RESUMO
Objective: To investigate the chemical constituents changes of Citrus reticulata pericarp before and after processing with vinegar,and to provide scientific basis for the study of active components in Citrus reticulata pericarp. Methods: Citrus reticulata pericarp was processed with vinegar according to the Zhongyao Paozhixue Cidian. The extracts of unprocessed and processed Citrus reticulata pericarp were detected by HPLC. Partial least squares-discriminate analysis( PLS-DA) was employed to reveal the discrimination between different samples. Results: 15 chemical constituents had significant changes in Citrus reticulata pericarp before and after processing with vinegar. Two components increased significantly after processing with vinegar were characterized as narirutin and hesperidin respectively. Conclusion: The chemical constituents in Citrus reticulata pericarp are found to be changed after processing with vinegar, two components with significant increasing indicate the basis of the variation of bioactivity in vinegar-processed Citrus reticulata pericarp, which provides evidence to interpret its clinical usage.
