthy individuals. However, the proportion of Vδ1+ T cells was much lower in CAD patients than that of healthy individuals. Most importantly, a significant alteration of the Vδ1/Vδ2 ratio was found in CAD patients. In addition, a series of surface markers that are associated with costimulatory signals (CD28, CD40L, CD80, CD86), activation levels (CD69, CD25, HLA-DR), activating NK cell receptors (NKp30, NKp46, NKG2D) and inhibitory receptors (PD-1, CTLA-4, PD-1, Tim-3) were determined and then analyzed in the total γδ T cells, Vδ2+T cells and Vδ2-T cells of CAD patients and healthy individuals. The data demonstrated that immunological activities of total γδ T cells, Vδ2+T cells, and Vδ2-T cells of CAD patients were much lower than those in healthy individuals. Moreover, we found that there were positive correlations between the serum LDL-C levels and frequencies of CD3+γδ+ T cells, CD69+Vδ2+T cells, NKG2D+Vδ2+T cells, and NKp46+Vδ2+T cells. By contrast, there was an inverse correlation between the levels of serum LDL-C and the frequencies of CD69+Vδ2-T cells and NKp46+Vδ2-T cells. Accordingly, these findings could help us to better understand the roles of γδ T cells in the CAD, and shed light on the development of novel diagnostic techniques and therapeutic strategies by targeting γδ T cells for CAD patients.


Assuntos
Doença da Artéria Coronariana , Receptores de Antígenos de Linfócitos T gama-delta , LDL-Colesterol/metabolismo , Doença da Artéria Coronariana/metabolismo , Humanos , Subfamília K de Receptores Semelhantes a Lectina de Células NK/metabolismo , Receptor de Morte Celular Programada 1/metabolismo , Receptores de Antígenos de Linfócitos T gama-delta/metabolismo , Subpopulações de Linfócitos T
7.
Phys Chem Chem Phys ; 24(8): 4891-4898, 2022 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-35137755

RESUMO

The unique structures of polynuclear MoxSy clusters make it possible to maximize the number of their active sites and for them to be good candidates for HER catalysts. An appropriate support is highly necessary not only to avoid the desorption of MoxSy clusters in a working environment, but also to improve their HER activity. Our work here shows that the CeO2 support could provide strong support for interaction with various MoxSy clusters and the formed MoxSy/CeO2 hetero-structures also have modest ΔGH* for the HER. The electronic features of MoxSy clusters are regulated by the CeO2 support, which leads to charge redistribution on edge atoms and plays a key role in H adsorption. Our studies provide instructive predictions on efficient candidates of molybdenum-sulfur based catalysts for the HER.

8.
J Phys Condens Matter ; 34(4)2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34678791

RESUMO

Electrochemical N2reduction reaction (NRR) for NH3synthesis, which usually needs highly-efficient electrocatalysts for N2activation, is a carbon-neutral alternation compared to the traditional Haber-Bosch process. Although Ti-based compounds is widely used as electrocatalysts, what Ti defect affects NRR activity is still illusive. In this work, our systematic density functional calculations on Ti defect-decorated titanium oxide disclose that the unsaturated-Ti with the orbital splitting of defect electron states is the necessary feature for N2binding and activation, which can be further enhanced by increasing the splitting degree. The bonding/antibonding orbital population and projected density of states indicate that the nature of N2binding and activation on Ti-defect site is attributed to the elimination of the bonding orbital population in the conduction bands and the formation of*πback-bonding in the valence bands. For the whole NRR process, the synergy of Ti-defect and oxygen vacancy (VO) promotes N2reduction, and the required maximum energy input scales quite well with the adsorption strength of*NNH. Finally, the formed volcano shape successfully predicts new candidate catalysts for ammonia synthesis, such as TiO2combined VOwith Ti interstitial or H atom. This work provides disclosure of the key elements on the rational design of Ti-based nanomaterial electrocatalysts for artificial N2fixation.

9.
Angew Chem Int Ed Engl ; 60(41): 22513-22521, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34387407

RESUMO

We synthesized a series of carbon-supported atomic metal-N-C catalysts (M-SACs: M=Mn, Fe, Co, Ni, Cu) with similar structural and physicochemical properties to uncover their catalytic activity trends and mechanisms. The peroxymonosulfate (PMS) catalytic activity trends are Fe-SAC>Co-SAC>Mn-SAC>Ni-SAC>Cu-SAC, and Fe-SAC displays the best single-site kinetic value (1.65×105  min-1 mol-1 ) compared to the other metal-N-C species. First-principles calculations indicate that the most reasonable reaction pathway for 1 O2 production is PMS→OH*→O*→1 O2 ; M-SACs that exhibit moderate and near-average Gibbs free energies in each reaction step have a better catalytic activity, which is the key for the outstanding performance of Fe-SACs. This study gives the atomic-scale understanding of fundamental catalytic trends and mechanisms of PMS-assisted reactive oxygen species production via M-SACs, thus providing guidance for developing M-SACs for catalytic organic pollutant degradation.

10.
Aging (Albany NY) ; 13(3): 3428-3442, 2021 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-33428590

RESUMO

Atherosclerosis is a lipid-driven chronic inflammatory disease in which lipid-laden macrophage foam cells lead to inflamed lesions in arteries. Previous studies have proven that sulfotransferase 2B1b (SULT2B1b) has several roles in the regulation of lipid metabolism and the inflammatory response. However, little is known about the functions of SULT2B1b in ox-LDL-induced inflammation in macrophages. In this study, after treatment with either ox-LDL alone or combined with transfection of siRNAs targeting SULT2B1b, IL-6, TNF-α, NF-κB, IKKß and IκB mRNA and protein expression were determined in Raw264.7 cells by real-time PCR and Western blot, respectively. The proliferative capacity was determined by EdU staining and Cell Counting Kit-8. Our data demonstrated that SULT2B1b knockdown could reduce phosphorylated NF-κB levels and downregulate IKKß protein levels. Additionally, IκB levels were increased and the proliferation of ox-LDL stimulated cells was inhibited after SULT2B1b silencing. Downregulation of SULT2B1b expression was found to upregulate miR-148a-3p expression by microarray assay, while IKKß was a miR-148a-3p target gene. Our study suggests that SULT2B1b knockdown could promote miR148a-3p expression and inhibit activation of the IKKß/NF-κB signalling pathway, which suppressed the inflammatory response in macrophages. Therefore, targeting the SULT2B1b gene might be potentially beneficial for atherosclerosis prevention by decreasing the inflammatory response.


Assuntos
Quinase I-kappa B/genética , Inflamação/genética , Lipoproteínas LDL/imunologia , Macrófagos/metabolismo , MicroRNAs/genética , NF-kappa B/genética , Sulfotransferases/genética , Animais , Aterosclerose/imunologia , Proliferação de Células , Técnicas de Silenciamento de Genes , Quinase I-kappa B/imunologia , Inflamação/imunologia , Metabolismo dos Lipídeos/genética , Metabolismo dos Lipídeos/imunologia , Macrófagos/imunologia , Camundongos , NF-kappa B/imunologia , Células RAW 264.7 , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Sulfotransferases/imunologia
11.
Adv Mater ; 32(30): e2000299, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32567074

RESUMO

The ambient electrocatalytic N2 reduction reaction (NRR) enabled by TiO2 has attracted extensive recent attention. Previous studies suggest the formation of Ti3+ in TiO2 can significantly improve the NRR activity, but it still remains unclear what kinds of Ti3+ are effective. Herein, it is demonstrated that mixed-valent Cu acts as an effective dopant to modulate the oxygen vacancy (VO ) concentration and Ti3+ formation, which markedly improves the electrocatalytic NRR performance. In 0.5 m LiClO4 , this electrocatalyst attains a high Faradic efficiency of 21.99% and a large NH3 yield of 21.31 µg h-1 mgcat. -1 at -0.55 V vs reversible hydrogen electrode, which even surpasses most reported Ti-based NRR electrocatalysts. Using density function theory calculations, it is evidenced that mixed-valent Cu ions modulate the TiO2 (101) surface with multiple oxygen vacancies, which is beneficial for generating different Ti3+ 3d1 defect states localized below the Fermi energy. N2 activation and adsorption are effectively strengthened when Ti3+ 3d1 defect states present the splitting of eg and t2g orbitals, which can be modulated by its coordination structure. The synergistic roles of the three ion pairs formed by the VO defect, including Cu1+ -Ti4+ , Ti3+ -Ti4+ and Ti3+ -Ti3+ , are together responsible for the enhanced NRR performance.

12.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(3): 382-387, 2020 Mar 30.
Artigo em Chinês | MEDLINE | ID: mdl-32376582

RESUMO

OBJECTIVE: To analyze the correlation of annexin A2 with coronary atherosclerotic heart disease (CAD) and the severity of CAD. METHODS: We collected data from a total of 200 inpatients admitted in our department between August, 2017 and August, 2019. According to the. RESULTS: of coronary angiography, the patients were divided into CAD group (n=150) and non-CAD (n=50), and the CAD patients was further divided, according to their clinical stability, into stable angina (SAP) group and acute coronary syndrome (ACS) group. Serum levels of annexin A2, MPO and PON1 were detected in all these patients, and their correlations with CAD, disease severity, and degree of coronary artery stenosis were analyzed.ResultsThe levels of annexin A2 and MPO were significantly higher in CAD patients than in non-CAD patients (P < 0.05). Among the CAD patients, those with ACS had significantly higher levels of annexin A2 (P < 0.05) and lower levels of PON-1 (P < 0.05) than those with SAP, but annexin A2 level was not significantly correlated with coronary lesion count, Gensini score, or the co-morbidity of diabetes. CONCLUSIONS: Annexin A2 is significantly elevated in patients with CAD, especially in those with ACS, and can be used as a predictor of clinical instability.


Assuntos
Anexina A2/sangue , Doença da Artéria Coronariana , Arildialquilfosfatase , Biomarcadores , Angiografia Coronária , Feminino , Humanos , Masculino , Índice de Gravidade de Doença
13.
BMC Public Health ; 20(1): 263, 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32085727

RESUMO

BACKGROUND: In developing countries, ambient sulfur dioxide (SO2) is a serious air pollutant concern, but there is no enough and consistent epidemiological evidence about its health effects on stroke hospitalization. METHODS: We collected the daily air pollution data, meteorological data and number of daily hospital admissions for ischemic and hemorrhagic stroke, in Guangzhou from January 1st 2009 to December 31st 2014. Then we applied generalized additive model with a quasi-Poisson link to assess the relationship between short-term SO2 exposure and the total number of hospital admissions for ischemic and hemorrhagic stroke. In addition, we evaluated the effect of ambient SO2 by age (< 65 years and ≥ 65 years). RESULTS: During the study period, a 24-h mean concentration of ambient SO2 of 27.82 µg/m3, a total of 58,473 ischemic stroke and 9167 hemorrhagic stroke hospital admissions hospital were recorded. Ambient SO2 was found to increase the risk for both ischemic and hemorrhagic stroke hospital admission in single pollutant model. The maximum value of percentage changes for ischemic and hemorrhagic stroke occurred in lag 0 day and lag 1 day, per 10 µg/m3 increase in SO2 concentrations was corresponded to a 1.27% (95% confidence interval (CI), 0.42-2.12%) and 1.55% (95%CI, 0.02-3.11%) increased risk, respectively. The association between SO2 and ischemic stroke hospitalization was robust to two pollutant model, but for hemorrhagic stroke it's partially weakened after adjusting for co-pollutants. The effect of ambient SO2 on ischemic stroke appeared to be greater for people < 65 years old, but null effect on hemorrhagic stroke was identified for both age groups. CONCLUSIONS: We found short-term exposure to ambient SO2 may significantly increase the risks of hospitalization for ischemic stroke. The findings may contribute to a better understanding of the health effects of low-levels of SO2.


Assuntos
Poluição do Ar/efeitos adversos , Exposição Ambiental/estatística & dados numéricos , Hospitalização/estatística & dados numéricos , Acidente Vascular Cerebral/terapia , Dióxido de Enxofre/efeitos adversos , Idoso , Poluição do Ar/análise , China/epidemiologia , Humanos , Pessoa de Meia-Idade , Medição de Risco , Acidente Vascular Cerebral/epidemiologia , Dióxido de Enxofre/análise
14.
J Mol Cell Cardiol ; 139: 87-97, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31972265

RESUMO

Lots of studies demonstrated that CD4+ T cells regulate the development of atherosclerosis (AS). Previously, we reported that LCK, a key molecule in activation of T cell receptor (TCR) signalling and T cells, adversely affects reverse cholesterol transport (RCT), which ameliorates AS in vitro. To investigate the effect of LCK on AS in vivo, we injected the LCK inhibitor, PP2, into ApoE-/- mice fed a chow diet or a high-fat diet (HFD). Although, AS plaques were not affected by PP2 in chow diet-fed mice, PP2 significantly reduced the lesion percentage and necrotic core areas in HFD-fed mice. We further analysed the plaque contents and found that the accumulation of lipids and macrophages were decreased, while the contents of collagen and smooth muscle cells were increased by the LCK inhibitor. Thus, inhibiting LCK enhanced the plaque stability. We also found the LCK inhibitor improved cholesterol efflux capacity of HDL and up-regulated RCT regulatory proteins in the spleen. Moreover, inhibiting LCK regulated differentiation of T cells by increasing regulatory T (Treg) cells and decreasing the number of T helper 1 (Th1) cells in the aorta, thymus and spleen. Consistent with these results, infiltration of CD4+ T cells in plaques, secretion of pro-atherosclerotic cytokines, INF-γ and TNF-α synthesized mostly by Th1 cells, and the activation of PI3K/AKT/mTOR signalling were inhibited by the LCK inhibitor. Moreover, the effect of LCK inhibitor on the ratio of Th1 to Treg cells were compromised by activation of mTOR. Together, these data indicate that inhibiting LCK in TCR signalling attenuated the development of AS and promoted plaque stability. Improving RCT by upregulating RCT regulatory proteins and decreasing the Th1/Treg ratio by inhibiting PI3K/AKT/mTOR signalling may contribute to the anti-atherosclerotic effects of LCK inhibition.


Assuntos
Apolipoproteínas E/deficiência , Diferenciação Celular , Colesterol/metabolismo , Proteína Tirosina Quinase p56(lck) Linfócito-Específica/antagonistas & inibidores , Linfócitos T/citologia , Linfócitos T/metabolismo , Animais , Aterosclerose/sangue , Aterosclerose/metabolismo , Aterosclerose/patologia , Transporte Biológico/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Dieta Hiperlipídica , Lipídeos/sangue , Proteína Tirosina Quinase p56(lck) Linfócito-Específica/metabolismo , Masculino , Camundongos , Modelos Biológicos , Necrose , Fosfatidilinositol 3-Quinases/metabolismo , Placa Aterosclerótica/sangue , Placa Aterosclerótica/patologia , Inibidores de Proteínas Quinases/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Células RAW 264.7 , Transdução de Sinais/efeitos dos fármacos , Linfócitos T/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismo , Regulação para Cima/efeitos dos fármacos
15.
Chem Commun (Camb) ; 56(12): 1831-1834, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-31950935

RESUMO

Catalysts for the N2 reduction reaction (NRR) are at the heart of key alternative technology to the Haber-Bosch process for NH3 synthesis, and are expected to optimize the interplay between efficiency, activity and selectivity. Here, we report our recent finding that P-doped graphene shows superior NRR performances in aqueous media at present, with a remarkably large NH3 yield of 32.33 µg h-1 mgcat.-1 and a high faradaic efficiency of 20.82% at -0.65 V vs. reversible hydrogen electrode. The mechanism is clarified by density functional theory calculations.

16.
Clin Sci (Lond) ; 134(2): 273-287, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-31957803

RESUMO

The current main treatment for coronary artery disease (CAD) is to reduce low-density lipoprotein cholesterol (LDL-C) by statins, which could decrease the incidence of major adverse cardiovascular events (MACEs) by 30%. However, many residual risks still remain. To clarify the mechanism involved, we studied patients with acute myocardial infarction (AMI) with low LDL-C levels. Lymphocytes were isolated, and it was found that despite no difference in plasma LDL-C level, the lymphocyte cholesterol content was higher in AMI patient than those in non-CAD patients; thus, the decrease in intracellular cholesterol content was inconsistent with that in the plasma. Additionally, [3H]-cholesterol efflux rates were lower and mRNA levels of the inflammatory factors tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) higher in AMI lymphocytes. It was found that sulphotransferase 2B1b (SULT2B1b) expression was higher in AMI lymphocytes. Further research using Jurkat T lymphocytes confirmed that SULT2B1b knockdown increased cholesterol efflux capacity and decreased mRNA levels of TNF-α and IFN-γ by increasing liver X receptor (LXR)-ß levels. Furthermore, the degree of CpG island methylation in the SULT2B1b promoter was reduced in cells from AMI patients. In conclusion, SULT2B1b up-regulation due to hypomethylation of its promoter promotes cholesterol accumulation and inflammation by inhibiting LXR-ß in lymphocytes of AMI patients with low LDL-C levels. Therefore, reducing intracellular cholesterol is also important as plasma cholesterol levels. Therapeutic approaches to decrease SULT2B1b expression might be potentially beneficial for CAD prevention by decreasing intracellular cholesterol.


Assuntos
Colesterol/metabolismo , Interferon gama/metabolismo , Linfócitos/metabolismo , Sulfotransferases/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Transporte Biológico , Colesterol/sangue , LDL-Colesterol/metabolismo , Doença da Artéria Coronariana/genética , Doença da Artéria Coronariana/metabolismo , Doença da Artéria Coronariana/prevenção & controle , Metilação de DNA , Humanos , Mediadores da Inflamação/metabolismo , Interferon gama/genética , Células Jurkat , Receptores X do Fígado/genética , Receptores X do Fígado/metabolismo , Infarto do Miocárdio/genética , Infarto do Miocárdio/metabolismo , Infarto do Miocárdio/prevenção & controle , Regiões Promotoras Genéticas/genética , Sulfotransferases/genética , Fator de Necrose Tumoral alfa/genética
17.
Angew Chem Int Ed Engl ; 59(2): 758-762, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31664770

RESUMO

Electrochemical reduction of CO2 into various chemicals and fuels provides an attractive pathway for environmental and energy sustainability. It is now shown that a FeP nanoarray on Ti mesh (FeP NA/TM) acts as an efficient 3D catalyst electrode for the CO2 reduction reaction to convert CO2 into alcohols with high selectivity. In 0.5 m KHCO3 , such FeP NA/TM is capable of achieving a high Faradaic efficiency (FE CH 3 OH ) up to 80.2 %, with a total FE CH 3 OH + C 2 H 5 OH of 94.3 % at -0.20 V vs. reversible hydrogen electrode. Density functional theory calculations reveal that the FeP(211) surface significantly promotes the adsorption and reduction of CO2 toward CH3 OH owing to the synergistic effect of two adjacent Fe atoms, and the potential-determining step is the hydrogenation process of *CO.

18.
Chem Commun (Camb) ; 56(5): 731-734, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31840689

RESUMO

Fe-enabled high-performance ambient electrochemical N2 reduction still remains a big challenge. Here, we report the unusual role of phosphorous in modulating the electrochemical N2 reduction activity of an Fe catalyst. An FeP2 nanoparticle-reduced graphene oxide hybrid (FeP2-rGO) attains a large NH3 yield of 35.26 µg h-1 mgcat.-1 (7.06 µg h-1 cm-2) and a high faradaic efficiency of 21.99% at -0.40 V vs. reversible hydrogen electrode in 0.5 M LiClO4, outperforming the FeP-rGO hybrid (17.13 µg h-1 mgcat.-1; 8.57%). Theoretical calculations reveal that FeP2 possesses decreased catalytic activity for the hydrogen evolution reaction, higher N2 adsorption energy, and a larger number of active sites than FeP.

19.
ACS Nano ; 13(11): 13492-13500, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31689076

RESUMO

Generating single photons at high temperature remains a major challenge, particularly for group III-As and III-P materials widely used in optical communication. Here, we report a high temperature single photon emitter based on a "surface-free" GaAs quantum dot (QD) in a GaAsP nanowire. By using self-catalyzed vapor-liquid-solid growth and simple surface engineering, we can significantly enhance the optical signal from the QDs with a highly polarized photoluminescence at 750 nm. The "surface-free" nanowire quantum dots show photon antibunching up to 160 K and well resolved exciton lines as high as 220 K.

20.
Angew Chem Int Ed Engl ; 58(51): 18449-18453, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31549471

RESUMO

Titanium-based catalysts are needed to achieve electrocatalytic N2 reduction to NH3 with a large NH3 yield and a high Faradaic efficiency (FE). One of the cheapest and most abundant metals on earth, iron, is an effective dopant for greatly improving the nitrogen reduction reaction (NRR) performance of TiO2 nanoparticles in ambient N2 -to-NH3 conversion. In 0.5 m LiClO4 , Fe-doped TiO2 catalyst attains a high FE of 25.6 % and a large NH3 yield of 25.47 µg h-1 mgcat -1 at -0.40 V versus a reversible hydrogen electrode. This performance compares favorably to those of all previously reported titanium- and iron-based NRR electrocatalysts in aqueous media. The catalytic mechanism is further probed with theoretical calculations.

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